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1.
Vet Parasitol ; 194(2-4): 202-6, 2013 May 20.
Article in English | MEDLINE | ID: mdl-23462251

ABSTRACT

Protection against Trichinella infections has been achieved using various parasite antigens and adjuvants. Recently, we reported that immunization of mice with an attenuated Salmonella strain displaying a 30-mer peptide (residues 210-239) from the Trichinella spiralis gp43 antigen using the ShdA autotransporter induced partial protection against T. spiralis infection. To improve the efficacy of vaccination, we used the MisL autotransporter system to display the Ts30mer peptide on the surface of Salmonella enterica ser. Typhimurium in combination with a prime-boost vaccination strategy. This vector and immunization regimen induced superior protection against T. spiralis when compared to our previously reported approach. Data presented herein showed a significant reduction in adult worm and muscle larvae burdens, high IgG titers, and increased production of intestinal mucus with entrapped adult worms. This prime-boost vaccination scheme is a suitable strategy to elicit enhanced protective immunity against T. spiralis.


Subject(s)
Antibodies, Helminth/biosynthesis , Antigens, Helminth/immunology , Antigens, Neoplasm/immunology , Salmonella/genetics , Trichinella spiralis/immunology , Trichinellosis/prevention & control , Animals , Antibodies, Helminth/genetics , Antibodies, Helminth/immunology , Antigens, Helminth/genetics , Antigens, Neoplasm/genetics , Female , Gene Expression , Intestines/parasitology , Larva , Mice , Mice, Inbred BALB C , Muscles/parasitology , Recombinant Fusion Proteins/metabolism , Salmonella/immunology , Salmonella/metabolism , Trichinella spiralis/genetics , Vaccination
2.
Rev Cubana Med Trop ; 47(2): 114-7, 1995.
Article in Spanish | MEDLINE | ID: mdl-9805080

ABSTRACT

Twenty-seven cases of Aeromonas isolated from 300 five-years children with acute diarrheal disease (EDA) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. The percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher than than 1:16. The presence of markers in Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae was demonstrated.


Subject(s)
Aeromonas/genetics , Diarrhea, Infantile/microbiology , Diarrhea/microbiology , Gram-Negative Bacterial Infections/microbiology , Acute Disease , Aeromonas/isolation & purification , Aeromonas/pathogenicity , Child, Preschool , Cuba , Humans , Infant , Phenotype
3.
Rev Cubana Med Trop ; 46(1): 13-5, 1994.
Article in Spanish | MEDLINE | ID: mdl-9768226

ABSTRACT

We present the results of the in vitro action of alpha and gamma interferons and of Intacglobin and Igegam against the 47/93/IPK (Coxsackie A9) strain isolated from the cerebrospinal fluid of a patient with epidemic neuropathy. The in vitro studies showed that the two interferons inhibited the replication of this agent; they also showed the presence of antibodies to it in the Intacglobin and Igegam. The results attained demonstrated that the use of these compounds could be effective for the treatment of this entity.


Subject(s)
Antiviral Agents/pharmacology , Enterovirus/drug effects , Interferon-alpha/pharmacology , Interferon-gamma/pharmacology , Virus Replication/drug effects , Animals , Chlorocebus aethiops , Coxsackievirus Infections/cerebrospinal fluid , Coxsackievirus Infections/epidemiology , Cuba/epidemiology , Enterovirus/physiology , Humans , Vero Cells/drug effects
4.
Rev Cubana Med Trop ; 46(3): 139-43, 1994.
Article in Spanish | MEDLINE | ID: mdl-9768251

ABSTRACT

Using the C6/36 clone of the Aedes albopictus cell line, a derived cell line was obtained capable of growing in a commercial serum-free medium facilitating this line to keep its morphologic characteristics and adhesion capacity. The growth speed rate reduction of the adapted cell line was compensated by its low maintenance cost. Its usefulness in the multiplication of dengue viruses 1 and 2 was tested; the reported line showed the same susceptibility for the 2 agents than the original cell line when the indirect fluorescence technique was applied.


