ABSTRACT
Detaching from work is beneficial because it helps employees recover from work demands. However, we argue that detachment may be a trade-off for employees in organizations with higher (vs. lower) levels of performance pressure. Drawing on social self-preservation theory, we hypothesize that evening detachment leads employees working in higher (vs. lower) performance pressure work contexts to experience increased shame at work the next morning. In turn, we hypothesize that shame motivates employees to engage in cheating behaviors to covertly inflate their performance and reduce the possibility that others will form negative perceptions of them. In three studies-a 2-week experience sampling study and two experiments-we find that evening detachment leads to heightened next-morning shame in higher (vs. lower) performance pressure work contexts, increasing cheating behavior throughout the workday. (PsycInfo Database Record (c) 2023 APA, all rights reserved).
Subject(s)
Deception , Workplace , Humans , ShameABSTRACT
Hepatosplenic gamma-delta (gammadelta) T-cell lymphoma is a rare but increasingly recognized lymphoid malignancy predominantly affecting young adult males. It is not well appreciated in the pediatric population. We report the third case of this aggressive lymphoma in a child as well as additional support for the consistency of the recently discovered cytogenetic abnormalities, isochromosome 7q and trisomy 8, which in this case were documented using fluorescence in situ hybridization (FISH) of a touch-preparation of the spleen.
Subject(s)
Chromosomes, Human, Pair 7/genetics , Chromosomes, Human, Pair 8/genetics , Isochromosomes/genetics , Liver Neoplasms/genetics , Lymphoma, T-Cell/genetics , Splenic Neoplasms/genetics , Trisomy/genetics , Child , Flow Cytometry , Humans , In Situ Hybridization, Fluorescence , Liver Neoplasms/pathology , Lymphoma, T-Cell/pathology , Male , Receptors, Antigen, T-Cell, gamma-delta/genetics , Splenic Neoplasms/pathologyABSTRACT
Genetic linkage studies have mapped the Huntington disease (HD) mutation to the distal region of the short arm of human chromosome 4. Analysis of recombination events in this region has produced contradictory locations for HD. One possible location is in the region distal to the D4S90 marker, which is located within 300 kilobases of the telomere. Other crossover events predict a more centromeric position for HD. Here we analyze the telomeric region of 4p in detail. Cloned DNA segments were derived from this region by utilizing a radiation-induced somatic cell hybrid as a source of DNA combined with preparative pulsed-field gel electrophoresis to enrich for the telomeric fraction. Additional DNA was obtained by using the cloned segments as multiple start points for cosmid walks. This strategy proved to be an effective method for cloning 250 kilobases of DNA in the region telomeric to D4S90. Hybridization analysis with the cloned DNA did not provide any evidence for the presence of rearrangements of 100 base pairs or greater in the DNA of individuals affected with HD. We also found no change in the size or structure of the 4p telomere in these samples.
Subject(s)
Chromosomes, Human, Pair 4 , DNA/genetics , Gene Rearrangement , Huntington Disease/genetics , Animals , Cell Line , Chromosome Mapping , Cloning, Molecular , DNA/isolation & purification , Genetic Linkage , Humans , Hybrid Cells/cytology , Mice , Nucleic Acid Hybridization , Restriction MappingABSTRACT
We have developed an X-irradiation:cell fusion procedure that segregates segments of human chromosomes lacking selectable markers and have used this approach to construct somatic cell hybrids retaining fragments of human chromosome 4 as the only human material. To identify hybrids retaining a small chromosomal fragment in the region of the Huntington disease (HD) gene, we used Southern blot analysis to screen 72 hybrid lines for the presence or absence of seven chromosome 4 single-copy loci. These data, combined with in situ hybridization experiments, identified three hybrids of interest. One of these cell lines, C25, stably retains a 10,000- to 20,000-kb fragment of distal 4p in the vicinity of the HD gene, translocated to a hamster chromosome. Field-inversion gel electrophoresis revealed no evidence of rearrangements in the human DNA present in C25. In combination with similar radiation hybrids, C25 is a valuable tool for isolating DNA probes near the HD gene.
Subject(s)
Chromosome Mapping/methods , Chromosomes, Human, Pair 4/ultrastructure , Huntington Disease/genetics , Animals , Blotting, Southern , Cell Fusion , Cricetinae , Cricetulus , Humans , Hybrid CellsABSTRACT
A radiation-induced hybrid cell line containing 10-20 million base pairs of DNA derived from the terminal part of human 4p16 in a background of hamster chromosomes has been used to construct a genomic library highly enriched for human sequences located close to the Huntington disease (HD) gene. Recombinant phage containing human inserts were isolated from this library and used as hybridization probes against two other radiation hybrids containing human fragments with chromosomal breaks in 4p16 and against a human-hamster somatic cell hybrid that retains only the 4p15-4pter part of chromosome 4. Of 121 human phage tested, 6 were mapped distal to the HD-linked D4S10 locus. Since the HD gene is located between D4S10 and the 4p telomere, all of these sequences are likely to be closer to HD than D4S10, and any one of them may be a distal flanking marker for the disease locus. Long-range restriction map analysis performed with a field-inversion gel system shows that the six new loci are distributed in different places within 4p16. Although it is not possible to establish an order for the six sequences with the FIGE data, the results demonstrate that the region detected by these probes must span at least 2000 kb of DNA.
