ABSTRACT
The use of personal computers in accountancy and business generally has been stimulated by the availability of flexible software packages. We describe the implementation of a commercial software package designed for interfacing with laboratory instruments and highlight the ease with which it can be implemented, without the need for specialist computer programming staff.
Subject(s)
Clinical Laboratory Information Systems/instrumentation , Information Systems/instrumentation , Microcomputers , Software , User-Computer Interface , Equipment Design , Pathology Department, HospitalSubject(s)
Antigens, Viral/isolation & purification , Immunoassay , Simplexvirus/immunology , HumansSubject(s)
Infectious Mononucleosis/complications , Porphyrias/enzymology , Porphyrins/urine , Skin Diseases/enzymology , Uroporphyrins/urine , Viremia/complications , Child , Erythrocytes/enzymology , Humans , Male , Porphyrias/genetics , Skin Diseases/genetics , Uroporphyrinogen Decarboxylase/deficiencyABSTRACT
Ethanol in blood was assayed by 2 currently available procedures: a commercial kit enzymatic assay and a gas chromatographic procedure. Both methods were performed with commercially available materials and equipment. Results by the 2 procedures agreed well. For the 192 specimens studied, the correlation coefficient was 0.983, and the least-squares values of slope and intercept were 0.999 and 0.001 g/100 ml respectively. Use of isopropanol or ethanol swabs did not affect results of the enzymatic procedure.
Subject(s)
Alcohol Oxidoreductases/blood , Alcoholic Intoxication/diagnosis , Chromatography, Gas , Ethanol/blood , Reagent Kits, Diagnostic , Alcohol Dehydrogenase , Alcoholic Intoxication/enzymology , HumansABSTRACT
We describe an improved gas chromatographic method for the determination of 3-methoxy-4-hydroxymandelic acid (vanilmandelic acid), 3-methoxy-4-hydroxyphenyacetic-acid (homovanillic acid), and 3-methoxy-4-hydroxyphenylethylene glycol. By incorporating slight modifications to established procedures, this method allows the urinary metabolites to be measured more rapidly and with better resolution. The procedure uses an internal standard and involves ethyl acetate extraction, silylation, and chromatography using a combination of two different stationary phases. The technique is simple and reliable and may be easily incorporated as a routine laboratory procedure.
Subject(s)
Catecholamines/urine , Chromatography, Gas/methods , Hippurates/urine , Homovanillic Acid/urine , Humans , Methoxyhydroxyphenylglycol/urine , Vanilmandelic Acid/urineABSTRACT
A method is described for the simultaneous determination of urinary pregnanediol and 17-ketosteroids by gas chromatography and of total urinary oestrogens by fluorimetry, all from the same urinary extract. The technique is simple, rapid and reliable, incorporating slight modifications to established procedures.
Subject(s)
17-Ketosteroids/urine , Estrogens/urine , Pregnanediol/urine , Chromatography, Gas , Fluorometry , Humans , MethodsABSTRACT
Biochemical investigations done on the blood and urine of currently healthy, uncomplicated patients previously treated by either proctocolectomy and ileostomy or colectomy and ileorectal anastomosis for ulcerative colitis were compared with estimations on matched controls. The most striking finding was the reduced urine volume, and to a lesser extent its sodium content, in patients with ileostomies. The urinary pH indicated persistent acidity in postoperative patients but this was also a remarkably constant feature in control subjects. These findings are in agreement with other studies related to the occurrence of urinary calculi after colectomy and ileostomy, and are consistent with the contention that there is a lower incidence of calculi after ileorectal anastomosis.
