ABSTRACT
Autologous platelet concentrates have been used in regenerative medicine in humans due to the abundance of growth factors, but there are only a few reports in small animals. This study aimed to prepare and characterize a leukocyte and platelet-rich fibrin membrane (L-PRF) produced with blood obtained from cats. Thirteen client-owned healthy adult Maine Coon cats were enrolled. The blood samples were collected and centrifuged at 650g for 12 min using a centrifuge specifically designed for this application. The L-PRF clot was removed from the tube and red blood cell base layer was separated, leaving buffy coat intact. After this, L-PRF clot was compressed by specialized metal plate for 30-60 s, and L-PRF membrane was obtained. Light microscopy examination of the membranes showed three distinct layers: white part, buffy coat, and red part. Immunohistochemical analysis demonstrated expression of vascular endothelial growth factor and platelet derived growth factor. The scanning electron microscopy showed that three-dimensional architecture of fibrin network was more compact in the area near the buffy coat. In conclusion, the method used allowed the characterization of the L-PRF membrane composition, which presented cell types and fibrin network architecture similar to those described in the human species.
Subject(s)
Platelet-Rich Fibrin , Animals , Blood Platelets , Cats , Fibrin , Humans , Leukocytes , Vascular Endothelial Growth Factor AABSTRACT
This study aimed to assess joint angle values in the forelimbs and hind limbs of crab-eating foxes ( Cerdocyon thous) using a conventional manual goniometer. Nine captive crab-eating foxes, aged over 1 yr old, weighing from 5.9 to 7.5 kg were used. The maximum flexion and maximum extension of the shoulder, elbow, carpal, hip, stifle, and tarsal joints were measured. The range of motion was determined through the difference between the maximum flexion and the maximum extension. There was no significant difference between the right and left sides in the goniometric measurements. The mean values of the range of motion for shoulder, elbow, carpal, hip, stifle, and tarsal joints were respectively 108°, 99°, 131°, 79°, 103°, and 86°. The goniometric values of the crab-eating foxes can be used as means of comparison with other animals from the same species for diagnostic purposes, research documentation, and evaluation of therapeutic procedures.
Subject(s)
Animals, Zoo/physiology , Canidae/physiology , Forelimb/physiology , Hindlimb/physiology , Range of Motion, Articular , Animals , Arthrometry, Articular/veterinary , Brazil , Female , Forelimb/diagnostic imaging , Hindlimb/diagnostic imaging , Male , RadiographyABSTRACT
Six cases of mandibular fractures associated with severe periodontal disease that had been treated by mandibulectomy, due to intense bone loss, were evaluated retrospectively. The dogs were mainly older, small breed dogs that had suffered a traumatic event. Four dogs had a bilateral mandibulectomy and 2 a unilateral mandibulectomy.
Mandibulectomie pour le traitement de fractures associées à une maladie parodontale grave. Six cas de fractures mandibulaires associées à une maladie parodontale grave qui avaient été traités à l'aide d'une mandibulectomie, en raison d'une perte osseuse intense, ont été évalués rétrospectivement. Les chiens étaient pour la plupart âgés et de petite race et ils avaient été victimes d'un traumatisme. Quatre chiens avaient subi une madibulectomie bilatérale et 2 une mandibulectomie unilatérale.(Traduit par Isabelle Vallières).
