Genetics and physiology of Salmonella houtenae isolated from a river in Mexico provides insight into the aquatic habitat influence on its adaptation and pathogenesis.

Salmonella enterica subsp. houtenae (IV) is a non-enteric subspecies of the genus Salmonella and has recently been implicated in extraintestinal diseases in humans. In Mexico, its reported that rivers are a reservoir of Salmonella houtenae, however, detailed information about the virulence and infective capacity of this bacterium are limited. Here, we present the high-quality genome draft of Salmonella houtenae str. CFSAN039533 isolated from a river in Culiacan, Mexico. In addition, its virulence, antimicrobial resistance profile and the use of carbon sources for its primary metabolism are analyzed. The genome sequence of CFSAN03953 strain comprises 4.74-Mb which contains 4266 protein coding genes and 77 tRNA genes. The strain belongs to the Marine serotype (48: g, z51: -), and showed several virulence genes related to the SPI-1, SPI-2, SPI-3, SPI-5 and fimbriae genes. The strain exhibited a susceptible phenotype, which contrasted with the resistance genes of aminoglycosides family identified into the genome. Carbon source degradation evaluation along with the genomic analysis, indicated that the strain can use a relatively broad spectrum of compounds related to the central metabolic pathways. This study provides information on the genetics and physiology of Salmonella houtenae that confirms its ability to survive and adapt to the environment. In addition, its pathogenic potential and infective capacity towards warm-blooded hosts are demonstrated.

Pathogenic potential of non-typhoidal Salmonella serovars isolated from aquatic environments in Mexico.

BACKGROUND: River water has been implicated as a source of non-typhoidal Salmonella (NTS) serovars in Mexico. OBJECTIVE: To dissect the molecular pathogenesis and defense strategies of seven NTS strains isolated from river water in Mexico. METHODS: The genome of Salmonella serovars Give, Pomona, Kedougou, Stanley, Oranienburg, Sandiego, and Muenchen were sequenced using the whole-genome shotgun methodology in the Illumina Miseq platform. The genoma annotation and evolutionary analyses were conducted in the RAST and FigTree servers, respectively. The MLST was performed using the SRST2 tool and the comparisons between strains were clustered and visualized using the Gview server. Experimental virulence assay was included to evaluate the pathogenic potential of strains. RESULTS: We report seven high-quality draft genomes, ranging from ~ 4.61 to ~ 5.12 Mb, with a median G + C value, coding DNA sequence, and protein values of 52.1%, 4697 bp, and 4,589 bp, respectively. The NTS serovars presented with an open pan-genome, offering novel genetic content. Each NTS serovar had an indistinguishable virulotype with a core genome (352 virulence genes) closely associated with Salmonella pathogenicity; 13 genes were characterized as serotype specific, which could explain differences in pathogenicity. All strains maintained highly conserved genetic content regarding the Salmonella pathogenicity islands (1-5) (86.9-100%), fimbriae (84.6%), and hypermutation (100%) genes. Adherence and invasion capacity were confirmed among NTS strains in Caco-2 cells. CONCLUSION: Our results demonstrated the arsenal of virulence and defense molecular factors harbored on NTS serovars and highlight that environmental NTS strains are waterborne pathogens worthy of attention.