The pace of mitochondrial molecular evolution varies with seasonal migration distance.

Animals that engage in long-distance seasonal migration experience strong selective pressures on their metabolic performance and life history, with potential consequences for molecular evolution. Species with slow life histories typically show lower rates of synonymous substitution (dS) than "fast" species. Previous research suggests long-distance seasonal migrants have a slower life history strategy than short-distance migrants, raising the possibility that rates of molecular evolution may covary with migration distance. Additionally, long-distance migrants may face strong selection on metabolically-important mitochondrial genes due to their long-distance flights. Using over 1,000 mitochondrial genomes, we assessed the relationship between migration distance and mitochondrial molecular evolution in 39 boreal-breeding migratory bird species. We show that migration distance correlates negatively with dS, suggesting that the slow life history associated with long-distance migration is reflected in rates of molecular evolution. Mitochondrial genes in every study species exhibited evidence of purifying selection, but the strength of selection was greater in short-distance migrants, contrary to our predictions. This result may indicate effects of selection for cold tolerance on mitochondrial evolution among species overwintering at high latitudes. Our study demonstrates that the pervasive correlation between life history and molecular evolutionary rates exists in the context of differential adaptations to seasonality.

Space weather disrupts nocturnal bird migration.

Space weather, including solar storms, can impact Earth by disturbing the geomagnetic field. Despite the known dependence of birds and other animals on geomagnetic cues for successful seasonal migrations, the potential effects of space weather on organisms that use Earth's magnetic field for navigation have received little study. We tested whether space weather geomagnetic disturbances are associated with disruptions to bird migration at a macroecological scale. We leveraged long-term radar data to characterize the nightly migration dynamics of the nocturnally migrating North American avifauna over 22 y. We then used concurrent magnetometer data to develop a local magnetic disturbance index associated with each radar station (ΔBmax), facilitating spatiotemporally explicit analyses of the relationship between migration and geomagnetic disturbance. After controlling for effects of atmospheric weather and spatiotemporal patterns, we found a 9 to 17% decrease in migration intensity in both spring and fall during severe space weather events. During fall migration, we also found evidence for decreases in effort flying against the wind, which may represent a depression of active navigation such that birds drift more with the wind during geomagnetic disturbances. Effort flying against the wind in the fall was most reduced under both overcast conditions and high geomagnetic disturbance, suggesting that a combination of obscured celestial cues and magnetic disturbance may disrupt navigation. Collectively, our results provide evidence for community-wide avifaunal responses to geomagnetic disturbances driven by space weather during nocturnal migration.

Rapid adjustments of migration and life history in hemisphere-switching cliff swallows.

Many aspects of bird migration are necessarily innate.1 However, the extent of deterministic genetic control, environmental influence, and individual decision making in the control of migration remains unclear.2-8 Globally, few cases of rapid and dramatic life-history changes resulting in novel migration strategies are known. An example is latitudinal trans-hemispheric breeding colonization, whereby a subpopulation suddenly begins breeding on its non-breeding range.9-13 These life-history reversals demand concomitant changes in the timing of migration, feather molt, and breeding if the population is to remain viable.13 Cliff swallows, Petrochelidon pyrrhonota, are long-distance migrants that breed in North America and spend the non-breeding season mostly in South America.14 However, in 2015, a small population switched hemispheres by breeding successfully in Argentina,9 over 8,000 km from the nearest potential source, after presumably failed attempts.15,16 This provided a unique chance to characterize the early mechanisms of change in migratory behavior and phenology and to assess the possibility of double breeding. We tracked cliff swallows with geolocators following their second and fourth breeding seasons in Argentina, documenting inverted seasonality, three new migratory patterns and non-breeding areas (North America, Mesoamerica, and South America), and a shift of molt phenology by approximately 6 months, all possibly arising within a single generation. These birds did not practice migratory double breeding, although some spent the boreal summer in the traditional breeding range. Our data show that fundamental phenological changes occurred very rapidly during colonization and that phenotypic plasticity can underlie profound changes in the life histories of migratory birds.

Quantification of anatomical variation at the atlanto-occipital articulation: morphometric resolution of commingled human remains within the repatriation documentation process.

