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1.
Braz J Infect Dis ; 14(3): 242-51, 2010.
Article in English | MEDLINE | ID: mdl-20835507

ABSTRACT

The main objectives of the present study were to investigate the clinical and laboratory features of meningococcal disease in the city of Rio de Janeiro, Brazil, during the overlap of 2 epidemics in the 1990s. We conducted a study of a series of cases of meningococcal disease admitted in a Meningitis Reference Hospital. All clinical isolates available were analyzed by means of microbiological epidemiological markers. In 1990, Neisseria meningitidis serogroup B:4,7:P1.19,15, 1.7,1 sulfadiazine-resistant of the ET-5 complex emerged causing epidemic disease. Despite mass vaccination campaign (VaMengoc B+C®), the ET-5 clone remained hyperendemic after the epidemic peaked. In 1993 to 1995, an epidemic of serogroup C belonged to the cluster A4 overlapped, with a significant shift in the age distribution toward older age groups and an increase of sepsis. Serogroup C epidemics are a recurrent problem in Rio de Janeiro, which can be hindered with the introduction of a conjugate vaccine. We hope the data presented here brings useful information to discuss vaccines strategies and early management of suspected cases.


Subject(s)
Epidemics/statistics & numerical data , Meningitis, Meningococcal/epidemiology , Neisseria meningitidis/classification , Sepsis/epidemiology , Adolescent , Adult , Aged , Brazil/epidemiology , Child , Child, Preschool , Female , Humans , Incidence , Infant , Infant, Newborn , Male , Mass Vaccination , Meningitis, Meningococcal/microbiology , Middle Aged , Retrospective Studies , Sepsis/microbiology , Young Adult
2.
Braz. j. infect. dis ; 14(3): 242-251, May-June 2010. ilus, tab
Article in English | LILACS | ID: lil-556836

ABSTRACT

The main objectives of the present study were to investigate the clinical and laboratory features of meningococcal disease in the city of Rio de Janeiro, Brazil, during the overlap of 2 epidemics in the 1990s. We conducted a study of a series of cases of meningococcal disease admitted in a Meningitis Reference Hospital. All clinical isolates available were analyzed by means of microbiological epidemiological markers. In 1990, Neisseria meningitidis serogroup B:4,7:P1.19,15, 1.7,1 sulfadiazine-resistant of the ET-5 complex emerged causing epidemic disease. Despite mass vaccination campaign (VaMengoc B+C®), the ET-5 clone remained hyperendemic after the epidemic peaked. In 1993 to 1995, an epidemic of serogroup C belonged to the cluster A4 overlapped, with a significant shift in the age distribution toward older age groups and an increase of sepsis. Serogroup C epidemics are a recurrent problem in Rio de Janeiro, which can be hindered with the introduction of a conjugate vaccine. We hope the data presented here brings useful information to discuss vaccines strategies and early management of suspected cases.


Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Young Adult , Epidemics/statistics & numerical data , Meningitis, Meningococcal/epidemiology , Neisseria meningitidis/classification , Sepsis/epidemiology , Brazil/epidemiology , Incidence , Mass Vaccination , Meningitis, Meningococcal/microbiology , Retrospective Studies , Sepsis/microbiology , Young Adult
3.
Braz. j. med. biol. res ; 40(12): 1681-1687, Dec. 2007. graf, tab
Article in English | LILACS | ID: lil-466743

ABSTRACT

The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39, 40-59, and ³60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by biochemical analysis and toxigenicity was assessed by the Elek test. For diphtheria, full protection (antibodies ³0.1 IU/mL) was present in 84 percent of the individuals, 15 percent had basic protection (antibodies ³0.01 and <0.1 IU/mL) and 1 percent were susceptible (antibodies <0.01 IU/mL). Full tetanus protection (antibodies ³0.1 IU/mL) was present in 79 percent of the participants, 18 percent had basic protection (antibodies ³0.01 and <0.1 IU/mL) and 3 percent were susceptible (antibodies <0.01 IU/mL). The geometric mean of diphtheria and tetanus antibodies reached the highest values at 5-9 years and decreased until the 40-59-year age range, increasing again in individuals over 60 years. Three participants (0.8 percent) were carriers of C. diphtheriae, all non-toxigenic strains. The present results demonstrate the clear need of periodic booster for tetanus and diphtheria vaccine in adolescents and adults after primary immunization in childhood.


