ABSTRACT
BACKGROUND: Schinus terebinthifolius Raddi belongs to Anacardiacea family and is widely known as "aroeira." This species originates from South America, and its extracts are used in folk medicine due to its therapeutic properties, which include antimicrobial, anti-inflammatory, and antipyretic effects. The complexity and variability of the chemical constitution of the herbal raw material establishes the quality of the respective herbal medicine products. OBJECTIVE: Thus, the purpose of this study was to investigate the variability of the volatile compounds from leaves of S. terebinthifolius. MATERIALS AND METHODS: The samples were collected from different states of the Northeast region of Brazil and analyzed with a gas chromatograph coupled to a mass spectrometer (GC-MS). The collected data were analyzed using multivariate data analysis. RESULTS: The samples' chromatograms, obtained by GC-MS, showed similar chemical profiles in a number of peaks, but some differences were observed in the intensity of these analytical markers. The chromatographic fingerprints obtained by GC-MS were suitable for discrimination of the samples; these results along with a statistical treatment (principal component analysis [PCA]) were used as a tool for comparative analysis between the different samples of S. terebinthifolius. CONCLUSION: The experimental data show that the PCA used in this study clustered the samples into groups with similar chemical profiles, which builds an appropriate approach to evaluate the similarity in the phytochemical pattern found in the different leaf samples. SUMMARY: The leave extracts of Schinus terebinthifolius were obtained by turbo-extractionThe extracts were partitioned with hexane and analyzed by GC-MSThe chromatographic data were analyzed using the principal component analysis (PCA)The PCA plots showed the main compounds (phellandrene, limonene, and carene), which were used to group the samples from a different geographical location in accordance to their chemical similarity. Abbreviations used: AL: Alagoas, BA: Bahia, CE: Ceará, CPETEC: Center for Weather Forecasting and Climate Studies, GC-MS: Gas chromatograph coupled to a mass spectrometer, MA: Maranhão, MVA: Multivariate data analysis, PB: Paraíba, PC1: Direction that describes the maximum variance of the original data, PC2: Maximum direction variance of the data in the subspace orthogonal to PC1, PCA: Principal component analysis, PE: Pernambuco, PI: Piauí, RN: Rio Grande do Norte, SE: Sergipe.
ABSTRACT
O presente trabalho teve como objetivo avaliar a influência do adubo, da irrigação, da incidência solar, do horário de coleta e da idade da planta na quantidade de β-cariofileno no óleo essencial de Plectranthus amboinicus (Lour.) Spreng., Lamiaceae. As plantas foram cultivadas em canteiros experimentais entre os meses de dezembro de 2006 a setembro de 2007. O substrato utilizado foi adubo orgânico (esterco bovino), adubo mineral 1 [NPK (nitrogênio, fósforo e potássio)] e adubo mineral 2 (NPK com calcário) sob diferentes tratamentos. A técnica analítica quantitativa utilizada foi a cromatografia gasosa (GC/FID). De acordo com os resultados obtidos nos meses de menor precipitação de chuvas obteve-se maior rendimento de óleo essencial, e os meses de maior precipitação de chuvas mostraram uma tendência de baixos rendimentos.
This study aimed to evaluate the influence of fertilizer, irrigation, the incidence sun, the time of collection and the plant in the amount of β-caryophyllene in the essential oil of Plectranthus amboinicus (Lour.) Spreng., Lamiaceae. The plants were grown in experimental beds between the months of December 2006 to September 2007. The substrate was used organic fertilizer (esterco veal), fertilizer mineral 1 [NPK (nitrogen, phosphorus and potassium)] and fertilizer mineral 2 (NPK with limestone) under different treatments. The quantitative analytical technique was used to gas chromatography (GC/FID). According to the results obtained in months of lower precipitation of rainfall received are higher yield of essential oil, and the months of highest precipitation of rain showed a trend of low income.
