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1.
J Fish Biol ; 91(3): 750-763, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28776696

ABSTRACT

A new species of Hyphessobrycon from the upper Rio Tapajós basin, in the Tapajós-Juruena ecoregion, is described. Hyphessobrycon pinnistriatus n. sp. is distinguished from its congeners by having a black, oblique stripe extending from the origin of the second branched ray to the distal end of the third branched anal-fin ray, lacking a conspicuous black midlateral stripe on the body, inner premaxillary teeth with up to seven cusps, and fins normally hyaline or with scattered chromatophores. The description of a new species that is restricted to the Tapajós-Juruena ecoregion is consistent with this region being an area of high endemism of freshwater fishes.


Subject(s)
Characiformes/classification , Animal Fins , Animals , Brazil , Characiformes/anatomy & histology , Color , Female , Fresh Water , Male , Rivers , Sex Characteristics , Tooth
2.
Transplant Proc ; 48(7): 2310-2314, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27742286

ABSTRACT

BACKGROUND: Urine monitoring programs represent an important strategy for early diagnosis of reactivation of BK polyomavirus (BKV) in kidney transplant recipients. This study analyzes a BKV urine screening model in kidney transplant patients. METHODS: Urinary screening for BKV reactivation was performed by urinary decoy cell and polymerase chain reaction (PCR) tests in samples from 32 consecutive kidney transplant patients, collected in a 6-month follow-up period. PCR in plasma samples and BKV immunohistochemical studies to assess BKV renal disease, if a kidney biopsy was indicated, were performed. RESULTS: The urinary screening for BKV among 32 renal receptors was positive in 18 patients (56%) by the concomitant use of the decoy cells and/or qualitative PCR at some time during the study period. Transfusion before transplantation was significantly associated with urinary decoy cell positive screening (odds ratio = 11; 95% confidence interval = 1.47 to 82.4; P < .05); and so was male sex (odds ratio = 2.02; 95% confidence interval = 1.07 to 3.83; P < .05). The clinical management of screening positive cases consisted of decreasing or changing the immunosuppression regimen. Sixteen renal biopsies were performed. Immunohistochemistry for SV40 T antigen was negative in all biopsies. After 1 year of follow-up, no patient developed BKV-associated nephropathy, and there was no difference in renal function between patients positive and negative for BKV urinary screening. CONCLUSIONS: Early urinary monitoring is effective in detection of BKV replication and represents a good strategy to minimize the deleterious effects caused by the presence of the virus on preservation of graft function.


Subject(s)
DNA, Viral/urine , Kidney Diseases/urine , Kidney Transplantation , Polyomavirus Infections/urine , Tumor Virus Infections/urine , Adult , BK Virus/genetics , Biopsy , Female , Graft Rejection/prevention & control , Humans , Immunohistochemistry , Immunosuppressive Agents/adverse effects , Kidney Diseases/diagnosis , Kidney Diseases/etiology , Kidney Diseases/pathology , Male , Mass Screening , Middle Aged , Odds Ratio , Pilot Projects , Polymerase Chain Reaction , Polyomavirus Infections/diagnosis , Polyomavirus Infections/etiology , Sex Factors , Transplant Recipients , Tumor Virus Infections/diagnosis , Tumor Virus Infections/etiology , Urinalysis
3.
Clin Microbiol Infect ; 17(4): 603-9, 2011 Apr.
Article in English | MEDLINE | ID: mdl-20456457

ABSTRACT

Acute ocular infection due to free-living amoebae of the genus Acanthamoeba is characterized by severe pain, loss of corneal transparency and, eventually, blindness. Proteolytic enzymes secreted by trophozoites of virulent Acanthamoeba strains have an essential role in the mechanisms of pathogenesis, including adhesion, invasion and destruction of the corneal stroma. In this study, we analysed the relationship between the extracellular proteases secreted by clinical isolates of Acanthamoeba and the clinical manifestations and severity of disease that they caused. Clinical isolates were obtained from patients who showed typical symptoms of Acanthamoeba keratitis. Trophozoites were cultivated axenically, and extracellular proteins were collected from cell culture supernatants. Secreted enzymes were partially characterized by gelatin and collagen zymography. Acanthamoeba trophozoites secreted proteases with different molecular masses, proteolysis rates and substrate specificities, mostly serine-like proteases. Different enzymatic patterns of collagenases were observed, varying between single and multiple collagenolytic activities. Low molecular weight serine proteases were secreted by trophozoites associated with worse clinical manifestations. Consequently, proteolytic enzymes of some Acanthamoeba trophozoites could be related to the degree of their virulence and clinical manifestations of disease in the human cornea.


