Study of chemical constituents, antioxidants and antimicrobial activities of Tamarindus indica L. seed.

The fruits of Tamarindus indica L. are consumed worldwide, with various parts of the plant being used for medicinal purposes. The residues (pericarp and seeds) generated during cellulose processing are of significant value as they contain bioactive compounds with diverse biological activities. The objective of this study was to evaluate the chemical constituents of the ethyl acetate fraction as possible substitutes for synthetic compounds with biological properties using ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) analysis and the evaluation of the antioxidant activity (ferric reducing antioxidant power [FRAP], 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid [ABTS], and 1-diphenyl-2-picrylhydrazyl [DPPH]), total phenolic compounds (TPC), and antimicrobial activity of the hydroalcoholic extract and tamarind seed fractions were also performed. The chemical investigation of the acetate fraction using UHPLC-HRMS/MS resulted in the putative identification of 14 compounds, including flavonoids, (+)-catechin/(-)-epicatechin, procyanidin B2, procyanidin C2, isoquercetin, quercetin, luteolin, rutin, taxifolin, eriodictyol, kaempferide, hydroxybenzoic acid, protocathecuic acid, and protocathecuic acid methyl and ethyl esters derivatives. The crude hydroalcoholic extract exhibited the best results in terms of TPC: 883.87 gallic acid equivalent (GAE; mg/g) and antioxidant activity: FRAP: 183.29 GAE (mg/g), ABTS: 39.67%, and DPPH: 91.08%. The extract exhibited excellent antibacterial activity against gram-positive bacteria, specifically Staphylococcus aureus minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC; 62.5/125 g/mL) and Bacillus cereus MIC/MBC (125/250 g/mL), and gram-negative bacteria, specifically Aeromonas hydrophila MIC/MBC (125/250 µg/mL) and Pseudomonas aeruginosa MIC/MBC (250/500 g/mL). Morphological damage to cells was observed using flow cytometry and scanning electron microscopy. Tamarind seeds contain unique bioactive compounds that should be explored for their use as novel food preservatives. PRACTICAL APPLICATION: Original data were obtained regarding the Tamarindus indica L. seed extract and the ethyl acetate and hexane fractions. This research aimed to investigate the potential of these for food preservation and as alternatives to additives and synthetic compounds added to cattle feed. This paper reports novel findings regarding the chemical composition of the extract and its antioxidant activity, along with its antimicrobial activity against bacteria (gram-positive: Staphylococcus aureus, Bacillus cereus, and gram-negative: Salmonella enterica serovar Enteritidis, Escherichia coli, Pseudomonas aeruginosa, and Aeromonas hydrophila) and yeasts (Candida albicans and Saccharomyces cerevisiae).

Ingestive behavior of Girolando steers supplemented on tropical pastures.

In this study, we evaluated the feeding behavior of Girolando steers on Brachiaria brizantha cultivar Marandu pastures. Twenty-two animals with an average initial weight of 209.09 ± 8.18 kg were distributed in a completely randomized design in a 3 × 2 factorial arrangement (three seasons and two nutritional plans (NP)) with 11 replicates, as follows: rainy season 1 (NP1, mineral mixture ad libitum, and NP2, nitrogen/energy supplement [2 g.kg‒1 body weight [BW]]); dry season (NP1, nitrogen/energy supplement [1 g.kg‒1 BW], and NP2, nitrogen/energy supplement [2 g.kg‒1 BW]); and rainy season 2 (NP1, mineral mixture ad libitum, and NP2, nitrogen/energy supplement ([1 g.kg‒1 BW]). Total chewing time was longer in NP1 (566.44 vs 528.33 min.day‒1 in NP2) (p < 0.05). The grazing, idle, trough, and total chewing times were affected by the interaction between nutritional plans and seasons. The period expended grazing was longer for the NP1. The idle time was affected by the nutritional plans and was lower for the NP1. Feeding at the trough was not affected by the nutritional plans. The animals of the NP2 showed the highest feed efficiencies in DM and NDF (0.91 and 0.52 vs 0.75 and 0.45 in NP1, respectively). Rainy season 2 had the highest efficiencies. The feeding behavior changes according to the supplementation level. Nutritional plan 2 in the second rainy season presented the best results.

