Screening of Chemical Composition, Antimicrobial and Antioxidant Activities in Pomegranate, Quince, and Persimmon Leaf, Peel, and Seed: Valorization of Autumn Fruits By-Products for a One Health Perspective.

Antimicrobial resistance is increasing globally and is now one of the major public health problems. Therefore, there is a need to search for new antimicrobial agents. The food industry generates large amounts of by-products that are rich in bioactive compounds, such as phenolic compounds, which are known to have several health benefits, including antioxidant and antimicrobial properties. Thus, we aimed to characterize the phenolic compounds present in pomegranate, quince, and persimmon by-products, as well as their antioxidant and antimicrobial activities. Phenolic compounds were extracted from pomegranate, quince, and persimmon leaves, seeds, and peels using a mixture of ethanol/water (80/20). The polyphenol profile of the extracts was determined by high-performance liquid chromatography. The antioxidant activity of the extracts was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC) methods. Antimicrobial susceptibility was evaluated using the Kirby-Bauer disk diffusion method. In general, leaves showed higher concentrations of phenolics than the peel and seeds of fruits. In total, 23 phenolic compounds were identified and quantified, with sanguiin and apigenin-3-O-galactoside being present in the highest concentrations. Leaf extracts of pomegranate showed higher antioxidant activities than the other components in all methods used. In general, all extracts had a greater antimicrobial activity against Gram-positive bacteria. Persimmon leaf and seed extracts inhibited a greater number of bacteria, both Gram-positive and -negative. The lowest minimum inhibitory concentration (MIC) detected among Gram-positive and -negative bacteria was 10 mg/mL for pomegranate peel and leaf extracts against Staphylococcus aureus and S. pseudintermedius and for pomegranate leaf extract against Escherichia coli. Our results reinforce the need to value food industry by-products that could be used as food preservatives and antibiotic adjuvants against multiresistant bacteria.

Epileptiform activity influences theta-burst induced LTP in the adult hippocampus: a role for synaptic lipid raft disruption in early metaplasticity?

Non-epileptic seizures are identified as a common epileptogenic trigger. Early metaplasticity following seizures may contribute to epileptogenesis by abnormally altering synaptic strength and homeostatic plasticity. We now studied how in vitro epileptiform activity (EA) triggers early changes in CA1 long-term potentiation (LTP) induced by theta-burst stimulation (TBS) in rat hippocampal slices and the involvement of lipid rafts in these early metaplasticity events. Two forms of EA were induced: (1) interictal-like EA evoked by Mg2+ withdrawal and K+ elevation to 6 mM in the superfusion medium or (2) ictal-like EA induced by bicuculline (10 µM). Both EA patterns induced and LTP-like effect on CA1 synaptic transmission prior to LTP induction. LTP induced 30 min post EA was impaired, an effect more pronounced after ictal-like EA. LTP recovered to control levels 60 min post interictal-like EA but was still impaired 60 min after ictal-like EA. The synaptic molecular events underlying this altered LTP were investigated 30 min post EA in synaptosomes isolated from these slices. EA enhanced AMPA GluA1 Ser831 phosphorylation but decreased Ser845 phosphorylation and the GluA1/GluA2 ratio. Flotillin-1 and caveolin-1 were markedly decreased concomitantly with a marked increase in gephyrin levels and a less prominent increase in PSD-95. Altogether, EA differentially influences hippocampal CA1 LTP thorough regulation of GluA1/GluA2 levels and AMPA GluA1 phosphorylation suggesting that altered LTP post-seizures is a relevant target for antiepileptogenic therapies. In addition, this metaplasticity is also associated with marked alterations in classic and synaptic lipid raft markers, suggesting these may also constitute promising targets in epileptogenesis prevention.