Within many institutional collections are skeletal and mummified human remains representing a part of our species' adaptation and evolution to various biocultural environments. Archaeologically recovered individuals come from deep into our past, and possess information that provides insight into population history, genetics, diet, health and other questions relevant to all living peoples. Academic concerns have been raised regarding the reinterment of these collections due to the rise of the international repatriation movement, the passage of various laws and implementation of institutional policies. While all potential research questions cannot be anticipated, the proactive documentation of collections is one way to ensure primary data are maintained for future study. This paper explores developments in digitization technology that allow the archive of virtual copies of human remains, and an example of how anatomical and archaeological collections can be digitized towards pragmatic research goals. The anatomical variability of the human atlanto-occipital (AO) articular surfaces was studied using non-metric categorical shape, 2D measurement and 3D morphometric analyses to provide reference standards for the reassociation of individuals from commingled skeletal remains, such as found in some archaeological sites or forensic investigations including mass grave or mass disaster recovery scenes. Results suggest that qualitative shape observations and caliper-derived measurements of the articulating AO condyles tend to display significant sexual dimorphism and biological ancestry-related size and shape differences. Variables derived from a scanned 3D mesh, such as condylar angle and articular surface curvature, quantify biomechanical variation and display a stronger congruency within individuals. It is recommended that a two-stage approach involving initial screening and identification of possible reassociation candidates is accomplished with a linear osteometric approach, followed by 3D laser scanning of the candidate joint surfaces for morphometric analyses to confirm reassociations when destructive DNA typing is not allowed or otherwise impractical due to cost or other resource restrictions.

Solar-powered radio tags reveal patterns of post-fledging site visitation in adult and juvenile Tree Swallows Tachycineta bicolor.

The availability of small, lightweight tracking devices enhances our ability to study birds during mobile phases of their lives. Tree Swallows Tachycineta bicolor, a model species of wild songbird, are well-studied during their breeding season; but our understanding of their biology at other times of the year, when they are not tied to the fixed location of a nest, is more limited. We developed a lightweight radio tag with no battery (solar nanotag) to study the movements of small animals, and we deployed it to explore the behavior of Tree Swallows after the end of their summer breeding season. We tagged 32 breeding adult swallows and 36 juveniles and monitored their presence and absence at the breeding site during the post-fledging period. Although our observations are based on very small sample sizes, the tags revealed previously unknown patterns in Tree Swallow behavior during the post-breeding season. Some Tree Swallow fledglings continued to visit the site repeatedly in the months following the nesting season, with the latest detection occurring on September 30th; by contrast, all adults had permanently departed by the end of July. These results inform future hypotheses about post-breeding movements in Tree Swallows. But, more generally, the detection of tagged swallows on their distant wintering grounds, seven months after tagging, indicates the potential of studying small passerine movements throughout their entire lifetimes, and suggests a rich array of applications for these "Life Tags" to study the movements of small animals world-wide.

Shock attenuation in the human lumbar spine during walking and running.

During locomotion, each step generates a shock wave that travels through the body toward the head. Without mechanisms for attenuation, repeated shocks can lead to pathology. Shock attenuation (SA) in the lower limb has been well studied, but little is known about how posture affects SA in the spine. To test the hypothesis that lumbar lordosis (LL) contributes to SA, 27 adults (14 male, 13 female) walked and ran on a treadmill. Two lightweight, tri-axial accelerometers were affixed to the skin overlying T12/L1 and L5/S1. Sagittal plane accelerations were analyzed using power spectral density analysis, and lumbar SA was assessed within the impact-related frequency range. 3D kinematics quantified dynamic and resting LL. To examine the effects of intervertebral discs on spinal SA, supine MRI scans were used to measure disc morphology. The results showed no association between LL and SA during walking, but LL correlated with SA during running (P<0.01, R2=0.30), resulting in as much as 64% reduction in shock signal power among individuals with the highest LL. Patterns of lumbar spinal motion partially explain differences in SA: larger amplitudes of LL angular displacement and slower angular displacement velocity during running were associated with greater lumbar SA (P=0.008, R2=0.41). Intervertebral discs were associated with greater SA during running (P=0.02, R2=0.22) but, after controlling for disc thickness, LL remained strongly associated with SA (P=0.001, R2=0.44). These findings support the hypothesis that LL plays an important role in attenuating impact shocks transmitted through the human spine during high-impact, dynamic activities such as running.

Interacting effects of land use and climate on rodent-borne pathogens in central Kenya.