Subject(s)
Acanthamoeba Keratitis/pathology , Acanthamoeba Keratitis/parasitology , Acanthamoeba/enzymology , Protozoan Proteins/metabolism , Serine Proteases/metabolism , Acanthamoeba/isolation & purification , Adult , Electrophoresis , Humans , Middle Aged , Molecular Weight , Protozoan Proteins/chemistry , Protozoan Proteins/isolation & purification , Serine Proteases/chemistry , Serine Proteases/isolation & purification , Severity of Illness Index , Statistics as Topic , Substrate Specificity
4.
Article in English | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1062088

ABSTRACT

Acute ocular infection due to free-living amoebae of the genus Acanthamoeba is characterized by severe pain, loss of corneal transparency and, eventually, blindness. Proteolytic enzymes secreted by trophozoites of virulent Acanthamoeba strains have an essential role in the mechanisms of pathogenesis, including adhesion, invasion and destruction of the corneal stroma. In this study, we analysed the relationship between the extracellular proteases secreted by clinical isolates of Acanthamoeba and the clinical manifestations and severity of disease that they caused. Clinical isolates were obtained from patients who showed typical symptoms of Acanthamoeba keratitis. Trophozoites were cultivated axenically, and extracellular proteins were collected from cell culture supernatants. Secreted enzymes were partially characterized by gelatin and collagen zymography. Acanthamoeba trophozoites secreted proteases with different molecular masses, proteolysis rates and substrate specificities, mostly serine-like proteases. Different enzymatic patterns of collagenases were observed, varying between single and multiple collagenolytic activities. Low molecular weight serine proteases were secreted by trophozoites associated with worse clinical manifestations. Consequently, proteolytic enzymes of some Acanthamoeba trophozoites could be related to the degree of their virulence and clinical manifestations of disease in the human cornea.


Subject(s)
Male , Female , Humans , Acanthamoeba , Acanthamoeba Keratitis
5.
J. venom. anim. toxins incl. trop. dis ; 16(2): 324-341, 2010. ilus, graf
Article in English | LILACS, Sec. Est. Saúde SP | ID: lil-548853

ABSTRACT

The aim of the present work was to study the effect of the crude extract of Curcuma zedoaria on peripheral blood cells and tumor progression in C57Bl/6J mice injected with B16F10 murine melanoma cells. The intraperitoneal therapy showed a significant increase in total white and red blood cell counts, a decrease in peritoneal cell number and tumor volume reduction, whereas the oral administration revealed a noteworthy augmentation only in total leukocyte count. These results contribute to evaluate the importance of alternative treatments that employ phytotherapic compounds against tumor progression and its possible immunomodulation.


Subject(s)
Animals , Curcuma , Immunologic Factors , Melanoma/chemically induced , Mice , Phytotherapy
6.
Cornea ; 28(5): 516-9, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19421047

ABSTRACT

PURPOSE: We described the rate of Acanthamoeba keratitis (AK) in a referral eye center in São Paulo, Brazil, through a retrospective review of clinical and laboratorial records of patients over 2 decades. METHODS: From 1987 to 2006, a total of 581 requests for amoebic laboratory workup in cases of infectious keratitis were investigated. Statistical analyses were applied to analyze a tendency of AK cases. RESULTS: Acanthamoeba species were cultured from corneal scrapings of 185 patients, 5 of them with bilateral infection. Eighty-three percent of those patients were related with contact lens wear. CONCLUSIONS: The results suggested that patients with AK have persisted and increased over time at our ophthalmology center. Contact lenses showed to be a potential risk factor. Amoebic corneal infection can be considered as a new but well-established disease in Brazilian ophthalmology and visual sciences.


Subject(s)
Acanthamoeba Keratitis/epidemiology , Acanthamoeba Keratitis/etiology , Brazil/epidemiology , Contact Lenses/adverse effects , Eye Foreign Bodies/complications , Female , Humans , Incidence , Male , Prevalence , Retrospective Studies , Sex Distribution
7.
Cochrane Database Syst Rev ; (2): CD005515, 2007 Apr 18.
Article in English | MEDLINE | ID: mdl-17443597