CONSUMO DE SUBSTÂNCIAS PSICOATIVAS POR PROFISSIONAIS MÉDICO S DE UM HOSPITAL DE ALTA COMPLEXIDADE / CONSUMPTION OF PSYCHOACTIVE SUBSTANCES BY MEDICAL PROFESSIONALS AT A HIGH COMPLEXITY HOSPITAL / CONSUMO DE SUSTANCIAS PSICOACTIVAS POR PROFESIONALES MÉDICOS EN UN HOSPITAL DE ALTA COMPLEJIDAD

Objetivo: analisar o consumo de substâncias psicoativas por médicos de um hospital de alta complexidade do estado do Piauí. Método: estudo transversal realizado com 90 profissionais. A coleta dos dados ocorreu entre dezembro de 2018 e julho de 2019, por meio de questionário semiestruturado, composto por variáveis sociodemográficas, ocupacionais e relacionadas à saúde e ao consumo de substâncias psicoativas, além dos instrumentos Alcohol, Smoking and Substance Involvement Screening Test e o Alcohol Use Disorders Identification Test. Os dados foram analisados por meio de estatística descritiva, odds ratio, teste de qui-quadrado e teste de correlação de Pearson. Resultados: Dentre as substâncias psicoativas mais consumidas, destacou-se o álcool (94%). Dentre as ilícitas, verificou-se o uso de maconha (97,6%) e inalantes (97,6%). Quanto às motivações para o consumo de substâncias psicoativas, o uso recreativo (57,7%) e para relaxamento (26,6%) foram mais prevalentes; em relação ao contexto, predominou o uso para descanso (48,8%). De modo geral, os participantes apresentaram baixo risco para o consumo de substâncias psicoativas. Conclusão: O comportamento de risco identificado direciona a atenção para demandas de cuidado em saúde mental. Assim, emerge a necessidade de fortalecer o círculo de proteção em saúde do trabalhador voltado ao enfrentamento da problemática

Aim: to analyze the consumption of psychoactive substances by physicians at a high complexity hospital in the state of Piauí. Method: cross-sectional study carried out with 90 professionals. Data collection took place between December 2018 and July 2019, through a semi -structured questionnaire, composed of sociodemographic, occupational and health -related variables and psychoactive substance consumption, as well the Alcohol, Smoking and Substance Involvement Screening Test and the Alcohol Use Disorders Identification Test. The data were analyzed using descriptive statistics, odds ratio, chisquare test and Pearson correlation test. Results: Among the most consumed psychoactive substances, the alcohol presented highlighted (94,0%). Among the illicit, we identified use of marijuana (97,6%) and inhalants (97,6%). Regarding the motivations for the consumption of psychoactive substances, recreational use (57,7%) and relaxation (26,6%) were more prevalent; as for the context, use for rest predominated (48.8%). In general, participants showed a low risk for the consumption of psychoactive substances. Conclusion: Identified risk behaviors direct attention to the demands of mental health care. Therefore, the need to strengthen the workers' health protection circle arises, focused on facing this problem

Objetivo: analizar el consumo de sustancias psicoactivas por médicos de un hospital de alta complejidad en el estado de Piauí. Método: estudio transversal realizado con 90 profesionales. La recolección de datos se realizó entre diciembre de 2018 y julio de 2019, a través de un cuestionario semiestructurado, compuesto por variables sociodemográficas, laborales, de salud y consumo de psicotrópicos, además del Alcohol, Smoking and Substance Involvement Screening Test y el Alcohol Use Disorders Identification Test. Los datos fueron analizados mediante estadística descriptiva, razón de momios, prueba de chicuadrado y correlación de Pearson. Resultados: Entre las sustancias psicoactivas más consumidas el alcohol mostró destacado (94,0%). Entre los ilícitos, estaba el uso de marihuana (97,6%) e inhalables (97,6%). Con respecto a las motivaciones para el consumo de sustancias psicoactivas, prevalecieron: uso recreativo (57,7%) y relajación (26,6%); en cuanto al contexto, predominó el uso para el descanso (48,8%). En general, los participantes mostraron un riesgo bajo para el consumo de sustancias psicoactivas. Conclusión: Las conductas de riesgo identificadas orientan la atención a las demandas de cuidado en salud mental. Por lo tanto, surge la necesidad de fortalecer el círculo de protección de la salud de los trabajadores, centrado en enfrentar esto.

Digital transformation: a review on artificial intelligence techniques in drilling and production applications.

