ABSTRACT
We present a cohort of individuals who reached CD4+ T cell counts of greater than 1,000 cells/mm3 (Hypers) after starting antiretroviral treatment (ART) and compared them with those who reached between 350 and 999 CD4+ T cells/mm3 (Concordants). Demographic data, immune recovery kinetics, T CD4+ subset phenotypes, and integrated HIV DNA were analyzed. Data from individuals living with HIV on their first ART regimen and after 48 months of follow-up were obtained. Immune phenotype by Flow Cytometry analysis on whole blood was performed, cytokines were measured, and integrated HIV-1 DNA was measured by polymerase chain reaction. From a total of 424 individuals, 26 Hypers (6.1%), 314 Concordants (74.1%), and 84 (19.8%) discordants were identified. Hypers had a higher proportion of CD4+-naive (Nv) T cells (37.6 vs. 24.8, p < .05), and a low proportion of CD4+ effector memory T cells (27.9 vs. 39.4, p < .05), with similar results found in CD8+ T cells. Hypers demonstrated a higher percentage of CD4+CD45RA+CD31neg cells with a lower response to interleukin-2 stimulation and a lower integrated HIV-1 DNA/CD4 ratio (1.2 vs. 2.89, p < .05). In Hypers, T cell recovery occurs very early after initiation of ART. Following this initial recovery state, their CD4+ T cell level homeostasis seems to be driven by nonthymic-central-Nv cells. This exceptional recovery is associated with a lower HIV reservoir, which may be related to an increase in noninfected CD4+ T cells. These patients could then be eligible candidates for cure trials.
Subject(s)
CD8-Positive T-Lymphocytes , HIV Infections , Anti-Retroviral Agents/therapeutic use , CD4-Positive T-Lymphocytes , Cell Differentiation , HIV Infections/drug therapy , HumansABSTRACT
Background: Several studies have reported that variants rs16969968 G>A of the CHRNA5 gene and CYP2A6*12 of the CYP2A6 gene are associated with smoking and smoking refusal, respectively. In addition, some studies report that a higher cigarette consumption is associated with low body mass index (BMI). Aim: To analyze the allele and genotypic frequencies of these variants and their impact on smoking and BMI. Material and Methods: A blood sample was obtained and a survey about smoking habits was answered by 319 university students aged 18 to 35 years (127 women, 171 smokers), living in Northeastern Mexico. Genetic variants were studied by polymerase chain reaction/restriction fragment length polymorphism and their frequencies were associated with smoking and BMI. Results: No associations were found between the analyzed variants and smoking in the study groups. However, there was an association among non-smoking subjects between the A allele of rs16969968 and high a BMI (p < 0.01). Conclusions: This last variant may be involved in food-addiction disorders.
Subject(s)
Adolescent , Adult , Female , Humans , Male , Young Adult , Body Mass Index , /genetics , Gene Frequency , Nerve Tissue Proteins/genetics , Receptors, Nicotinic/genetics , Smoking/genetics , Cross-Sectional Studies , Genetic Variation/genetics , Genotype , Mexico , Nicotine/metabolism , Polymerase Chain Reaction/methods , Polymorphism, Genetic/geneticsABSTRACT
BACKGROUND: Several studies have reported that variants rs16969968 G>A of the CHRNA5 gene and CYP2A6*12 of the CYP2A6 gene are associated with smoking and smoking refusal, respectively. In addition, some studies report that a higher cigarette consumption is associated with low body mass index (BMI). AIM: To analyze the allele and genotypic frequencies of these variants and their impact on smoking and BMI. MATERIAL AND METHODS: A blood sample was obtained and a survey about smoking habits was answered by 319 university students aged 18 to 35 years (127 women, 171 smokers), living in Northeastern Mexico. Genetic variants were studied by polymerase chain reaction/restriction fragment length polymorphism and their frequencies were associated with smoking and BMI. RESULTS: No associations were found between the analyzed variants and smoking in the study groups. However, there was an association among non-smoking subjects between the A allele of rs16969968 and high a BMI (p < 0.01). CONCLUSIONS: This last variant may be involved in food-addiction disorders.
Subject(s)
Body Mass Index , Cytochrome P-450 CYP2A6/genetics , Gene Frequency , Nerve Tissue Proteins/genetics , Receptors, Nicotinic/genetics , Smoking/genetics , Adolescent , Adult , Cross-Sectional Studies , Female , Genetic Variation/genetics , Genotype , Humans , Male , Mexico , Nicotine/metabolism , Polymerase Chain Reaction/methods , Polymorphism, Genetic/genetics , Young AdultABSTRACT
The problems related to the means of transport in urban Brazilians have been getting worse over the years. The increase of the fleet, to the detriment of the adequacy and extent of the road network of cities contributes to this fact. This paper presents a profile of users of cycling in the city of Juiz de Fora, and thus contributes to the study of sustainable urban mobility, providing researchers with information that can guide the search for the encouragement of the use of means of transport to meet environmental needs urban centers that contributes to social equity.
