Influence of the freezing level on atmospheric rivers in High Mountain Asia: WRF case studies of orographic precipitation extremes.

Atmospheric rivers (ARs) reach High Mountain Asia (HMA) about 10 days per month during the winter and spring, resulting in about 20 mm day-1 of precipitation. However, a few events may exceed 100 mm day-1, providing most of the total winter precipitation and increasing the risk of precipitation-triggered landslides and flooding, particularly when the height of the height of the 0 ∘C isotherm, or freezing level is above-average. This study shows that from 1979 to 2015, integrated water vapor transport (IVT) during ARs that reach Western HMA has increased 16% while the freezing level has increased up to 35 m. HMA ARs that have an above-average freezing level result in 10-40% less frozen precipitation compared to ARs with a below-average freezing level. To evaluate the importance of these trends in the characteristics of ARs, we investigate mesoscale processes leading to orographic precipitation using Advanced Weather Research and Forecasting (ARW-WRF) simulations at 6.7 km spatial resolution. We contrast two above- and below- average freezing level AR events with otherwise broadly similar characteristics and show that with a 50-600 m increase in freezing level, the above-average AR resulted in 10-70% less frozen precipitation than the below-average event. This study contributes to a better understanding of climate change-related impacts within HMA's hydrological cycle and the associated hazards to vulnerable communities living in the region.

Deciphering the underlying mechanisms of the oxidative perturbations and impaired meat quality in Wooden breast myopathy by label-free quantitative MS-based proteomics.

The study aimed to investigate biochemical mechanisms occurred in Wooden breast (WB) chicken meat, with attention to the impact on meat quality. Commercial chicken breasts were classified as Normal (N, n = 12), WB-M (moderate degree; focal hardness on cranial region, n = 12) and WB-S (severe degree; extreme and diffused hardness over the entire surface, n = 12). Samples were analyzed for physico-chemical properties, oxidative damage to lipids and proteins, and discriminating sarcoplasmic proteins by using a Q-Exactive mass spectrometer. WB meat presented impaired composition and functionality and higher levels of lipid and protein oxidation markers than N meat. The proteomic profile of WB-S presents a dynamic regulation of the relevant proteins involved in redox homeostasis, carbohydrate, protein and lipid metabolisms. Proteomics results demonstrate that the physiological and metabolic processes of muscles affected by WB myopathy are involved in combating the inflammatory process and in repairing the damaged tissue by oxidative stress.

Winter and spring atmospheric rivers in High Mountain Asia: climatology, dynamics, and variability.

Atmospheric rivers (ARs) that reach the complex terrain of High Mountain Asia (HMA) cause significant hydrological impacts for millions of people. While ARs are often associated with precipitation extremes and can cause floods and debris flows affecting populated communities, little is known about ARs that reach as far inland as HMA. This paper characterizes AR types and investigates dynamical mechanisms associated with the development of ARs that typically affect HMA. Combined empirical orthogonal function (cEOF) analysis using integrated water vapor transport (IVT) is applied to days where an AR reaches HMA. K-means cluster analysis applied to the first two principal components uncovered three subtypes of AR events with distinct synoptic characteristics during winter and spring months. The first subtype increases precipitation and IVT in Western HMA and is associated with a zonally oriented wave train propagating within the westerly jet waveguide. The second subtype is associated with enhanced southwesterly IVT, anomalous upper-level cyclonic circulation centered on 45 ∘ E, and precipitation in Northwestern HMA. The third subtype shows anomalous precipitation in Eastern HMA and southwesterly IVT across the Bay of Bengal. Interannual variations in the frequency of HMA ARs and relationships with various teleconnection patterns show that western HMA AR subtypes are sensitive to well-known remote large-scale climate factors, such as the El Niño Southern Oscillation, Arctic Oscillation, and the Siberian High. These results provide synoptic characterization of the three types of ARs that reach HMA and reveal the previously unexplored significance of their contribution to winter and spring precipitation. Supplementary Information: The online version contains supplementary material available at 10.1007/s00382-021-06008-z.

Improving the poor texture and technological properties of chicken wooden breast by enzymatic hydrolysis and low-frequency ultrasound.

Wooden breast (WB) is a recurrent myopathy in fast-growing birds, which alters the appearance, functionality, and the texture of the breast muscle. The objectives of this study were (i) to evaluate the effect of a combined use of papain enzyme and ultrasound on the texture of WB chicken using response surface methodology and (ii) to assess the effect of marinating on the quality of WB chicken meat. Full factorial experimental design method was used to obtain the ideal conditions to soften the WB meat. The independent variables were the concentration of papain (0.1%-0.3%) and the time in ultrasonic bath (10-30 min); shear force (SF) was the dependent variable. The optimum results were obtained at a concentration of 0.2% papain and 20 min on ultrasound. Papain enzyme had a great influence on the texture of WB meat, reducing its hardness. However, the effect of the ultrasound time on the SF response was not observed. The marinated WB meat showed similar SF values and texture profile than those from normal (N) meat, with reduction in the parameters of protein and lipid oxidation. The use of papain without ultrasound bath proved to be an efficient means for improving the tenderness of WB breasts. PRACTICAL APPLICATION: This study shows the efficiency of the application of two technological procedures (enzymatic treatment and ultrasound) to improve the texture profile and technological properties of chicken breasts affected by the wooden breast myopathy. The economic loss caused by the world-wide occurrence of wooden breast is enormous, and the application of papain has been found to counteract the impaired properties of this abnormal chicken breasts. Since papain is already widely used in the food industry to tenderize meat, its application in improving the quality of WB meat is straightforward.

