ABSTRACT
This report describes the clinical features and molecular diagnosis of a case of canine leproid granuloma (CLG) caused by mycobacterial strains of the Mycobacterium simiae complex in Brazil. A 12-year-old non-neutered male Labrador Retriever dog was presented with a 2-week history of progressive painless cutaneous lesions. Ulcerated nodules with hematic crusts were observed on the dorsal surface of the right and left pinna and on the metacarpal, metatarsal, and digits. Complete blood count, serum biochemistry, aspiration cytology of cutaneous lesions, biopsy for histopathological evaluation, culture for aerobic and anaerobic bacteria, polymerase chain reaction and DNA sequencing to identify mycobacterial species were performed. According to the clinical and histopathological findings, a diagnosis of CLG was established. Despite the negative result of the bacterial culture, mycobacterial identification was made by sequencing the hsp65 gene. Our findings highlight that mycobacterial species closely related to members of the M simiae clade can be causative agents of CLG.
Subject(s)
Dog Diseases , Mycobacterium Infections , Mycobacterium , Animals , Brazil , Dog Diseases/pathology , Dogs , Granuloma/microbiology , Granuloma/pathology , Granuloma/veterinary , Male , Mycobacterium/genetics , Mycobacterium Infections/diagnosis , Mycobacterium Infections/veterinaryABSTRACT
This report aims to describe one case of plasma cell pododermatitis associated with feline leukemia virus (FeLV) and concomitant feline immunodeficiency virus (FIV) infection in a cat. A 2-year-old, intact male, mixed-breed cat was presented with alopecia, skin peeling, and erythematous swelling in the left metacarpal paw pad. Swelling, softening, ulceration with secondary crusts, and erythematous to violaceous discoloration were observed in multiple metacarpal, metatarsal, and digital paw pads. Complete blood count and serum biochemistry were analyzed. FeLV antigenemia and FIV seropositivity were assessed by immunoassay (enzyme-linked immunosorbent assay). Nested-PCR was used to detect FIV and FeLV proviral DNA in blood cells. Histopathological examination and anti-FeLV and anti-FIV immunohistochemical were performed on paw pad biopsies. According to clinical and histopathological findings, a diagnosis of plasma cell pododermatitis was made. The cat was FIV and FeLV seropositive. The immunohistochemical of paw pad biopsies revealed FeLV positivity and FIV negativity. This study provides reference for further investigations about feline plasma cell pododermatitis and highlights retrovirus infection as a potential factor associated with this disease.