Carcass characteristics and meat evaluation of cattle finished in temperate pasture and supplemented with natural additive containing clove, cashew oil, castor oils, and a microencapsulated blend of eugenol, thymol, and vanillin.

BACKGROUND: Forty crossbred steers were supplemented with different doses (from 0 control to 6000 mg/animal/day) of natural additive blend containing clove essential oil, cashew oil, castor oil, and a microencapsulated blend of eugenol, thymol, and vanillin for 80 days. Carcass characteristics, drip loss, and antioxidant activity were evaluated 24 h post mortem on longissimus thoracis, and the effects of aging (until 14 days) were evaluated for water losses (thawing/aging and cooking), texture, color, and lipid oxidation. RESULTS: The use of the natural additive blend did not modify (P > 0.05) carcass characteristics but did, however, modify body composition (P < 0.05). Drip losses were unaffected by the treatments tested (P > 0.05). There was an observed quadratic effect (P < 0.05) on losses from thawing/aging on the first day of storage. Regarding the effects of natural additives on cooking losses, there was a quadratic effect (P < 0.05) among the treatments on day 7 of aging. Differences between days of aging were only observed with control treatment. Shear force was similar among treatments on days 1 and 7 of aging. On day 14 a linear effect (P < 0.05) was observed. Also, a linear effect (P < 0.05) appeared on meat lightness, meat from the control group being clearer on day 1. No changes were observed in redness among treatments or days of storage (P > 0.05). Yellowness was not modified by the treatments (P > 0.05)but only by the days of storage in control and the lowest dosage used. CONCLUSION: The blend of natural additives has potential use in pasture feeding and could improve meat quality. However, doses should be adjusted. © 2021 Society of Chemical Industry.

Replacing sorghum with palm kernel cake in the diet decreased intake without altering crossbred cattle performance.

Palm kernel cake is a viable alternative for use in cattle feed to reduce production costs. The aim of this study was to evaluate the effects of concentrations in diet dry matter (DM) of palm kernel cake on intake and digestibility of nutrients and performance of cattle finished in a feedlot system. Forty non-castrated crossbred cattle, with an average age of 24 ± 2 months and an average weight of 331.12 ± 36.20 kg, were distributed among four treatments (control 0%, without heart of palm kernel cake in the diet; 8%, 16%, and 24% of palm kernel cake in diet DM). Variance and regression analysis were performed in function of the palm kernel cake concentrations in diet DM (P < 0.05). The dry matter intake decreased linearly (P < 0.05) as the concentrations in diet DM of palm kernel cake in the diet increased. The inclusion of palm kernel cake in the animals' diet decreased (P < 0.05) on dry matter digestibility. No effects (P > 0.05) of palm kernel cake on average daily gain or feed conversion were observed. The palm kernel cake can be included in the diet of crossbred bulls finished in confinement at concentrations in diet DM of up to 24%, as it has not influenced performance.

Increasing levels of supplementation for crossbred steers on pasture during the dry period of the year.

The aim of this study was to evaluate the effect of increasing concentrate supplementation levels on the intake, nutrient digestibility, and performance of crossbred steers during the dry period of the year. The experiment was developed on Princesa do Mateiro farm, in the municipality of Ribeirão do Largo, located in the southwest region of Bahia State, Brazil. Forty uncastrated male crossbred (½ Holstein-Zebu) steers with an average body weight (BW) of 232.55 ± 24.97 kg were distributed into four treatments in a completely randomized design with ten replicates. The animals were managed in an experimental area formed by Brachiaria brizantha cv. Marandu, in an intermittent grazing system. Treatments consisted of the following supplementation levels: 0.2% BW, with 60% crude protein (CP); 0.3% BW, with 40% CP; 0.4% BW, with 30% CP; and 0.5% BW, with 24% CP. The intakes of forage dry matter in kg/day and %BW and neutral detergent fiber corrected for ash and protein (NDFap) in %BW decreased linearly, whereas the intake of non-fibrous carbohydrates corrected for ash and protein in kg/day and average daily gain increased linearly. Therefore, the use of supplementation at 0.5% BW (24% crude protein) to provide gains of up to 0.500 kg/day is recommended for grazing steers during the post-weaning period in the dry season of the year.