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Humans , Infant , Infant, Newborn , Middle Aged , Antibodies, Bacterial/blood , Clostridium tetani/immunology , Corynebacterium diphtheriae/immunology , Diphtheria/immunology , Tetanus/immunology , Age Distribution , Antibodies, Bacterial/immunology , Brazil , Diphtheria-Tetanus-Pertussis Vaccine/immunology , Diphtheria/prevention & control , Enzyme-Linked Immunosorbent Assay , Tetanus/prevention & control
4.
Braz J Med Biol Res ; 40(12): 1681-7, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17906776

ABSTRACT

The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39, 40-59, and > or =60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by biochemical analysis and toxigenicity was assessed by the Elek test. For diphtheria, full protection (antibodies > or =0.1 IU/mL) was present in 84% of the individuals, 15% had basic protection (antibodies > or =0.01 and <0.1 IU/mL) and 1% were susceptible (antibodies <0.01 IU/mL). Full tetanus protection (antibodies > or =0.1 IU/mL) was present in 79% of the participants, 18% had basic protection (antibodies > or =0.01 and <0.1 IU/mL) and 3% were susceptible (antibodies <0.01 IU/mL). The geometric mean of diphtheria and tetanus antibodies reached the highest values at 5-9 years and decreased until the 40-59-year age range, increasing again in individuals over 60 years. Three participants (0.8%) were carriers of C. diphtheriae, all non-toxigenic strains. The present results demonstrate the clear need of periodic booster for tetanus and diphtheria vaccine in adolescents and adults after primary immunization in childhood.


Subject(s)
Antibodies, Bacterial/blood , Clostridium tetani/immunology , Corynebacterium diphtheriae/immunology , Diphtheria/immunology , Tetanus/immunology , Adolescent , Adult , Age Distribution , Antibodies, Bacterial/immunology , Brazil , Child , Child, Preschool , Diphtheria/prevention & control , Diphtheria-Tetanus-Pertussis Vaccine/immunology , Enzyme-Linked Immunosorbent Assay , Humans , Infant , Infant, Newborn , Middle Aged , Tetanus/prevention & control
5.
Clin Microbiol Infect ; 12(1): 50-5, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16460546

ABSTRACT

Nasopharyngeal carriage of Streptococcus pneumoniae is a key factor in the development of invasive disease and the spread of resistant strains within the community. A single nasopharyngeal swab was obtained from 648 unvaccinated children aged <5 years, either healthy or with acute respiratory tract infection or meningitis, during the winters of 2000 and 2001. The overall pneumococcal carriage rate was 35.8% (95% CI 32.1-39.6). The pneumococcal serotypes found most frequently in the nasopharynx were 14, 6B, 6A, 19F, 10A, 23F and 18C, which included five of the seven serotypes in the currently licensed seven-valent conjugate vaccine (PCV7); serotypes 4 and 9V were less common. Serotypes 1 and 5 were isolated rarely from the nasopharynx. A comparison of 222 nasopharyngeal isolates with 125 invasive isolates, matched for age and time to the carrier isolates, showed a similar prevalence of penicillin non-susceptible pneumococci (PNSp) (19.8% and 19.2%, respectively). PNSp serotypes were similar (6B, 14, 19F, 19 A, 23B and 23F) for carriage and invasive disease isolates. The coverage of PCV7 for carriage isolates (52.2%) and invasive isolates (62.4%) did not differ significantly (p 0.06); similarly, there was no significant difference in PCV7 coverage for carriage isolates (34.5%) and invasive isolates (28.2%) of PNSp. These data suggest that PCV7 has the potential to reduce pneumococcal carriage and the number of carriers of PNSp belonging to vaccine serotypes.