The use of digital and artificial intelligence technologies has expanded and influenced business models and the opening of opportunities for the generation of value in several organizations, in a movement known as industry 4.0. The oil industry has been following the path of several other industrial sectors and has implemented digitalization to solve different challenges and problems. The present work, supported by extensive research carried out in the specialized literature, shows relevant applications of digital transformation to solve problems in drilling and production operations during the life cycle of an oil well. The main issues addressed in the research were stuck pipes and hydrate formation. The achievements show that control systems and the various sensors used during drilling and the useful life of an oil well generate data that creates opportunities for the use of computational and artificial intelligence techniques. New technologies associated with digital transformation include smart surveillance systems, real-time monitoring, and intelligent equipment. In a well oil environment, these novelties are associated with fault detection and prediction systems to avoid or reduce problems or accidents that may cause costs or, in extreme cases, lead to the loss of the well. The study also points out that the oil industries, research centers, and universities are increasingly working together to understand the challenges and overcome the problems associated with the implementation and greater use of digital transformation technologies.

What role do patients prefer in medical decision-making?: a population-based nationwide cross-sectional study.

OBJECTIVE: To assess patients' preferred roles in healthcare-related decision-making in a representative sample of the Portuguese population. DESIGN: Population-based nationwide cross-sectional study. SETTING AND PARTICIPANTS: A sample of Portuguese people 20 years or older were interviewed face-to-face using a questionnaire with the Problem-Solving Decision-Making scale. OUTCOMES: The primary outcome was patients' preferred role for each vignette of the problem-solving decision-making scale. Sociodemographic factors associated with the preferred roles were the secondary outcomes. RESULTS: 599 participants (20-99 years, 53.8% women) were interviewed. Three vignettes of the Problem-Solving Decision-Making scale were compared: morbidity, mortality and quality of life. Most patients preferred a passive role for both the problem-solving and decision-making components of the scale, particularly for the mortality vignette (66.1% in the analysis of the three vignettes), although comparatively more opted to share decision in the decision-making component. For the quality of life vignette, a higher percentage of patients wanted a shared role (44.3%) than with the other two vignettes. In the problem-solving component, preferences were significantly associated with area of residence (p<0.001) and educational level (p=0.013), while in the decision-making, component preferences were associated with age (p=0.020), educational level (p=0.015) and profession (p<0.001). CONCLUSIONS: In this representative sample of the Portuguese mainland population, most patients preferred a practitioner-controlling role for both the problem-solving and decision-making components. In a life-threatening situation, patients were more willing to let the doctor decide. In contrast, in a less serious situation, there is a greater willingness to participate in decision-making. We have found that shared decision-making is more acceptable to better-educated patients in the problem-solving component and to people who are younger, higher educated and employed, in the decision-making component.

Patients' Perspectives about Lifestyle Behaviors and Health in the Context of Family Medicine: A Cross-Sectional Study in Portugal.

Lifestyle interventions are recognized as essential in the prevention and treatment of non-communicable diseases. Previous studies have shown that Portuguese patients tend to give more importance to diagnostic and laboratory tests than to lifestyle measures, and seem unaware that behavioral risks are the main modifiable risk factors. The study aimed to analyze patients' perspectives about lifestyle behaviors and health in the context of family medicine in Portugal. A population-based cross-sectional study was carried out in Portugal (the mainland). A total of 900 Portuguese patients aged ≥20 years, representative of the population, were surveyed using face-to-face questionnaires. Participants were selected by the random route method. Descriptive statistics and non-parametric tests were performed to evaluate differences between the personal beliefs and the personal behavior self-assessment, as well as between the level of importance given to the family doctor to address health behaviors and the reported approach implemented by the family doctor, and its association with bio-demographic variables. The results indicate that the vast majority of this Portuguese cohort has informed beliefs regarding lifestyle behaviors, tends to overestimate their own behavior self-assessment, and strongly agrees that it is important that their family doctor asks/advises on these lifestyle behaviors, although the proportion of those who totally agree that their family doctor usually does this is significantly lower. Differences concerning bio-demographic variables were found. Future research directions should focus on the politics, economics, and policy aspects that may have an impact in this area. It will also be important to understand more broadly the relationships between lifestyle behaviors and clinical, physical, and sociodemographic variables.

Kaolin, Ascophyllum nodosum and salicylic acid mitigate effects of summer stress improving hazelnut quality.

