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J Biotechnol ; 389: 78-85, 2024 Jun 20.
Article in English | MEDLINE | ID: mdl-38718873

ABSTRACT

In a bid to explore the on-site biorefinery approach for conversion of forestry residues, lignocellulosic biomass into value-added products was studied. The bark white pine wood was subjected to the microwave technique of fast and slow hydrolysis under varying acid and biomass concentrations to produce levulinic acid (LA). The HCl (2% v/v) and plant biomass (1% w/v) were identified as the optimum conditions for fast wood hydrolysis (270 ºC for 12 sec), which led to maximum LA yield of 446.68 g/kgPB. The proposed sustainable approach is mild, quick, and utilized a very low concentration of the HCl for the production of LA. The hydrolysate was used as a medium for Kluyveromyces marxianus growth to produce 2-phenylethanol (2-PE). K. marxianus used 74-95% of furfural from hydrolysate as a co-substrate to grow. The proposed model of the integrated biorefinery is an affordable on-site approach of using forest waste into localized solutions to produce LA and 2-PE.


Subject(s)
Biomass , Levulinic Acids , Phenylethyl Alcohol , Wood , Levulinic Acids/metabolism , Wood/chemistry , Wood/metabolism , Hydrolysis , Phenylethyl Alcohol/metabolism , Kluyveromyces/metabolism , Kluyveromyces/growth & development , Lignin/metabolism , Lignin/chemistry , Pinus/metabolism , Pinus/chemistry
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