Subject(s)
Streptococcus pneumoniae/classification , Anti-Bacterial Agents/pharmacology , Brazil/epidemiology , Carrier State/epidemiology , Carrier State/microbiology , Female , Humans , Infant , Male , Nasopharynx/microbiology , Penicillin Resistance , Penicillins/pharmacology , Pneumococcal Infections/epidemiology , Pneumococcal Infections/microbiology , Pneumococcal Vaccines/administration & dosage , Prevalence , Serotyping , Streptococcus pneumoniae/drug effects , Streptococcus pneumoniae/isolation & purification , Streptococcus pneumoniae/pathogenicity , Vaccination , Vaccines, Conjugate/administration & dosage
6.
Braz J Med Biol Res ; 35(11): 1293-300, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12426628

ABSTRACT

A total of 1712 strains of Haemophilus influenzae isolated from patients with invasive diseases were obtained from ten Brazilian states from 1996 to 2000. beta-Lactamase production was assessed and the minimum inhibitory concentrations (MIC) of ampicillin, chloramphenicol, ceftriaxone and rifampin were determined using a method for broth microdilution of Haemophilus test medium. The prevalence of strains producing beta-lactamase ranged from 6.6 to 57.7%, with an overall prevalence of 18.4%. High frequency of beta-lactamase-mediated ampicillin resistance was observed in Distrito Federal (25%), São Paulo (21.7%) and Paraná (18.5%). Of the 1712 strains analyzed, none was beta-lactamase negative, ampicillin resistant. A total of 16.8% of the strains were resistant to chloramphenicol, and 13.8% of these also presented resistance to ampicillin, and only 3.0% were resistant to chloramphenicol alone. All strains were susceptible to ceftriaxone and rifampin and the MIC90 were 0.015 micro g/ml and 0.25 micro g/ml, respectively. Ceftriaxone is the drug of choice for empirical treatment of bacterial meningitis in pediatric patients who have not been screened for drug susceptibility. The emergence of drug resistance is a serious challenge for the management of invasive H. influenzae disease, which emphasizes the fundamental role of laboratory-based surveillance for antimicrobial resistance.


Subject(s)
Anti-Bacterial Agents/pharmacology , Haemophilus influenzae/drug effects , Meningitis, Haemophilus/microbiology , beta-Lactamases/biosynthesis , Ampicillin Resistance , Brazil , Ceftriaxone/pharmacology , Child , Chloramphenicol Resistance , Drug Resistance, Bacterial , Haemophilus influenzae/enzymology , Haemophilus influenzae/isolation & purification , Humans , Microbial Sensitivity Tests , Rifampin/pharmacology
7.
Braz. j. med. biol. res ; 35(11): 1293-1300, Nov. 2002. tab
Article in English | LILACS | ID: lil-326253

ABSTRACT

A total of 1712 strains of Haemophilus influenzae isolated from patients with invasive diseases were obtained from ten Brazilian states from 1996 to 2000. ß-Lactamase production was assessed and the minimum inhibitory concentrations (MIC) of ampicillin, chloramphenicol, ceftriaxone and rifampin were determined using a method for broth microdilution of Haemophilus test medium. The prevalence of strains producing ß-lactamase ranged from 6.6 to 57.7 percent, with an overall prevalence of 18.4 percent. High frequency of ß-lactamase-mediated ampicillin resistance was observed in Distrito Federal (25 percent), Säo Paulo (21.7 percent) and Paraná (18.5 percent). Of the 1712 strains analyzed, none was ß-lactamase negative, ampicillin resistant. A total of 16.8 percent of the strains were resistant to chloramphenicol, and 13.8 percent of these also presented resistance to ampicillin, and only 3.0 percent were resistant to chloramphenicol alone. All strains were susceptible to ceftriaxone and rifampin and the MIC90 were 0.015 æg/ml and 0.25 æg/ml, respectively. Ceftriaxone is the drug of choice for empirical treatment of bacterial meningitis in pediatric patients who have not been screened for drug susceptibility. The emergence of drug resistance is a serious challenge for the management of invasive H. influenzae disease, which emphasizes the fundamental role of laboratory-based surveillance for antimicrobial resistance


Subject(s)
Humans , Child , Anti-Bacterial Agents , beta-Lactamases , Haemophilus influenzae , Meningitis, Haemophilus , Ampicillin Resistance , Brazil , Ceftriaxone , Chloramphenicol Resistance , Drug Resistance, Microbial , Haemophilus influenzae , Microbial Sensitivity Tests , Rifampin
8.
J Clin Microbiol ; 39(6): 2197-205, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11376057

