ABSTRACT
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.
Subject(s)
Checklist , Publishing , Reproducibility of Results , Image Processing, Computer-Assisted , MicroscopyABSTRACT
We report the operation and characterization of an upconversion random laser emitting at 560 nm, when directly pumped by three photon excitation at the near IR wavelength of 1350 nm in a colloidal dye solution in the weakly scattering regime. Using a special dye with a high three-photon cross-section and TiO(2) nanoparticles (250 nm diameter), optimized upconverted emission was obtained for particle densities of ~2 x 10(9)/cm3. A strong dependence on the nanoparticle concentration and the pumping area was verified. The presence of spikes with linewidths ~0.4 nm in the emitted spectrum is the signature of coherent emission from this three-photon pumped random laser.