ABSTRACT

BACKGROUND: Anterior open bite occurs when there is a lack of vertical overlap of the upper and lower incisors. The aetiology is multifactorial including: oral habits, unfavourable growth patterns, enlarged lymphatic tissue with mouth breathing. Several treatments have been proposed to correct this malocclusion, but interventions are not supported by strong scientific evidence. OBJECTIVES: The aim of this systematic review was to evaluate orthodontic and orthopaedic treatments to correct anterior open bite in children. SEARCH STRATEGY: Search strategies were developed for MEDLINE and revised appropriately for the following databases: Cochrane Oral Health Group Trials Register; CENTRAL (The Cochrane Library 2005, Issue 4); PubMed (1966 to December 2005); EMBASE (1980 to February 2006); Lilacs (1982 to December 2005); Brazilian Bibliography of Odontology (BBO) (1986 to December 2005); and SciELO (1997 to December 2005). Chinese journals were handsearched and the bibliographies of papers were retrieved. SELECTION CRITERIA: All randomised or quasi-randomised controlled trials of orthodontic or orthopaedic treatments or both to correct anterior open bite in children. DATA COLLECTION AND ANALYSIS: Two review authors independently assessed the eligibility of all reports identified. Risk ratios (RRs) and corresponding 95% confidence intervals (CIs) were calculated for dichotomous data. The continuous data were expressed as described by the author. MAIN RESULTS: Twenty-eight trials were potentially eligible, but only three randomised controlled trials were included comparing: effects of Frankel's function regulator-4 (FR-4) with lip-seal training versus no treatment; repelling-magnet splints versus bite-blocks; and palatal crib associated with high-pull chincup versus no treatment. The study comparing repelling-magnet splints versus bite-blocks could not be analysed because the authors interrupted the treatment earlier than planned due to side effects in four of ten patients.FR-4 associated with lip-seal training (RR = 0.02 (95% CI 0.00 to 0.38)) and removable palatal crib associated with high-pull chincup (RR = 0.23 (95% CI 0.11 to 0.48)) were able to correct anterior open bite.No study described: randomisation process, sample size calculation, there was not blinding in the cephalometric analysis and the two studies evaluated two interventions at the same time. These results should be therefore viewed with caution. AUTHORS' CONCLUSIONS: :There is weak evidence that the interventions FR-4 with lip-seal training and palatal crib associated with high-pull chincup are able to correct anterior open bite. Given that the trials included have potential bias, these results must be viewed with caution. Recommendations for clinical practice cannot be made based only on the results of these trials. More randomised controlled trials are needed to elucidate the interventions for treating anterior open bite.


Subject(s)
Open Bite/therapy , Orthodontics, Corrective/methods , Orthopedic Procedures/methods , Adolescent , Child , Humans , Malocclusion/therapy , Orthodontic Appliances, Functional , Orthodontic Appliances, Removable , Randomized Controlled Trials as Topic
8.
Cochrane Database Syst Rev ; (2): CD005520, 2007 Apr 18.
Article in English | MEDLINE | ID: mdl-17443598

ABSTRACT

BACKGROUND: Apnoea is a breathing disorder marked by the absence of airflow at the nose or mouth. In children, risk factors include adenotonsillar hypertrophy, obesity, neuromuscular disorders and craniofacial anomalies. The most common treatment for obstructive sleep apnoea syndrome (OSAS) in childhood is adenotonsillectomy. This approach is limited by its surgical risks, mostly in children with comorbities and, in some patients, by recurrence that can be associated with craniofacial problems. Oral appliances and functional orthopaedic appliances have been used for patients who have OSAS and craniofacial anomalies because they change the mandible posture forwards and potentially enlarge the upper airway and increase the upper airspace, improving the respiratory function. OBJECTIVES: To assess the effectiveness of oral appliances or functional orthopaedic appliances for OSAS in children. SEARCH STRATEGY: A sensitive search was developed for the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2005, Issue 3); PubMed (January 1966 to September 2005); EMBASE (1980 to September 2005); Lilacs (1982 to September 2005); BBO-Bibliografia Brasileira de Odontologia (1986 to September 2005); and SciELO (1997 to September 2005). There was no restriction of language or source of information. SELECTION CRITERIA: All randomised or quasi-randomised controlled trials comparing all types of oral and functional orthopaedic appliances with placebo or no treatment, in children 15 years old or younger. PRIMARY OUTCOME: reduction of apnoea to less than one episode per hour. SECONDARY OUTCOMES: dental and skeletal relationship, sleep parameters improvement, cognitive and phonoaudiologic function, behavioural problems, drop outs and withdrawals, quality of life, side effects (tolerability), economic evaluation. DATA COLLECTION AND ANALYSIS: Data were independently extracted by two review authors. Authors were contacted for additional information. Risk ratios with 95% confidence intervals were calculated for all important dichotomous outcomes. MAIN RESULTS: The initial search identified 384 trials. One of them, reporting results from a total of 23 patients, was suitable for inclusion in the review. Data provided in the published report did not answer all the questions from this review, but some of them were, and the presented results favour treatment. AUTHORS' CONCLUSIONS: At present there is no sufficient evidence to state that oral appliances or functional orthopaedic appliances are effective in the treatment of OSAS in children. Oral appliances or functional orthopaedic appliances may be helpful in the treatment of children with craniofacial anomalies which are risk factors for apnoea.