BACKGROUND: Various strategies are needed to mitigate the negative impact on or to increase fruit quality. The effect of spraying kaolin (K), Ascophyllum nodosum (An) and salicylic acid (SA), in trees with and without irrigation, on quality and sensorial attributes of hazelnut (Grada de Viseu cultivar) was investigated during two consecutive years (2016 and 2017) in a commercial orchard located in Moimenta da Beira, Portugal. RESULTS: The treatments affected positively the biometric parameters nut and kernel weight, length, width, thickness and volume as well as the vitamin E level, antioxidant activity and content of some individual phenolics, such as protocatechuic acid, gallocatechin, catechin and epicatechin. The levels of amino acids in hazelnut kernels decreased in all the assayed treatments, while the kernel colour and sensorial attributes were not affected by the treatments. Hazelnut physical properties (nut and kernels), chemical and phytochemical composition and antioxidant activities were positively related. CONCLUSIONS: The application of K, An and SA improved the hazelnut tree response to climate change, without compromising the hazelnut chemical and sensorial quality. Furthermore, due to the similar observations for the same treatments with and without irrigation, it can be stated that K, An and SA can be efficient and cost-effective tools to mitigate summer stress in rain-fed orchards. © 2020 Society of Chemical Industry.

Kiwi fruit residues from industry processing: study for a maximum phenolic recovery yield.

A response surface methodology was used to study the conditions for a maximum recovery of phenolics from processing kiwi fruit residues. Ethanolic extracts were prepared with different conditions of pH (2, 5, 10), temperature (30, 50 and 70 ºC) and time (10, 20, 30 min). Total phenolics, total flavonoids content and antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl scavenging capacity and ferric reducing antioxidant power were determined. Samples from optimal extraction condition were injected HPLC-DAD system to access the phenolic profile and content. The best extraction conditions were pH solvent of 2, 70 ºC of temperature and 20 min of extraction. Ten phenolics were identified: caffeic acid and its derivatives, chlorogenic acid and ferulic acid, (+)-catechin, (-)-epicatechin), rutin and quercitrin. These phenolics often reported as having important antioxidant, anti-inflammatory, antiaging and anticancer activities, turn this residues and excellent source of bioactive compounds to be used in agro-food, cosmetics or phytochemical industries.

The Problem-Solving Decision-Making scale-translation and validation for the Portuguese language: a cross-sectional study.

OBJECTIVES: To translate and validate the Problem-Solving Decision-Making scale instrument into the Portuguese language. DESIGN: Cross-sectional study. SETTING PARTICIPANTS: The Problem-Solving Decision-Making scale was translated from English to Portuguese and then back-translated to obtain a final version. The questionnaire was then applied face-to-face from January to March 2019 in a representative sample of the Portuguese population (n=301 people aged 20 years or more) to validate the Problem-Solving Decision-Making scale in a Portuguese population. OUTCOMES: Principal component analysis and Cronbach's alpha. RESULTS: Principal component analysis was used to evaluate the validity of the internal structure of the scale. The results identified two components: problem-solving and decision-making with an explained variance of 65.9%. For internal consistency, three different techniques were used and applied to the two components. All of the items have very good internal consistency (problem-solving Cronbach's alpha=0.931 and decision-making Cronbach's alpha=0.951). CONCLUSIONS: The validation of the Portuguese scale agreed well with the existing literature. The scale can be divided into two components: the problem-solving component and the decision-making component. The translated scale demonstrated good internal consistency and can therefore be used in future studies.

Atuação docente em Educação Física escolar na Educação de Jovens e Adultos (EJA): ações de Promoção da Saúde

RESUMO O objetivo deste estudo foi conhecer como os professores de Educação Física vem desenvolvendo suas aulas em torno da temática de Promoção da Saúde na Educação de Jovens e Adultos (EJA), uma modalidade da educação básica brasileira. A pesquisa, de caráter qualitativo, adotou como estratégia a entrevista individual com 10 professores através de curso de extensão, e utilizou como instrumento de análise de dados o Discurso do Sujeito Coletivo. Os resultados apontam que o discurso biologicista continua hegemônico nas concepções dos professores de Educação Física, possuindo um peso considerável nas reflexões de saúde e ser saudável, assim como, nas abordagens com esta temática na EJA.