ABSTRACT

Four hundred ninety-nine methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from 1996 to 1998 from 22 hospitals in five countries of Latin America-Argentina, Brazil, Chile, Uruguay and Mexico-were examined for antimicrobial susceptibility and clonal type in order to define the endemic clones in those hospitals. The hybridization of ClaI restriction digests with the mecA- and Tn554-specific DNA probes combined with pulsed-field gel electrophoresis of chromosomal SmaI digests (ClaI-mecA::ClaI-Tn554::PFGE clonal types) documented not only the predominance and persistence of the Brazilian clone (XI::B::B) in Brazil (97%) and Argentina (86%) but also its massive dissemination to Uruguay (100%). Moreover, a close relative of the Brazilian clone (XI::kappa::B) was highly represented in Chile (53%) together with a novel clone (47%) (II::E'::F) resistant to pencillin, oxacillin, ciprofloxacin, chloramphenicol, clindamycin, erythromycin, and gentamicin. A unique clonal type (I::NH::M) was detected in Mexico among pediatric isolates and was resistant to penicillin, oxacillin, and gentamicin only. This study clearly documented the very large capacity for geographic expansion and the persistence of the Brazilian clone, contributing not only to the increasing uniformity of the MRSA in South America but worldwide as well.


Subject(s)
Methicillin Resistance/genetics , Staphylococcal Infections/epidemiology , Staphylococcal Infections/microbiology , Staphylococcus aureus/classification , Staphylococcus aureus/drug effects , Anti-Bacterial Agents/pharmacology , DNA Transposable Elements/genetics , DNA, Bacterial/analysis , Deoxyribonucleases, Type II Site-Specific/metabolism , Electrophoresis, Gel, Pulsed-Field , Humans , Latin America/epidemiology , Microbial Sensitivity Tests , Nucleic Acid Hybridization , Staphylococcus aureus/genetics
9.
Braz J Med Biol Res ; 33(3): 295-300, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10719380

ABSTRACT

From 1989 to 1995, a total of 391 Haemophilus influenzae isolates were recovered from the cerebrospinal fluid (CSF) of hospitalized patients in São Paulo, Brazil. The majority of strains were isolated from infants aged less than 5 years. Strains belonging to biotype I (64.7%), biotype II (34.5%) and biotype IV (0.76%) were detected. Ninety-nine percent of these strains were serotype b. Minimal inhibitory concentration (MIC) was determined for ampicillin, chloramphenicol and ceftriaxone. The ss-lactamase assay was performed for all strains. The rate of ss-lactamase producer strains ranged from 10 to 21.4% during a period of 7 years, with an overall rate of 13.8%. Of the 391 strains analyzed, none was ss-lactamase negative ampicillin resistant (BLNAR). A total of 9.7% of strains showed resistance to both ampicillin and chloramphenicol; however, 4% of them were resistant to ampicillin only and 2% to chloramphenicol. All strains were susceptible to ceftriaxone and the MIC90 was 0.007 microg/ml, suggesting that ceftriaxone could be an option for the treatment of bacterial meningitis in pediatric patients who have not been screened for drug sensitivity.


Subject(s)
Anti-Bacterial Agents/pharmacology , Haemophilus influenzae/drug effects , Meningitis, Haemophilus/microbiology , Ampicillin/pharmacology , Ampicillin/therapeutic use , Anti-Bacterial Agents/therapeutic use , Brazil , Ceftriaxone/pharmacology , Ceftriaxone/therapeutic use , Child , Child, Preschool , Chloramphenicol/pharmacology , Chloramphenicol/therapeutic use , Drug Resistance, Microbial , Haemophilus influenzae/isolation & purification , Haemophilus influenzae/metabolism , Humans , Microbial Sensitivity Tests , beta-Lactamases/biosynthesis
10.
Braz. j. med. biol. res ; 33(3): 295-300, Mar. 2000. tab
Article in English | LILACS | ID: lil-255048