Reimagine fire science for the anthropocene.

Fire is an integral component of ecosystems globally and a tool that humans have harnessed for millennia. Altered fire regimes are a fundamental cause and consequence of global change, impacting people and the biophysical systems on which they depend. As part of the newly emerging Anthropocene, marked by human-caused climate change and radical changes to ecosystems, fire danger is increasing, and fires are having increasingly devastating impacts on human health, infrastructure, and ecosystem services. Increasing fire danger is a vexing problem that requires deep transdisciplinary, trans-sector, and inclusive partnerships to address. Here, we outline barriers and opportunities in the next generation of fire science and provide guidance for investment in future research. We synthesize insights needed to better address the long-standing challenges of innovation across disciplines to (i) promote coordinated research efforts; (ii) embrace different ways of knowing and knowledge generation; (iii) promote exploration of fundamental science; (iv) capitalize on the "firehose" of data for societal benefit; and (v) integrate human and natural systems into models across multiple scales. Fire science is thus at a critical transitional moment. We need to shift from observation and modeled representations of varying components of climate, people, vegetation, and fire to more integrative and predictive approaches that support pathways toward mitigating and adapting to our increasingly flammable world, including the utilization of fire for human safety and benefit. Only through overcoming institutional silos and accessing knowledge across diverse communities can we effectively undertake research that improves outcomes in our more fiery future.

Pinpointing oxidative stress behind the white striping myopathy: depletion of antioxidant defenses, accretion of oxidized proteins and impaired proteostasis.

BACKGROUND: This study aimed to investigate the molecular mechanisms involved in the onset of the white striping (WS) myopathy with particular attention to the role of oxidative stress and protein oxidation in the loss of meat quality. RESULTS: It was found that WS-M (moderate degree; white stripes <1 mm thickness) and WS-S (severe degree; white stripes >1 mm thickness) breast presented higher pH, hardness, redness, lipid, and collagen content, and lower lightness than normal breast. Compared with the latter, WS-S had a more severe loss of protein thiols (70.7% less thiols than in N), reduced activity of antioxidant enzymes such as catalase (23 versus 40 U g-1 ), glutathione peroxidase (0.21 versus 0.54 U g-1 ), and superoxide dismutase (56 versus 73 U g-1 ), and consequently, had greater accretion of thiobarbituric acid reactive substances (0.64 versus 0.22 mg MDAkg-1 muscle), allysine (3.1 versus 1.9 nmol mg-1 protein) and Schiff base structures (645 versus 258 fluorescent units). The analysis of sarcoplasmic proteins revealed that muscles severely affected by the myopathy suffered a chronic impairment of physiological (upregulation of sarcoplasmic reticulum Ca2+ ATPase, sarcalumenin and calsequestrin-2) and metabolic processes (downregulation of pyruvate kinase, creatine kinase, and l-lactate dehydrogenase). CONCLUSION: The overexpression of ribonuclease / angiogenin inhibitor 1 and Kelch-like proteins in WS chicken breasts indicates altered protein turnover plausibly mediated by oxidative stress and accumulation of oxidized proteins. © 2020 Society of Chemical Industry.

Causality of Biodiversity Loss: Climate, Vegetation, and Urbanization in China and America.

Essential for directing conservation resources is to identify threatened vertebrate regions and diagnose the underlying causalities. Through relating vertebrates and threatened vertebrates to the rainfall-runoff chain, to the food chain, and to the human impact of urbanization, the following relationships are noticed: (i) The Earth's vertebrates generally show increasing abundance and decreasing threatened species indicator (threatened species number/species abundance) for a higher Normalized Difference Vegetation Index (NDVI) or larger city-size. (ii) Regional vertebrates reveal a notable 'U-shape profile' ('step-like jump') of threatened species indicator occurs in the moderate (high) NDVI regions in China (America). (iii) Positive/green city states emerge in China and are characterized by the lowest threatened species indicators in areas of low to moderate greenness, where the greenness trend of change during the last 30 years is about three times higher in the urbanized areas than over land. (iv) Negative/brown city states emerge in America revealing high threatened species indicators for greenness exceeding NDVI > 0.2, where similar greenness trends are of both urbanized and land areas. The occurrence of green and brown city states suggests a biodiversity change pattern characterized by the threatened species indicator declining from city regimes with high to those with low indicator values for increasing ratio of the city-over-land NDVI trends.

Further insight into the role of Ca2+ in broiler pale, soft and exudative-like (PSE) meat through the analysis of moisture by TGA and strong cation elements by ICP-OES.

This study evaluated moisture content of broiler breast PSE meat by thermal gravimetric analysis and strong cation elements by inductively coupled plasma optical emission spectrometry. The weight changes were monitored within the temperature interval 9-750 °C and the most severe changes were observed at 9-160 °C (1A). This remarkable transition was attributed to water loss of about 64.74% in normal samples while PSE samples lost about 61.16%. There was, therefore, approximately 4.0% lower moisture in PSE meat samples in relation to normal meat. The analysis of cation elements showed significant differences (p < 0.05), with normal samples having 13.0% higher ∑[Ca2+] + [Mg2+] + [Na+]+[K+] than PSE meat samples while [Ca2+] in PSE meat was 10.81% higher than in the normal counterparts. We provided further evidence of the role of Ca2+ excess within the muscle sarcomere during the onset of PSE meat.