Subject(s)
Cell Line , Culture Media, Serum-Free , Dengue Virus/growth & development , Cell Division , Fluorescent Antibody Technique, Indirect
5.
Rev Cubana Med Trop ; 46(3): 183-4, 1994.
Article in Spanish | MEDLINE | ID: mdl-9768261

ABSTRACT

An ultramicroELISA (UME) method was normalized for the detection of antibodies to serum human parvovirus B19. The optimum antigen concentration determined was 400 ng/ml, serum dilution was of 1:100; and the conjugate work dilution was of 1:2,000. 11 paired serum samples were also evaluated and antibodies were detected. The usefulness of the analyzed system is discussed.


Subject(s)
Antibodies, Viral/analysis , Enzyme-Linked Immunosorbent Assay/standards , Parvovirus B19, Human/immunology , Antigens, Viral , Enzyme-Linked Immunosorbent Assay/methods , Humans
6.
Rev Cubana Med Trop ; 44(3): 181-4, 1992.
Article in Spanish | MEDLINE | ID: mdl-9768212

ABSTRACT

The obtention of a clone of the cell line AP-61 (Aedes pseudoscutellaris) is reported. Details of the cloning and culture media employed are discussed. Usefulness of the clone for the multiplication of dengue 1 and 2 viruses is proved in comparison with the original line using the indirect immunofluorescence technique.


Subject(s)
Aedes/cytology , Clone Cells/virology , Dengue Virus/physiology , Virus Replication , Animals , Cell Line
7.
Rev Cubana Med Trop ; 44(3): 224-5, 1992.
Article in Spanish | MEDLINE | ID: mdl-9768222

ABSTRACT

The obtention of a diploid cell line from human lung of great concern for the virological diagnosis and research is reported. Certain aspects about its application and characterization are discussed.


Subject(s)
Cell Line , Lung/cytology , Diploidy , Embryo, Mammalian , Humans , Karyotyping , Sex Factors
8.
Rev Cubana Med Trop ; 43(2): 89-92, 1991.
Article in Spanish | MEDLINE | ID: mdl-1812536

ABSTRACT

Goat's serum was used to prepare primary cultures of fibroblast from chicken embryo and hamster kidney. Later they were inoculated the East equine encephalomyelitis virus, being compared with culture from calf's serum. The choice of goat's serum is of great interest for the development of scientific research and national economy.


Subject(s)
Culture Media , Goats/blood , Animals , Cattle/blood , Cells, Cultured , Chick Embryo , Cricetinae , Encephalitis Virus, Eastern Equine/growth & development , Fibroblasts/cytology , Kidney , Macaca mulatta , Virus Cultivation/methods
9.
Rev Cubana Med Trop ; 43(3): 156-61, 1991.
Article in Spanish | MEDLINE | ID: mdl-9768180

ABSTRACT

We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.


Subject(s)
Aedes , Dengue Virus/physiology , Virus Replication , Aedes/virology , Animals , Cell Line , Cytopathogenic Effect, Viral , Fluorescent Antibody Technique , Larva/virology , Virus Cultivation/methods
10.
Rev Cubana Med Trop ; 42(2): 188-96, 1990.
Article in Spanish | MEDLINE | ID: mdl-2089503

ABSTRACT

This paper deals with the effect of several factors, during cryopreservation, on three poikilothermic cell lines using morphology and viability as indicators. It was proved that to avoid toxicity in the suspension medium is of vital importance for the outcome of freezing. In addition, one of the systems studied and of great application in Virology does not bear long hibernations. Finally, the most adequate freezing method for this type of cells is discussed.


Subject(s)
Cell Line/cytology , Cell Line/physiology , Cryopreservation/methods , Cell Survival , Cold Temperature , Time Factors
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