ABSTRACT

From 1989 to 1995, a total of 391 Haemophilus influenzae isolates were recovered from the cerebrospinal fluid (CSF) of hospitalized patients in São Paulo, Brazil. The majority of strains were isolated from infants aged less than 5 years. Strains belonging to biotype I (64.7 per cent), biotype II (34.5 per cent) and biotype IV (0.76 per cent) were detected. Ninety-nine percent of these strains were serotype b. Minimal inhibitory concentration (MIC) was determined for ampicillin, chloramphenicol and ceftriaxone. The ß-lactamase assay was performed for all strains. The rate of ß-lactamase producer strains ranged from 10 to 21.4 per cent during a period of 7 years, with an overall rate of 13.8 per cent. Of the 391 strains analyzed, none was ß-lactamase negative ampicillin resistant (BLNAR). A total of 9.7 per cent of strains showed resistance to both ampicillin and chloramphenicol; however, 4 per cent of them were resistant to ampicillin only and 2 per cent to chloramphenicol. All strains were susceptible to ceftriaxone and the MIC90 was 0.007 µg/ml, suggesting that ceftriaxone could be an option for the treatment of bacterial meningitis in pediatric patients who have not been screened for drug sensitivity.


Subject(s)
Humans , Child , Anti-Bacterial Agents/pharmacology , Haemophilus influenzae/drug effects , Meningitis, Haemophilus/drug therapy , Ampicillin/pharmacology , Ampicillin/therapeutic use , Anti-Bacterial Agents/therapeutic use , beta-Lactamases/biosynthesis , Brazil , Ceftriaxone/pharmacology , Ceftriaxone/therapeutic use , Chloramphenicol/pharmacology , Chloramphenicol/therapeutic use , Drug Resistance, Microbial , Haemophilus influenzae/isolation & purification , Haemophilus influenzae/metabolism , Microbial Sensitivity Tests
11.
Microb Drug Resist ; 4(3): 209-17, 1998.
Article in English | MEDLINE | ID: mdl-9818972

ABSTRACT

From January 1993 through December 1996, 1,252 Streptococcus pneumoniae strains from different geographic regions of Brazil were studied for penicillin (Pen) susceptibility. All pneumococci were isolated from normally sterile fluids from patients, newborns to 88 years old. Pen resistance (R) had a mean rate of 15.1%, with 14.5% of strains showing intermediate level Pen-R and 0.6% showing high-level Pen-R. Similar Pen-R rates were observed in different regions of the country, in the range of 9.5% to 17.1%. A Pen-R increase was noted from 9.6% in 1993 to 20.6% in 1996. Pen-R was mostly associated to serotypes 6B, 14, 19A, and 23F (89%). Chromosomal DNA relatedness of Pen-R strains was determined by pulsed field gel electrophoresis (PFGE). High genetic diversity was identified, being represented by 27 patterns among the 92 strains. Two important features were observed: the predominance of relatively low-level Pen MIC (range 0.1-0.5 mg/L) in 86 of the 92 strains, and the presence of 60.8% as four major PFGE clusters unique to Brazil. Another feature was the geographic spread of these clusters over large distances in the country. The city of São Paulo seems to be a Pen-R focus (18.4%) in Brazil. Only two strains representing the international clone B widely spread in France, Portugal, and Spain, belonging to serotype 14, were found.


Subject(s)
Penicillin Resistance/genetics , Streptococcus pneumoniae/drug effects , Brazil , Genetic Variation , Microbial Sensitivity Tests , Species Specificity , Streptococcus pneumoniae/classification , Streptococcus pneumoniae/genetics
12.
Microb Drug Resist ; 3(2): 141-6, 1997.
Article in English | MEDLINE | ID: mdl-9185141

ABSTRACT

A laboratory surveillance study was developed in Brazil in 1993 to determine capsular types and antimicrobial susceptibility of Streptococcus pneumoniae strains. By studying 360 strains isolated from children with invasive infections in three different cities, 8 out of 34 types were identified as being the most prevalent and considered as the reference group for further analyses. This group comprised 77.7% of all strains studied, and includes the types 1, 5, 6A/B, 9V, 14, 19F, 19A, and 23F. The prevalence of this reference group was significantly higher among strains isolated from children with pneumonia than meningitis. Similarly, this group was more prevalent among strains isolated from children 3 to 6 years of age than from children under 2 years of age. Most strains (78.6%) were found to be susceptible to penicillin and only 1.4% showed high resistance to this antibiotic. However, intermediate resistance to penicillin was detected in 20% of the strains. This laboratory surveillance will be maintained and extended to other cities of Brazil to better define and monitor the trends of pneumococcal infections for proper control and prevention.