Subject(s)
Accidents, Traffic/statistics & numerical data , Bicycling/statistics & numerical data , Environment Design , Transportation/methods , Urban Population , Adolescent , Adult , Aged , Aged, 80 and over , Brazil/epidemiology , Child , Female , Humans , Male , Middle Aged , Protective Devices/statistics & numerical data , Socioeconomic Factors , Young AdultABSTRACT
The historic cities of Brazil, despite its colonial structure, don't fail to go through transformations that affect contemporary cities, which is the main source of problems, leading to new approaches to urban issues such as mobility and accessibility. The uncontrolled growth of tourism in the historic cities can be considered as a big problem, because at the same time, they have committed to the conservation of its built heritage and demand control of the activities that occur in their areas without harm. Then, a permanent dialogue between conservatives and planners could be accomplished by joining the various sectoral policies. The study of urban mobility in historical sites was in fact the focus of this work because of their peculiarities, such as its specific characteristics of urban structure, morphology and occupation. In fact, the development of tourism in historic centers generates specific demands, such as adaptation to new uses of the houses, intensive movement of people and vehicles, illegal parking, among others. Beyond threatening the city preservation, does not provide mobility and accessibility to tourists, because these cities were not designed for the tourism conditions and needs of contemporary life. Characteristic features of Brazilian baroque cities, such as topography , the narrow streets, narrow or nonexistent sidewalks, steep turns and ramps strong, are not suitable for heavy vehicles, traffic and pedestrian circulation. Thus, studies concerning conservation urban integrated are aimed at an approach to interaction between historic preservation of the environment with the dynamic socio-economic of the local.
Subject(s)
Cities , Museums , Population Dynamics , Urbanization , HumansABSTRACT
Las ictiosis son un grupo de enfermedades con una elevada prevalencia en nuestro medio. Se caracterizan por la aparición de "escamas" en la piel. Dada su alta frecuencia de presentación, no es inhabitual que podamos atender a estos pacientes en nuestras consultas. Este artículo pretende profundizar en el conocimiento de este grupo de enfermedades y descubrirnos aspectos de las mismas que puedan ser de utilidad para el tratamiento de nuestros pacientes (AU)
Subject(s)
Male , Humans , Ichthyosis/diagnosis , Ichthyosis/classification , Genetic Diseases, Inborn/diagnosisABSTRACT
OBJECTIVE: This study sought to evaluate the prognostic significance of the presence of DNA of Chlamydia pneumoniae in the coronary atherosclerotic lesions of patients with unstable angina. BACKGROUND: C. pneumoniae has been implicated in the pathogenesis of coronary artery disease by serological and pathological studies, but whether antichlamydial antibodies and the presence of this pathogen in the coronary atherosclerotic tissue are related to prognosis in unstable angina remains unclear. METHODS: A total 76 coronary specimens from 45 patients with unstable angina undergoing bypass surgery were subjected to nested polymerase chain reaction (PCR) for C. pneumoniae. Antichlamydial immunoglobulin G (IgG), A (IgA) and M (IgM) were also examined by an enzyme immunoassay. Patients were followed during a 2-year period to determine the incidence of adverse cardiovascular events. RESULTS: DNA of C. pneumoniae was detected in 57 (75%) of 76 atherosclerotic lesions: 39 patients showed a positive PCR result in at least one plaque. Of the 45 patients, 44 (97.7%) showed a positive serological result: IgG was positive in 39 (86.6%) patients, IgM in five (11.1%) patients and IgA in 42 (93.3%). Clinical characteristics and serologic results were similarly distributed in patients with and without infected lesions at enrollment. At least one adverse event occurred in 21 (46.6%) of the 45 patients at 2 years: death in nine (20%), recurrent angina in 12 (26.6%), revascularization in six (13.3%) and myocardial infarction in two (4.4%) patients. The composite endpoint of death, myocardial infarction, recurrent angina and revascularization at 2-year follow-up did not differ according to the PCR or serologic results. CONCLUSIONS: The presence of C. pneumoniae in coronary atherosclerotic plaques of patients with unstable angina undergoing coronary bypass grafting does not have prognostic significance. In addition, serology does not allow us to differentiate those patients with plaque infection by C. pneumoniae and also does not provide any prognostic information in these patients.