Impact of increasing levels of spineless-cactus meal on the ingestive behaviour of grazing steers / Impacto do aumento dos níveis de farelo de palma forrageira sobre o comportamento ingestivo de novilhos em pastejo

The objective of this study was to evaluate the effect of different levels of spineless-cactus meal substituting for maize in diets for supplemented steers grazing on Urochoa brizantha during the postweaning phase. The experiment was conducted on Princesa do Mateiro farm, located in Ribeirão do Largo-BA, Brazil. Forty crossbred steers with an average body weight of 261 ± 7.46 kg were distributed into four groups for evaluation of the following four levels of substitution of ground maize for spinelesscactus meal: 0.00, 30.00, 60.00, and 90.00%. The results were analysed statistically by variance and regression analyses at a 5% error probability. Increasing the amount of spineless-cactus meal in the diet had a quadratic effect on the grazing time and on the time spent on other activities. The diet had a quadratic effect on the number of grazing periods, the number of periods at the trough, and the total feeding and chewing times. In contrast, the number of periods spent on other activities and on rumination was not influenced by the level of spineless-cactus meal. The bite rate, number of bites per swallow, and number of bites per day increased linearly, whereas the swallowing time and number of cuds ruminated per day decreased as the level of spineless-cactus meal added to the diet wasincreased. The feed and rumination efficiencies of dry matter, neutral detergent fibre, crude protein, and non-fibre carbohydrates were not influenced by the level of substitution of spineless-cactus meal for ground maize. Spinelesscactus meal levels close to 60% probably provided greater fibre degradation, leading the animals tospend more time on social interactions with the group, use the feed better, and possibly have a better feed conversion.

Objetivou-se avaliar os efeitos de diferentes níveis de substituição do milho pelo farelo de palma forrageira na dieta de novilhos mestiços sob suplementação em pastagens de Urochoa brizantha na fase de recria. O experimento foi conduzido na fazenda Princesa do Mateiro, no município de Ribeirão do Largo, Bahia. Foram utilizados 40 novilhos mestiços com peso corporal médio de 261 ± 7,46 kg, distribuídos em quatro grupos, para avaliação de quatro níveis de substituição do milho moído pelo farelo de palma forrageira, 0,00; 30,00; 60,00; e 90,00%. Os resultados foram analisados estatisticamente por meio de análise de variância e de regressão, a 5% de probabilidade de erro. O aumento da quantidade de farelo de palma na dieta provocou efeito quadrático no tempo de pastejo e no tempo destinado a outras atividades. Houve efeito quadrático da dieta sobre o número de períodos de pastejo, o número de períodos de cocho e para os tempos totais de alimentação e mastigação, ao contrário dos números de períodos destinados a outras atividades e à ruminação não foram influenciados pelos níveis de farelo de palma. A taxa de bocados, o número de bocados por deglutição e o número de bocados por dia aumentaram de forma crescente, enquanto o tempo de deglutição e o número de bolos ruminados por dia decresceram com o aumento dos níveis de farelo palma forrageira dieta. As eficiências de alimentação e ruminação da matéria seca, fibra em detergente neutro, proteína bruta e carboidratos não-fibrosos não foram influenciadas pelos níveis de substituição do milho moído pelo farelo de palma. Níveis próximos a 60% de farelo de palma forrageira provavelmente promoveu maior degradação da fibra, proporcionando aos animais mais tempo para a interação social entre o grupo, ummelhor aproveitamento e possivelmente melhor conversão alimentar.