Subject(s)
Drug Resistance, Microbial , Pneumococcal Infections/microbiology , Streptococcus pneumoniae/classification , Brazil/epidemiology , Child , Child, Preschool , Humans , Infant , Pneumococcal Infections/drug therapy , Pneumococcal Infections/epidemiology , Prevalence , Serotyping , Streptococcus pneumoniae/drug effects , Streptococcus pneumoniae/isolation & purification
13.
Rev Inst Med Trop Sao Paulo ; 37(4): 291-6, 1995.
Article in English | MEDLINE | ID: mdl-8599056

ABSTRACT

In the present study we report the results of an analysis, based on ribotyping of Corynebacterium diphtheriae intermedius strains isolated from a 9 years old child with clinical diphtheria and his 5 contacts. Quantitative analysis of RFLPs of rRNA was used to determine relatedness of these 7 C.diphtheriae strains providing support data in the diphtheria epidemiology. We have also tested those strains for toxigenicity in vitro by using the Elek's gel diffusion method and in vivo by using cell culture method on cultured monkey kidney cell (VERO cells). The hybridization results revealed that the 5 C.diphtheriae strains isolated from contacts and one isolated from the clinical case (nose case strain) had identical RFLP patterns with all 4 restriction endonucleases used, ribotype B. The genetic distance from this ribotype and ribotype A (throat case strain), that we initially assumed to be responsible for the illness of the patient, was of 0.450 showing poor genetic correlation among these two ribotypes. We found no significant differences concerned to the toxin production by using the cell culture method. In conclusion, the use of RFLPs of rRNA gene was successful in detecting minor differences in closely related toxigenic C.diphtheriae intermedius strains and providing information about genetic relationships among them.


Subject(s)
Corynebacterium diphtheriae/genetics , Diphtheria/microbiology , Adult , Bacterial Typing Techniques , Carrier State , Child , Child, Preschool , Corynebacterium diphtheriae/isolation & purification , Corynebacterium diphtheriae/physiology , Diphtheria/immunology , Diphtheria/transmission , Female , Humans , Infant , Male , Polymorphism, Restriction Fragment Length , RNA, Ribosomal
14.
Rev Inst Med Trop Sao Paulo ; 36(6): 531-7, 1994.
Article in English | MEDLINE | ID: mdl-7569628

ABSTRACT

A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. A total of 442 pleural fluid effusion samples (PFES) from children with clinical and laboratory diagnoses of acute bacterial pneumonia, plus 38 control PFES from tuberculosis patients and 20 negative control serum samples from healthy children were evaluated by Dot-ELISA. The samples were previously treated with 0.1M EDTA pH 7.5 at 90 degrees C for 10 min and dotted on nitrocellulose membrane. Pneumococcal omniserum diluted at 1:200 was employed in this assay for antigen detection. When compared with standard bacterial culture, counterimmunoelectrophoresis and latex agglutination techniques, the Dot-ELISA results showed relative indices of 0.940 to sensitivity, 0.830 to specificity and 0.760 to agreement. Pneumococcal omniserum proved to be an optimal polyvalent antiserum for the detection of pneumococcal antigen by Dot-ELISA. Dot-ELISA proved to be a practical alternative technique for the diagnosis of pneumococcal pneumonia.


Subject(s)
Antigens, Bacterial/analysis , Immunoblotting , Pleural Effusion/chemistry , Pneumonia, Pneumococcal/diagnosis , Polysaccharides, Bacterial/analysis , Streptococcus pneumoniae/immunology , Acute Disease , Child , Child, Preschool , Counterimmunoelectrophoresis , Humans , Infant , Infant, Newborn , Latex Fixation Tests , Pneumonia, Bacterial/diagnosis , Predictive Value of Tests , Sensitivity and Specificity
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