ABSTRACT The aim of this study was to know how the Physical Education teachers, have been developing their classes around the theme of Health Promotion in the context of Youth and Adult Education, a form of Brazilian basic education. The research of qualitative approach, adopted as strategy ten individual interviews with teachers from the extension course, and used as a tool of analysis the technical discourse of the collective subject. The results presented point out that the biological discourse remains hegemonic in the conceptions of Health Promotion of Physical Education teachers, having a considerable weight in reflections on health and being healthy, as well as in the teaching approaches with this theme in this modality of education.

RESUMEN El objetivo de este estudio fue conocer cómo los profesores de Educación Física vienen desarrollando sus clases en torno a la temática de Promoción de la Salud en la Educación de Jóvenes y Adultos (EJA), una modalidad de la educación básica brasileña. La investigación, de carácter cualitativo, adoptó como estrategia la entrevista individual con 10 profesores a través del curso de extensión ofrecido, y utilizó la técnica del Discurso del Sujeto Colectivo como instrumento de análisis de datos. Los resultados apuntan que el discurso biologicista continúa hegemónico en las concepciones de los profesores de Educación Física, teniendo un peso considerable en las reflexiones de salud y ser sano, así como en los abordajes con esta temática en la EJA.

Formação de professores em educação física e a educação de jovens e adultos / Physical education teacher training and youth and adult education / Formación de profesores en educación física y educación de jóvenes y adultos

O artigo objetiva compartilhar estudos referentes à formação inicial de professores e professoras de Educação Física para atuação na Educação de Jovens e Adultos (EJA), através de ações acadêmicas desenvolvidas em duas universidades públicas federais. A discussão apresentada se origina das pesquisas dessas realidades que, a partir de distintas bases empíricas, considerando a educação de jovens e adultos como direito e devir e apoiadas em visão crítica da Educação Física, analisam esse fenômeno em dois recortes espaciais, no Sul e no Sudeste do Brasil. Nas considerações, aponta a importância e a responsabilidade das universidades para a concretização dessa prática pedagógica em uma modalidade que é expressão das desigualdades sociais e, complementarmente a isso, o necessário compromisso com uma formação de professores de Educação Física atenta às especificidades da EJA

The article aims to share studies about initial training of Physical Education teachers to work with youth and adult education, through academic actions developed in two Federal Universities. The discussion originates from studies on these realities that, based on distinct empirical foundations and considering the education of youth and adults as a right and becoming, based on a critical view of Physical Education, analyze this phenomenon in two spaces ­ Brazil's South and Southeast regions. The considerations point out Universities' importance and responsibility for making this pedagogical practice come through in a way that expresses social inequalities. The necessary commitment to training of Physical Education teachers that takes into account the specificities of youth and adult education complements that

El artículo tiene por objetivo compartir estudios referentes a la formación inicial de profesores y profesoras de Educación Física para ejercer en la Educación de Jóvenes y Adultos, a través de acciones académicas desarrolladas en dos Universidades Públicas Federales. La discusión presentada se origina en investigaciones sobre esas realidades que, desde distintas bases empíricas y considerando la educación de jóvenes y adultos como derecho y devenir y, apoyadas en una visión crítica de la Educación Física, analizan ese fenómeno en dos recortes espaciales: en el sur y en el sudeste de Brasil. En las consideraciones, se apunta la importancia y la responsabilidad de las Universidades para la concreción de esa práctica pedagógica en una modalidad que es expresión de las desigualdades sociales y, complementario a ello, el necesario compromiso con una formación de profesores de Educación Física atenta a las especificidades de la Educación de Jóvenes y Adultos

Residências terapêuticas: a percepção de moradores acerca de autonomia, relacionamentos e contratualidade / Therapeutic residences: residents' perception of autonomy, relationships and contractuality

Objetivo: Analisar a vivência dos moradores de uma residência terapêutica no município de Teresina. Metodologia: Estudo descritivo com abordagem qualitativa, realizado em residência terapêutica com cinco moradores que ali viviam por no mínimo 6 meses, por meio de entrevista semiestruturada, que através da análise de conteúdo segundo Minayo gerou três categorias: os moradores e relacionamento com seus pares e comunidade; os moradores e o poder de contratualidade; os moradores e sua autonomia. Resultados: Os moradores relataram que recebem bons cuidados e sentem-se acolhidos, além de boa convivência com os cuidadores. Em relação à comunidade, houve inicialmente resistência, contudo, a dificuldade foi superada. Quanto à autonomia e contratualidade, a negociação é quase incipiente. Em seu cotidiano há horários préestabelecidos para atividades cotidianas e próprias da casa que são determinadas pela responsável da Residência Terapêutica. Sobre o benefício recebido do Governo Federal, há permissão dos moradores para a coordenadora da residência administrar seus pecunhos. Conclusão: Sugere-se formulação ou reformulação da dinâmica da moradia, capacitação dos cuidadores, permanente avaliação da autonomia dos moradores possível para cada caso e um maior trabalho para que, de fato, a reinserção social se concretize.

