ABSTRACT
El presente trabajo de investigación tiene como objetivo principal el aislar, expandir y caracterizar inmunofenotípicamente células madre mesenquimales de la pulpa dental humana, según los criterios mínimos propuestos por The International Society for Cellular Therapy (ISCT), como así también establecer la puesta a punto de las técnicas y protocolos de procedimientos para tal fin. Los cultivos fueron permanentemente monitoreados mediante microscopio invertido con contraste de fase y la inmunotipificación fue realizada por citometría de flujo (AU)
Subject(s)
Humans , Male , Female , Tissue Engineering , Dental Pulp , Adult Stem Cells , Mesenchymal Stem Cells , Phenotype , Argentina , Schools, Dental , Cell Culture Techniques , Regenerative MedicineABSTRACT
The preparation from w/o microemulsion of polybutylcianacrylate nanocapsules incorporating doxorubicine was optimized applying a multivariate experimental design. Different variables were investigated; the responses were polymer yield, concentration of the drug in the particles and percentage of the drug incorporated. A preliminary study was carried out with the strategy of fractional factorial design; the following step was a central composite design in order to study the most important causal variables. The information collected were treated by the response surface method (REGFAC package). The optimization allowed to maximize the responses; an incorporation of about 10% of doxorubicine and a yield of about 95% were obtained, starting from values notably lower of both incorporation and yield.
Subject(s)
Doxorubicin/chemistry , Doxorubicin/administration & dosage , Enbucrilate/chemistry , MicrospheresABSTRACT
Synopsis The optimization of three emulsions with predetermined components was performed by the aid of experimental design. Three different surfactants were compared: the salts of hexadecylhydrogenphosphate with diethanolamine, lysine and arginine respectively. Optimized microemulsions showed quite high stabilities towards centrifugation (30-60 min at 7000 rpm). The results showed that the counter-ion can affect the composition and stability of an emulsion.