Subject(s)
Angina, Unstable/microbiology , Chlamydia Infections/epidemiology , Chlamydophila pneumoniae/isolation & purification , Coronary Artery Bypass , Coronary Artery Disease/microbiology , Coronary Vessels/microbiology , Aged , Angina, Unstable/complications , Angina, Unstable/surgery , Chi-Square Distribution , Chlamydia Infections/complications , Coronary Artery Disease/complications , Coronary Artery Disease/surgery , Female , Humans , Male , Middle Aged , Polymerase Chain Reaction , Prognosis , Prospective Studies , Serologic TestsABSTRACT
BACKGROUND: Chlamydia pneumoniae has been associated with coronary artery disease by both seroepidemiological studies, and by direct detection of the micro-organism in atherosclerotic lesions. This bacteria could play a potential role in the development of acute coronary events. We examined coronary arteries from patients with unstable angina in order to verify an endovascular presence of C. pneumoniae, and to determine if there is any relationship between serology of acute infection by this pathogen and its presence inside the atherosclerotic plaque of these patients. METHODS: We analysed a total of 76 atherosclerotic plaques obtained from 45 patients who underwent coronary artery bypass surgery. In all patients unstable angina was present within the prior 3 weeks. The presence of C. pneumoniae in the plaque was determined by nested polymerase chain reaction (PCR). Antichlamydial immunoglobulin G (IgG), A (IgA) and M (IgM) was examined by microimmunofluorescence and compared to the PCR result. FINDINGS: DNA of C. pneumoniae was detected in 57 (75%) of 76 atherosclerotic lesions. In most cases (74/76: 97%) a positive IgA, IgM or IgG result was seen. Seven (12%) and 54 (94%) of the 57 PCR positive plaques came from patients with a positive IgM and IgA result, respectively. There was no statistical significant difference between PCR positive and PCR negative plaques in patients with a positive or negative serological result. Clinical characteristics were similarly distributed in patients with and without infected lesions. INTERPRETATION: C. pneumoniae organisms are frequently found in the atherosclerotic lesions of patients undergoing coronary surgery for unstable angina. Neither serological results of acute or recent infection by C. pneumoniae nor clinical characteristics are useful in predicting the individual risk of harbouring C. pneumoniae in the coronary lesions of patients with unstable angina.
Subject(s)
Angina, Unstable/immunology , Angina, Unstable/microbiology , Chlamydia Infections/epidemiology , Chlamydophila pneumoniae/isolation & purification , Coronary Artery Disease/microbiology , Coronary Artery Disease/pathology , Coronary Vessels/microbiology , Adult , Aged , Angina, Unstable/pathology , Chlamydia Infections/complications , Chlamydia Infections/immunology , Chlamydia Infections/pathology , Coronary Artery Bypass , Coronary Artery Disease/complications , Coronary Vessels/pathology , Female , Humans , Immunoglobulins/blood , Male , Middle Aged , Polymerase Chain Reaction/methods , Prevalence , Prospective Studies , Seroepidemiologic Studies , Serologic TestsABSTRACT
La feminización testicular es un síndrome en el cual se encuentra insensibilidad a los andrógenos, el cual puede presentarse en forma completa o incompleta. Se presenta el caso de una joven de 17 años de edad con el Síndrome de insensibilidad completa a los andrógenos. La paciente acudió a consulta médica por presentar amenorrea primaria como única patología, con genitales externos femeninos normales. El diagnóstico, hallazgos, manejo y seguimiento en cinco meses son descritos, así como una revisión del tema
Subject(s)
Humans , Female , Adult , Androgens/deficiency , Androgen-Insensitivity Syndrome/complicationsABSTRACT
El diagnóstico prenatal de gemelos unidos bicéfalos por ultrasonografía, una revisión de esta patología y las implicaciones de su manejo obstétrico son descritas en este caso clínico
Subject(s)
Humans , Male , Female , Infant, Newborn , Adult , Congenital Abnormalities/genetics , Prenatal Diagnosis , Twins, Conjoined/physiopathologyABSTRACT
Del 1§ de noviembre de 1987 al 31 de enero de 1988 se estudiaron 60 pacientes con embarzo de término y diagnóstico obvio de ruptura prematura de membranas (RPM). De manera aleatoria a un grupo se les administró antibiótico como profilaxis y al otro no. Del total sólo se presentaron dos casos de morbilidad infecciosa, uno en cada grupo. Se concluye que en embarazos de término, complicados por RPM sin otros factores de riesgos agregados, los antibióticos administrados como profilaxis, no modifican la frecuencia de morbilidad infecciosa puerperal