Subject(s)
Orthodontic Appliances, Functional , Orthodontic Appliances, Removable , Sleep Apnea, Obstructive/therapy , Adolescent , Child , Humans
9.
Acta Med Port ; 11(2): 153-61, 1998 Feb.
Article in Portuguese | MEDLINE | ID: mdl-9567412

ABSTRACT

The authors describe ten years of experience in breast reconstruction in the Department of Plastic and Reconstructive Surgery in the District Hospital of Santarém--Portugal. Ninety-nine patients were treated with 106 reconstructions, most of these were immediate and some were delayed. The authors were interested in providing an adequate volume, but also into obtaining an acceptable aesthetic result. This contributes considerably to the psychological recovery of the patients.


Subject(s)
Breast Neoplasms/surgery , Mammaplasty/methods , Surgical Flaps , Breast Implantation , Female , Humans , Mastectomy
10.
Acta Med Port ; 11(11): 953-9, 1998 Nov.
Article in Portuguese | MEDLINE | ID: mdl-10021794

ABSTRACT

Cutaneous squamous cell carcinoma is the most common metastatic skin cancer. The importance of early recognition and thorough treatment of premalignant lesions as well as the recognition of risk factors of the neoplasms that are most likely to metastasize must also be emphasized. A retrospective study of 126 patients, treated for primary squamous cell carcinoma of the face, was undertaken over a 7-year period. An attempt is made to define the major therapeutic modalities chosen, taking into consideration the specific anatomic location.


Subject(s)
Carcinoma, Squamous Cell/therapy , Facial Neoplasms/therapy , Skin Neoplasms/therapy , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Retrospective Studies
12.
Angiology ; 35(2): 63-70, 1984 Feb.
Article in English | MEDLINE | ID: mdl-6696288

ABSTRACT

Endomyocardial fibrosis (EMF) is a severe restrictive cardiomyopathy characterized by the formation of fibrous tissue on the endocardium and to a lesser extent in the myocardium of one or both ventricles. The disease results in endocardial rigidity, atrioventricular valve dysfunction and progressive obliteration of the ventricular cavity. The clinical and pathological findings of EMF have been well established and definitive diagnosis can be made by selective cineangiocardiography. However, there are few reports concerning the phonomechanocardiographic (PMCG) patterns in this condition. In this paper we describe the PMCG findings in 12 patients with EMF which is now recognized with increasing frequency in the Northeast of Brazil.


Subject(s)
Endomyocardial Fibrosis/diagnosis , Phonocardiography , Adult , Atrioventricular Node/physiopathology , Child , Endomyocardial Fibrosis/physiopathology , Female , Heart Sounds , Heart Ventricles/physiopathology , Humans , Kinetocardiography , Male , Pulse
13.
Arq. bras. cardiol ; 38(3): 181-5, 1982.
Article in Portuguese | LILACS | ID: lil-8779

ABSTRACT

Estudou-se a amplitude da onda "a" em relacao a da onda "v" (A/V), do pulso venoso jugular (PVJ) em um grupo de 60 pacientes portadores de estenose pulmonar (GEP) e em um de 30 pessoas normais (GN). O GEP foi dividido em dois subgrupos: o de 36 pacientes portadores de estenose pulmonar pura (GEPP) e o de 24 pacientes portadores de estenose pulmonar associada a defeito septal (GEPS). No GN, o valor A/V foi de 1,27 +/- 0,17 (media +/- desvio-padrao), enquanto no gep foi de 1,81 +/- 0,51, com diferenca altamente significativa comparada com o GN (p < 0,0001). No GEPP, o valor foi de 1,88 +/- 0,51, enquanto que no GEPS foi de 1,70 +/- 0,50, mostrando pequena diferenca entre as medias, porem nnao significativa (p < 0,05). Contudo, ambos subgrupos apresentaram uma diferenca altamente significativa comparados ao GN (P < 0,0001). Relacionando-se o GEP com o GN, observou-se que em 63% dos casos A/V estava anormalmente aumentada, sendo que a pressao sistolica de ventriculo direto (PSVO) era maior que 50 mmHg em todos eles. Alem disso, quando a relacao A/V foi maior ou igual a 2,00, a PSVD era maior ou igual a 80,00 mmHg em 95% dos casos.


Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Pulmonary Valve Stenosis , Pulse , Jugular Veins
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