Understanding the effects of anthropogenic disturbance on zoonotic disease risk is both a critical conservation objective and a public health priority. Here, we evaluate the effects of multiple forms of anthropogenic disturbance across a precipitation gradient on the abundance of pathogen-infected small mammal hosts in a multi-host, multi-pathogen system in central Kenya. Our results suggest that conversion to cropland and wildlife loss alone drive systematic increases in rodent-borne pathogen prevalence, but that pastoral conversion has no such systematic effects. The effects are most likely explained both by changes in total small mammal abundance, and by changes in relative abundance of a few high-competence species, although changes in vector assemblages may also be involved. Several pathogens responded to interactions between disturbance type and climatic conditions, suggesting the potential for synergistic effects of anthropogenic disturbance and climate change on the distribution of disease risk. Overall, these results indicate that conservation can be an effective tool for reducing abundance of rodent-borne pathogens in some contexts (e.g. wildlife loss alone); however, given the strong variation in effects across disturbance types, pathogen taxa and environmental conditions, the use of conservation as public health interventions will need to be carefully tailored to specific pathogens and human contexts.This article is part of the themed issue 'Conservation, biodiversity and infectious disease: scientific evidence and policy implications'.

Testing biomechanical models of human lumbar lordosis variability.

OBJECTIVES: Lumbar lordosis (LL) is a key adaptation for bipedalism, but factors underlying curvature variations remain unclear. This study tests three biomechanical models to explain LL variability. MATERIALS AND METHODS: Thirty adults (15 male, 15 female) were scanned using magnetic resonance imaging (MRI), a standing posture analysis was conducted, and lumbar range of motion (ROM) was assessed. Three measures of LL were compared. The trunk's center of mass was estimated from external markers to calculate hip moments (Mhip ) and lumbar flexion moments. Cross-sectional areas of lumbar vertebral bodies and trunk muscles were measured from scans. Regression models tested associations between LL and the Mhip moment arm, a beam bending model, and an interaction between relative trunk strength (RTS) and ROM. RESULTS: Hip moments were not associated with LL. Beam bending was moderately predictive of standing but not supine LL (R2 = 0.25). Stronger backs and increased ROM were associated with greater LL, especially when standing (R2 = 0.65). The strength-flexibility model demonstrates the differential influence of RTS depending on ROM: individuals with high ROM exhibited the most LL variation with RTS, while those with low ROM showed reduced LL regardless of RTS. DISCUSSION: Hip moments appear constrained suggesting the possibility of selection, and the beam model explains some LL variability due to variations in trunk geometry. The strength-flexibility interaction best predicted LL, suggesting a tradeoff in which ROM limits the effects of back strength on LL. The strength-flexibility model may have clinical relevance for spinal alignment and pathology. This model may also suggest that straight-backed Neanderthals had reduced lumbar mobility.

Physical fitness differences between rural and urban children from western Kenya.

OBJECTIVES: To study the effects of urbanization on physical fitness (PF), we compare PF between urban and rural children from western Kenya. We hypothesize that active rural children are stronger, more flexible, and have greater endurance, and that PF differences are predictive of endurance running performance. METHODS: We recruited an age-matched, cross-sectional sample of participants (55 males, 60 females; 6-17 years) from schools near Eldoret, Kenya. PF and anthropometrics were assessed using the FITNESSGRAM®. General linear mixed models (GLMM) and path analyses tested for age, sex, and activity group differences in PF, as well as the effects of PF variables on mile run time. RESULTS: On average, urban participants had greater body mass (36.8 ± 15.9 vs. 31.9 ± 10.9 kg) but were not taller than rural participants (1.4 ± 0.2 vs. 1.4 ± 0.2 cm). Greater urban body mass appears driven by higher body fat (28.2 ± 9.4 vs. 16.8 ± 4.4%), which increased with age in urban but not rural participants. GLMM analyses showed age effects on strength variables (P<0.05) and sex differences in hip flexibility, sit-ups, and mile run (P<0.05). There were few differences in PF between groups except rural participants had stronger back muscles (18.2 ± 4.5 vs. 14.18 ± 4.3 cm) and faster mile times (6.3 ± 0.7 vs. 7.9 ± 2.0 min). Body composition and abdominal strength were predictive of mile time (P < 0.06), but the path analysis revealed a network of interacting direct and indirect effects that influenced endurance performance. CONCLUSIONS: Although differences in endurance and body composition are marked between urban and rural groups, strength and flexibility are not always correlated with overall activity levels. Am. J. Hum. Biol. 28:514-523, 2016. © 2015 Wiley Periodicals, Inc.