Impact of increasing levels of spineless-cactus meal on the ingestive behaviour of grazing steers / Impacto do aumento dos níveis de farelo de palma forrageira sobre o comportamento ingestivo de novilhos em pastejo

The objective of this study was to evaluate the effect of different levels of spineless-cactus meal substituting for maize in diets for supplemented steers grazing on Urochoa brizantha during the postweaning phase. The experiment was conducted on Princesa do Mateiro farm, located in Ribeirão do Largo-BA, Brazil. Forty crossbred steers with an average body weight of 261 ± 7.46 kg were distributed into four groups for evaluation of the following four levels of substitution of ground maize for spinelesscactus meal: 0.00, 30.00, 60.00, and 90.00%. The results were analysed statistically by variance and regression analyses at a 5% error probability. Increasing the amount of spineless-cactus meal in the diet had a quadratic effect on the grazing time and on the time spent on other activities. The diet had a quadratic effect on the number of grazing periods, the number of periods at the trough, and the total feeding and chewing times. In contrast, the number of periods spent on other activities and on rumination was not influenced by the level of spineless-cactus meal. The bite rate, number of bites per swallow, and number of bites per day increased linearly, whereas the swallowing time and number of cuds ruminated per day decreased as the level of spineless-cactus meal added to the diet wasincreased. The feed and rumination efficiencies of dry matter, neutral detergent fibre, crude protein, and non-fibre carbohydrates were not influenced by the level of substitution of spineless-cactus meal for ground maize. Spinelesscactus meal levels close to 60% probably provided greater fibre degradation, leading the animals tospend more time on social interactions with the group, use the feed better, and possibly have a better feed conversion.(AU)

Objetivou-se avaliar os efeitos de diferentes níveis de substituição do milho pelo farelo de palma forrageira na dieta de novilhos mestiços sob suplementação em pastagens de Urochoa brizantha na fase de recria. O experimento foi conduzido na fazenda Princesa do Mateiro, no município de Ribeirão do Largo, Bahia. Foram utilizados 40 novilhos mestiços com peso corporal médio de 261 ± 7,46 kg, distribuídos em quatro grupos, para avaliação de quatro níveis de substituição do milho moído pelo farelo de palma forrageira, 0,00; 30,00; 60,00; e 90,00%. Os resultados foram analisados estatisticamente por meio de análise de variância e de regressão, a 5% de probabilidade de erro. O aumento da quantidade de farelo de palma na dieta provocou efeito quadrático no tempo de pastejo e no tempo destinado a outras atividades. Houve efeito quadrático da dieta sobre o número de períodos de pastejo, o número de períodos de cocho e para os tempos totais de alimentação e mastigação, ao contrário dos números de períodos destinados a outras atividades e à ruminação não foram influenciados pelos níveis de farelo de palma. A taxa de bocados, o número de bocados por deglutição e o número de bocados por dia aumentaram de forma crescente, enquanto o tempo de deglutição e o número de bolos ruminados por dia decresceram com o aumento dos níveis de farelo palma forrageira dieta. As eficiências de alimentação e ruminação da matéria seca, fibra em detergente neutro, proteína bruta e carboidratos não-fibrosos não foram influenciadas pelos níveis de substituição do milho moído pelo farelo de palma. Níveis próximos a 60% de farelo de palma forrageira provavelmente promoveu maior degradação da fibra, proporcionando aos animais mais tempo para a interação social entre o grupo, ummelhor aproveitamento e possivelmente melhor conversão alimentar.(AU)

Correlation between performance and ingestive behavior of steers post-weaned on pastures / Correlação entre o desempenho e o comportamento ingestivo de novilhos recriados em pastagens

The objective of this study was to evaluate the correlations existing between nutrient intake and ingestive behavior of crossbred steers in the post-weaning phase on Brachiaria brizantha cv. Marandu, during the rainy period. Twenty-two steers were distributed into a completely randomized design, with two types of supplement and eleven replications, managed under rotational grazing, in a 6.5 ha area. Pearsons Linear Correlation analysis was performed between the behavior variables and the performance variables values. The correlation coefficients were tested by the t test. The average daily gain (ADG) showed positive correlation with the times spent ruminating and eating at the trough and total chewing time. Positive correlation was observed between the idle time and feed conversion (FC). The bite rate and the number of bites per day presented positive correlations with ADG. Positive correlations were found between the variables rumination efficiency of dry matter (DM) and of neutral detergent fiber (NDF) with ADG. The ingestive behavior has a direct influence on the productive performance of crossbred steers post-weaned on pasture during the rainy period. Rumination efficiency improved when the animals were fed protein supplementation, which led to better utilization the ingested feed.(AU)

