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1.
Microbes Infect ; 8(2): 420-5, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16242372

ABSTRACT

Activated phagocytes oxidize the hormone melatonin to N1-acethyl-N2-formyl-5-methoxykynuramine (AFMK) in a superoxide anion- and myeloperoxidase-dependent reaction. We examined the effect of melatonin, AFMK and its deformylated-product N-acetyl-5-methoxykynuramine (AMK) on the phagocytosis, the microbicidal activity and the production of hypochlorous acid by neutrophils. Neither neutrophil and bacteria viability nor phagocytosis were affected by melatonin, AFMK or AMK. However these compounds affected the killing of Staphylococcus aureus. After 60 min of incubation, the percentage of viable bacteria inside the neutrophil increased to 76% in the presence of 1 mM of melatonin, 34% in the presence of AFMK and 73% in the presence of AMK. The sole inhibition of HOCl formation, expected in the presence of myeloperoxidase substrates, was not sufficient to explain the inhibition of the killing activity. Melatonin caused an almost complete inhibition of HOCl formation at concentrations of up to 0.05 mM. Although less effective, AMK also inhibited the formation of HOCl. However, AFMK had no effect on the production of HOCl. These findings corroborate the present view that the killing activity of neutrophils is a complex phenomenon, which involves more than just the production of reactive oxygen species. Furthermore, the action of melatonin and its oxidation products include additional activities beyond their antioxidant property. The impairment of the neutrophils' microbicidal activity caused by melatonin and its oxidation products may have important clinical implications, especially in those cases in which melatonin is pharmacologically administered in patients with infections.


Subject(s)
Kynuramine/analogs & derivatives , Kynuramine/pharmacology , Melatonin/metabolism , Neutrophils/drug effects , Neutrophils/immunology , Cells, Cultured , Humans , Hypochlorous Acid/metabolism , Kynuramine/chemistry , Kynuramine/metabolism , Neutrophil Activation/immunology , Neutrophils/microbiology , Neutrophils/physiology , Oxidation-Reduction , Phagocytosis , Staphylococcus aureus/immunology
2.
Semina ; 18(Ed.esp): 79-84, nov. 1997. graf
Article in Portuguese | LILACS | ID: lil-223976

ABSTRACT

O Grupo Interdisciplinar e Multiprofissional n§ 11 (GIM 11), após entrar em contato e conhecer a realidade da comunidade do Jardim Bandeirantes, em Londrina, desenvolveu um trabalho de orientaçäo sobre sexualidade no Colégio Estadual Antônio Moraes de Barros, entre os alunos matriculados a partir da 5ª série. O principal objetivo do grupo era transmitir aos estudantes informaçöes básicas sobre sexualidade e suas implicaçöes, proporcionando aos mesmos a formaçäo de uma visäo crítica sobre o assunto. Outros objetivos importantes foram a composiçäo de uma equipe multiprofissional e integrada e a formaçäo de uma consciência ética e social entre os membros do grupo, surgindo daí o acadêmico com o perfil que levará ao novo modelo do profissional de saúde de que a sociedade necessita


Subject(s)
Health Education
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