Effects of stride frequency and foot position at landing on braking force, hip torque, impact peak force and the metabolic cost of running in humans.

Endurance runners are often advised to use 90 strides min(-1), but how optimal is this stride frequency and why? Endurance runners are also often advised to maintain short strides and avoid landing with the feet too far in front of their hips or knees (colloquially termed 'overstriding'), but how do different kinematic strategies for varying stride length at the same stride frequency affect economy and impact peaks? Linear mixed models were used to analyze repeated measures of stride frequency, the anteroposterior position of the foot at landing, V̇O2 , lower extremity kinematics and vertical ground reaction forces in 14 runners who varied substantially in height and body mass and who were asked to run at 75, 80, 85, 90 and 95 strides min(-1) at 3.0 m s(-1). For every increase of 5 strides min(-1), maximum hip flexor moments in the sagittal plane increased by 5.8% (P<0.0001), and the position of the foot at landing relative to the hip decreased by 5.9% (P=0.003). Higher magnitudes of posteriorly directed braking forces were associated with increases in foot landing position relative to the hip (P=0.0005) but not the knee (P=0.54); increases in foot landing position relative to the knee were associated with higher magnitudes (P<0.0001) and rates of loading (P=0.07) of the vertical ground reaction force impact peak. Finally, the mean metabolically optimal stride frequency was 84.8±3.6 strides min(-1), with 50.4% of the variance explained by the trade-off between minimizing braking forces versus maximum hip flexor moments during swing. The results suggest that runners may benefit from a stride frequency of approximately 85 strides min(-1) and by landing at the end of swing phase with a relatively vertical tibia.

Variation in Foot Strike Patterns among Habitually Barefoot and Shod Runners in Kenya.

Runners are often categorized as forefoot, midfoot or rearfoot strikers, but how much and why do individuals vary in foot strike patterns when running on level terrain? This study used general linear mixed-effects models to explore both intra- and inter-individual variations in foot strike pattern among 48 Kalenjin-speaking participants from Kenya who varied in age, sex, body mass, height, running history, and habitual use of footwear. High speed video was used to measure lower extremity kinematics at ground contact in the sagittal plane while participants ran down 13 meter-long tracks with three variables independently controlled: speed, track stiffness, and step frequency. 72% of the habitually barefoot and 32% of the habitually shod participants used multiple strike types, with significantly higher levels of foot strike variation among individuals who ran less frequently and who used lower step frequencies. There was no effect of sex, age, height or weight on foot strike angle, but individuals were more likely to midfoot or forefoot strike when they ran on a stiff surface, had a high preferred stride frequency, were habitually barefoot, and had more experience running. It is hypothesized that strike type variation during running, including a more frequent use of forefoot and midfoot strikes, used to be greater before the introduction of cushioned shoes and paved surfaces.

Effects of pole compliance and step frequency on the biomechanics and economy of pole carrying during human walking.

This study investigates whether a flexible pole can be used as an energy-saving method for humans carrying loads. We model the carrier and pole system as a driven damped harmonic oscillator and predict that the energy expended by the carrier is affected by the compliance of the pole and the ratio between the pole's natural frequency and the carrier's step frequency. We tested the model by measuring oxygen consumption in 16 previously untrained male participants walking on a treadmill at four step frequencies using two loaded poles: one made of bamboo and one of steel. We found that when the bamboo pole was carried at a step frequency 20% greater than its natural frequency, the motions of the centers of mass of the load and carrier were approximately equal in amplitude and opposite in phase, which we predicted would save energy for the carrier. Carrying the steel pole, however, resulted in the carrier and loads oscillating in phase and with roughly equal amplitude. Although participants were less economical using poles than predicted costs using conventional fixed-load techniques (such as backpacks), the bamboo pole was on average 5.0% less costly than the steel pole. When allowed to select their cadence, participants also preferred to carry the bamboo pole at step frequencies of ∼2.0 Hz. This frequency, which is significantly higher than the preferred unloaded step frequency, is most economical. These experiments suggest that pole carriers can intuitively adjust their gaits, or choose poles with appropriate compliance, to increase energetic savings.