O objetivo deste estudo foi avaliar as correlações existentes entre a ingestão de nutrientes e comportamento ingestivo de novilhos mestiços na fase pós-desmame em pastagem de Brachiaria brizantha cv. Marandu, durante o período chuvoso. Vinte e dois animais foram distribuídos em um delineamento experimental inteiramente casualizado, com dois tipos de suplemento e onze repetições, administrados sob pastejo rotacionado, em uma área de 6,5 ha. A análise de correlação linear de Pearson foi realizado entre as variáveis de comportamento e os valores das variáveis de desempenho. Os coeficientes de correlação foram testados pelo teste de t. O ganho de peso médio diário (GMD) apresentou correlação positiva com os tempos passados em ruminação, comendo no cocho e tempo de mastigação total. Foi observada uma correlação positiva entre o tempo ocioso e conversão alimentar (CA). A taxa de bocados e o número de bocados por dia apresentaram correlações positivas com GMD. Foram encontradas correlações positivas entre as variáveis eficiência ruminação da matéria seca (MS) e da fibra em detergente neutro (FDN) com GMD. O comportamento ingestivo tem uma influência direta sobre o desempenho produtivo de novilhos mestiços desmamados em pasto durante o período chuvoso. A eficiência de ruminação melhorou quando os animais foram alimentados com suplementação proteica, o que levou a uma melhor utilização do alimento ingerido.(AU)

Correlation between performance and ingestive behavior of steers post-weaned on pastures / Correlação entre o desempenho e o comportamento ingestivo de novilhos recriados em pastagens

The objective of this study was to evaluate the correlations existing between nutrient intake and ingestive behavior of crossbred steers in the post-weaning phase on Brachiaria brizantha cv. Marandu, during the rainy period. Twenty-two steers were distributed into a completely randomized design, with two types of supplement and eleven replications, managed under rotational grazing, in a 6.5 ha area. Pearson’s Linear Correlation analysis was performed between the behavior variables and the performance variables values. The correlation coefficients were tested by the t test. The average daily gain (ADG) showed positive correlation with the times spent ruminating and eating at the trough and total chewing time. Positive correlation was observed between the idle time and feed conversion (FC). The bite rate and the number of bites per day presented positive correlations with ADG. Positive correlations were found between the variables rumination efficiency of dry matter (DM) and of neutral detergent fiber (NDF) with ADG. The ingestive behavior has a direct influence on the productive performance of crossbred steers post-weaned on pasture during the rainy period. Rumination efficiency improved when the animals were fed protein supplementation, which led to better utilization the ingested feed.

O objetivo deste estudo foi avaliar as correlações existentes entre a ingestão de nutrientes e comportamento ingestivo de novilhos mestiços na fase pós-desmame em pastagem de Brachiaria brizantha cv. Marandu, durante o período chuvoso. Vinte e dois animais foram distribuídos em um delineamento experimental inteiramente casualizado, com dois tipos de suplemento e onze repetições, administrados sob pastejo rotacionado, em uma área de 6,5 ha. A análise de correlação linear de Pearson foi realizado entre as variáveis de comportamento e os valores das variáveis de desempenho. Os coeficientes de correlação foram testados pelo teste de t. O ganho de peso médio diário (GMD) apresentou correlação positiva com os tempos passados em ruminação, comendo no cocho e tempo de mastigação total. Foi observada uma correlação positiva entre o tempo ocioso e conversão alimentar (CA). A taxa de bocados e o número de bocados por dia apresentaram correlações positivas com GMD. Foram encontradas correlações positivas entre as variáveis eficiência ruminação da matéria seca (MS) e da fibra em detergente neutro (FDN) com GMD. O comportamento ingestivo tem uma influência direta sobre o desempenho produtivo de novilhos mestiços desmamados em pasto durante o período chuvoso. A eficiência de ruminação melhorou quando os animais foram alimentados com suplementação proteica, o que levou a uma melhor utilização do alimento ingerido.