ABSTRACT
Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).
Subject(s)
Dengue Virus/classification , Dengue Virus/genetics , Dengue/diagnosis , Dengue/virology , Molecular Diagnostic Techniques/methods , Multiplex Polymerase Chain Reaction/methods , Real-Time Polymerase Chain Reaction/methods , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Sensitivity and Specificity , Serogroup , Trinidad and Tobago , Young AdultABSTRACT
The prevalence and socio-biological relations of bacteriuria was found to be 16.7 percent and it was more common in the 30-39 age group, among parous women, among negroes, and in patients with a low family income and overcrowded living conditions. Symptoms were present in 19 percent of bacteriuric patients and almost one-third gave a past history of urinary tract infection. Only 10 percent had been previously exposed to sexually transmitted diseases such as syphilis, gonorrhoea and herpetic genital infections. Because of the serious consequences to mother and foetus, we advocate quantitative urine cultures for all antenatal patients, especially those from disadvantaged socio-economic conditions (AU)
Subject(s)
Humans , Female , Pregnancy , Adolescent , Adult , Bacteriuria/epidemiology , Pregnancy Complications, Infectious/epidemiology , Trinidad and Tobago/epidemiology , Socioeconomic Factors , Risk Factors , Urine , Bacteriuria/complications , Prenatal Care , Prospective StudiesABSTRACT
The prevalence and socio-biological relations of bacteriuria was found to be 16.7 per cent and it was more common in the 30-39 age group, among parous women, among negroes, and in patients with a low family income and overcrowded living conditions. Symptoms were present in 19 per cent of bacteriuric patients and almost one-third gave a past history of urinary tract infection. Only 10 per cent had been previously exposed to sexually transmitted diseases such as syphilis, gonorrhoea and herpetic genital infections. Because of the serious consequences to mother and foetus, we advocate quantitative urine cultures for all antenatal patients, especially those from disadvantaged socio-economic conditions
Subject(s)
Humans , Female , Pregnancy , Adolescent , Adult , Pregnancy Complications, Infectious/epidemiology , Bacteriuria/epidemiology , Prenatal Care , Socioeconomic Factors , Bacteriuria/complications , Trinidad and Tobago/epidemiology , Urine , Prospective Studies , Risk FactorsABSTRACT
In Trinidad and Tobago, gastroenteritis is regarded as a major health problem and an attempt was made to determine the role of pipeborne water in the spread of the disease in some areas of the country. Eighteen children in an area which every year has a high incidence of gastroenteritis and thirteen children in an area of low incidence of the disease were used in the study. Stool samples from the children and water samples from taps in their homes were collected on a weekly basis. All the children were four years and under and lived in middle/low socioeconomic areas. Salmonellae and Shigellae were isolated on three occasions from water and seventeen occasions from stool samples but the tap water was not found to be responsible for symptomatic and asymptomatic infections in any of the children. Stool samples from children suffering from gastroenteritis in the area of highest incidence of the disease in the countryand water samples from all the standpipes serving the area were collected on a fortnightly basis. Salmonellae and Shigellae were isolated from tap water but not from stool samples. It was therefore concluded that tap water was not responsible for the disease in this area. (AU)
