Do different standard plate counting (IDF/ISSO or AOAC) methods interfere in the conversion of individual bacteria counts to colony forming units in raw milk?

AIMS: This study aimed to establish the correlation between individual bacterial count (IBC) obtained by flow cytometry and the number of colony forming units (CFU) determined by standard plate count (SPC) in raw milk using two different reference methodologies: the methodology of the International Dairy Federation (IDF) - International Organization for Standardization (ISO) 4833, incubation for 72 h at 30°C and the methodology of the Association of Official Analytical Chemists (AOAC), incubation for 48 h at 35°C. METHODS AND RESULTS: For this, 100 bovine milk samples (80 ml) from different farms were collected in a sterile bottle and maintained refrigerated at 4°C and were delivered to the laboratory. In the laboratory, the samples were divided into two vials of 40 ml each. Then, half of the vials were forwarded for the SPC analysis, and the other half were analysed using the equipment BactoScan FC. The analyses by flow cytometry and SPC were performed at the same time (maximum deviation of +/- 1 h). To transform the data from IBC ml(-1) to CFU ml(-1) (IDF or AOAC methodology), a standard linear regression equation was used, as recommended by IDF/ISO-196. The difference between the reference methodologies affects the equation that transforms IBC into CFU and therefore the accuracy of the results. The results estimated by the equation using the ISO 4833 methodology were on average 0·18 log units higher than the results estimated using the equation using the AOAC methodology. CONCLUSIONS: After the comparison of the methodologies, it was concluded that there is an impact of the reference methodologies on the conversion of the results from IBC to CFU. SIGNIFICANCE AND IMPACT OF THE STUDY: Depending on the methodology adopted by each laboratory or country, there may not be equivalence in the results. Hence, the laboratories specialized in milk quality analysis that have changed their methodology for analysis, passing from the MAPA (AOAC) methodology to the IDF standard, need to develop new conversion equations to make their results in CFU ml(-1) consistent regardless of the reference methodology.

Genetic parameters for milk fatty acids, milk yield and quality traits of a Holstein cattle population reared under tropical conditions.

Information about genetic parameters is essential for selection decisions and genetic evaluation. These estimates are population specific; however, there are few studies with dairy cattle populations reared under tropical and sub-tropical conditions. Thus, the aim was to obtain estimates of heritability and genetic correlations for milk yield and quality traits using pedigree and genomic information from a Holstein population maintained in a tropical environment. Phenotypic records (n = 36 457) of 4203 cows as well as the genotypes for 57 368 single nucleotide polymorphisms from 755 of these cows were used. Covariance components were estimated using the restricted maximum likelihood method under a mixed animal model, considering a pedigree-based relationship matrix or a combined pedigree-genomic matrix. High heritabilities (around 0.30) were estimated for lactose and protein content in milk whereas moderate values (between 0.19 and 0.26) were obtained for percentages of fat, saturated fatty acids and palmitic acid in milk. Genetic correlations ranging from -0.38 to -0.13 were determined between milk yield and composition traits. The smaller estimates compared to other similar studies can be due to poor environmental conditions, which may reduce genetic variability. These results highlight the importance in using genetic parameters estimated in the population under evaluation for selection decisions.

Sazonalidade do teor de proteína e outros componentes do leite e sua relação com programa de pagamento por qualidade / Seasonability of protein and others milk components related with quality payment program

Avaliou-se o efeito da variação da qualidade do leite mediante acompanhamento de amostras provenientes de tanques refrigeradores. As 2.970 amostras foram obtidas de outubro/2005 a setembro/2006. Foram realizadas análises de composição, contagem de células somáticas (CCS) e contagem bacteriana total. As condições climáticas foram avaliadas pela temperatura e umidade do ar coletadas em estações meteorológicas distribuídas em todo o estado de São Paulo. No outono, ocorreram os maiores valores para gordura e proteína do leite, 3,65 e 3,21 por cento, respectivamente. Para contagem de células somáticas, foi observado aumento no verão e contagem bacteriana na época da primavera. Foi identificada a influência da época do ano sobre o preço do leite pago dentro de um programa, com valor de bonificação máxima de 7,9 por cento para o outono. Um dos mais importantes itens relacionados com a penalização foi a CCS, a qual pode interferir na qualidade dos produtos finais por meio de alteração da proporção de caseína/proteínas do soro. O efeito da CCS e o da época do ano devem ser considerados na formulação das tabelas de programas de pagamento.

This study evaluated the variation of quality of bulk tank milk using 2,970 samples, collected from October 2005 to September 2006. Milk composition analyses, somatic cell count (SCC), and total bacterial count were performed. Climate conditions were evaluated by temperature and relative air humidity collected at meteorological stations throughout São Paulo state. Autumn season was related to the highest values for protein and fat, 3.65 and 3.21 percent, respectively. For somatic cell count, it was observed an increase in the count in the summer and for bacterial count in the spring. The influence of season on milk price was observed for dairy producers in a program, with maximum bonus of 7.9 percent in the autumn. One of the most important parameters related to penalty for lower quality of milk was SCC, since it can affect the quality of some dairies by the alteration of casein/whey protein proportion. Therefore, the effects of SCC and season should be considered into the process of formulation of tables for milk payment program.

Short communication: evaluation of an on-farm test to estimate somatic cell count.

The objective of this study was to compare the results of an on-farm test, named Somaticell, with results of electronic cell counting and for milk somatic cell count (SCC) among readers. The Somaticell test correctly determined the SCC in fresh quarter milk samples. Correlation between Somaticell and electronic enumeration of somatic cells was 0.92 and kappa coefficient 0.82. Using a threshold of 205,000 cells/mL, the sensitivity and specificity for determination of intramammary infections were 91.3 and 96.0%, respectively. The SCC was greater for milk samples from which major mastitis pathogens were recovered. Minor variation among readers was observed and most likely associated with the mixing procedure. However, the final analysis indicated that this variation was not significant and did not affect the amount of samples classified as having subclinical mastitis. The on-farm test evaluated in this study showed adequate capacity of determining SCC on quarter milk samples and may be considered as an alternative for on-farm detection of subclinical mastitis.

Análise econômica de esquemas alternativos de controle leiteiro / Economic analysis of alternative schemes of milk recording

Compararam-se os custos de seis esquemas de controle leiteiro (CL), estabelecidos com base em diferentes freqüências de controles (com possibilidade de controle trimestral supervisionado e aproveitamento do CL da fazenda), no número de ordenhas e na realização de análises qualitativas (composição do leite e/ou contagem de células somáticas). Computaram-se os percentuais que os custos totais do CL representariam na receita bruta mensal do leite (CL/RB) e na receita líquida mensal (CL/RL). O esquema tradicional (freqüência mensal) apresentou o mais elevado custo mensal e os maiores CL/RB e CL/RL. Esquemas com intervalo maior entre controles e amostragem em ordenhas alternadas resultaram na redução dos custos com diárias e quilometragem. O custo mensal do CL variou de 0,68% a 1,8% sobre a receita bruta do leite e de 6,6% a 17,0% sobre a receita líquida.