Objective: To analyze the experience of residents of a therapeutic residence in the municipality of Teresina. Methodology: it is a descriptive study with qualitative approach, conducted in therapeutic residence with five residents who lived there for at least 6 months, through semi-structured interview using Minayo's content analysis which generated three categories: residents and relationship with their peers and community; residents and the power of contractuality; the residents and their autonomy. Results: Residents reported that they receive good care and feel welcomed, as well as having good contact with caregivers. Regarding the community, there was initially resistance, however, the difficulty was overcome. About autonomy and contractuality, negotiation is almost incipient. In your daily life there are pre-established times for daily activities and the house that are determined by the head of the Therapeutic Residence. Regarding the benefit received from the Federal Government, residents are allowed to the residence coordinator to administer their pecuniary. Conclusion: It is suggested the formulation or reformulation of housing dynamics, caregivers training, permanent assessment of the residents' autonomy possible for each case and a greater work to actually bring about social reintegration.

Relato de caso: diagnóstico de síndrome coronariana aguda sem supra ST em paciente adulto com ventrículo único e transposição de grandes artérias / Acute coronary syndrome diagnosis in an adult with single ventricle and transposition of the great arteries: case report

As cardiopatias congênitas se apresentam em 3,7 a 8 a cada 1000 nascidos vivos 5. A transposição das grandes artérias (5% a 7% de todas as cardiopatias) é caracterizada por uma inversão na topografia da artéria aorta com a artéria pulmonar, tornando necessária a mistura do sangue venoso e arterial, realizado cirurgicamente ou não, para que o indivíduo consiga sobreviver. Já o ventrículo único representa 3,8% de todas as cardiopatias congênitas¹. Caracteriza-se quando uma única cavidade ventricular recebe duas valvas atrioventriculares ou uma valva atrioventricular comum. O objetivo desse estudo é descrever um caso onde estão presentes as duas cardiopatias simultâneas e qual foi o seguimento clínico do paciente

Traposition of the great arteries is characterized by an inversion on the topography of aorta artery and pulmonary artery. It is a condition incompatible with life, so it is necessary to combine the arterial blood and venous blood surgically or not. The single ventricle means that there is only one ventricle cavity and it recives two atrioventricular valves or a unique valve. Those represent 5% to 7% and 3,8% of all congenital heart diseases respectively. Objective: Describe a case which contains both congenital heart diseases simultaneously e the clinical follow ­ up of the patient

Estigma e preconceito relacionados à pessoa com transtorno mental / Stigma and preconcept related to the person with mental disorder

Objetivo: relatar a experiência de profissionais de enfermagem no atendimento a pessoas com transtornos mentais que vivenciam estigma e preconceito por tal condição. Metodologia: trata-se de um relato de experiência produzido no período de fevereiro a junho de 2018 por estudantes e profissionais de enfermagem, a partir de vivências nas consultas de enfermagem realizadas com usuários de um hospital psiquiátrico de Teresina-PI. Resultados: a partir da experiência surgiu o questionamento de como os pacientes enfrentavam as críticas e preconceitos em seu dia a dia. Com isso procurou-se mostrar a importância do enfermeiro na reinserção desse paciente na sociedade, ajudar a família a compreender esses indivíduos, como também buscar formas de divulgar o assunto para que as pessoas possam compreendam mais estes, e diminuam as críticas. Conclusão: o profissional enfermeiro tem um papel importante na sensibilização, e no cuidado deste cliente, atuando tanto na prevenção da doença física, quanto no adoecimento mental.

Objective: to report the experience of nursing professionals in the care of people with mental disorders who experience stigma and prejudice for such condition. Methodology: this is an experience report produced in the period from February to June 2018 by students and nursing professionals, based on experiences in nursing consultations performed with users of a psychiatric hospital in Teresina-PI. Results: from the experience came the questioning of how patients faced the criticisms and prejudices in their day to day. The aim of this study was to show the importance of the nurse in the reinsertion of this patient in the society, to help the family to understand these individuals, as well as to find ways to spread the subject so that people can understand these more, and reduce criticism. Conclusion: the nurse professional plays an important role in the sensitization and care of this client, acting both in the prevention of physical illness and in mental illness.

Identification of a Novel Mutation in a Family with Pseudohypoparathyroidism Type 1a.

INTRODUCTION: Pseudohypoparathyroidism type 1a is caused by GNAS mutations leading to target organ resistance to multiple hormones rather than parathyroid hormone, resulting not only in hypocalcemia, but also in Albright's hereditary osteodystrophy phenotype. MATERIALS AND METHODS: DNA sequencing of the GNAS gene identified a novel heterozygous mutation in peripheral blood leukocytes in the family presented in this case report. RESULTS: We present a case of a 25-year-old woman with pseudohypoparathyroidism type 1a admitted with seizures, whose family presents an autosomal dominant transmission of a novel heterozygous GNAS mutation (c.524_530+3del). CONCLUSION: Pseudohypoparathyroidism type 1a is mostly caused by inactivating GNAS mutations that have been gradually reported in the literature that lead to a typical and complex clinical phenotype and resistance to multiple hormones. The deletion caused by the mutation identified in the presented case has not been reported previously.

Profiling of Polyphenol Composition and Antiradical Capacity of Erica cinerea.

The aim of the current study was to determine the profile and content of polyphenols present in Erica cinerea, an important plant species from Northern Portuguese flora and often reported as having anti-inflammatory, antioxidant, and anti-radical activity. The analysis of polyphenols was performed by HPLC-DAD/UV-Vis, and the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) method was used to evaluate its radical scavenging activity. HPLC analysis showed that both plants presented a great diversity of compounds, with 33% flavones, 28% flavanols, and 26% hydroxycinnamic acids. The antiradical activity was dose-dependent, and the IC50 values were 0.251 mg mL-1. Based on our study, E. cinerea presented interesting bioactive compounds and it can be used to extract and purify bioactive polyphenols to be used in pharmaceutical or agro-food industries.

Effects of Therapeutic Touch on Healing of the Skin in Rats.

CONTEXT: Therapeutic touch is a complementary treatment directed toward the balance of the energy field surrounding living beings. OBJECTIVE: This study's aim was to investigate the effect of therapeutic touch on wound area contraction and fibroblast proliferation in rat skin. DESIGN: This study was conducted using 24 male Wistar rats with dorsal wounds of diameter 8mm. The rats were divided into the following two groups: a control group: in this, the wounds were sanitized with filtered water and neutral-pH soap and a treatment group: in this, the wounds were sanitized as in the control group but the rats also underwent to daily sessions of therapeutic touch. Wound area was measured on days 1, 4, and 7 using imagelab software, version 2.4 R.C. On days 4 and 7, six animals in each group were euthanized so that the lesioned tissue could be collected for fibroblast counts and histological evaluations. RESULTS: On days 1 and 4, wound areas were similar in both groups. Moreover, no significant differences in fibroblast counts were observed on day 4. On day 7, however, fibroblast counts were significantly higher in the treated group than in the control group, with a subsequent wound shrinkage. CONCLUSION: These data indicate that therapeutic touch may accelerate wound repair, possibly by increasing fibroblast activity.

Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection.

Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties. They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives. The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis. Method validation controls were performed and recovery experiments were assayed. The data was statically treated and compared with published works. The current method allowed a feasible identification of indole glucosinolates and it was possible to identify accurately three indole glucosinolate compounds (glucobrassicin, 4-methoxyglucobrassicin, and 1-methoxyglucobrassicin) in roots of Chinese cabbage. The method demonstrated a good linearity (R2 > 0.99), a good precision, and selectivity sensitivity. In conclusion, this protocol provides an accessible method to extract and quantify glucosinolates in plant samples. Thus, based on our results, the method is valid and can be extended to other plant or food matrices.