ABSTRACT
Soldiers in active military service need optimal physical fitness for successfully carrying out their operations. Therefore, their health status is regularly checked by army doctors. These inspections include physical parameters such as the body-mass index (BMI), functional tests, and biochemical studies. If a medical exam reveals an individual's excess weight, further examinations are made, and corrective actions for weight lowering are initiated. The collection of urine is non-invasive and therefore attractive for frequent metabolic screening. We compared the chemical profiles of urinary samples of 146 normal weight, excess weight, and obese soldiers of the Mexican Army, using untargeted metabolomics with liquid chromatography coupled to high-resolution mass spectrometry (LC-MS). In combination with data mining, statistical and metabolic pathway analyses suggest increased S-adenosyl-L-methionine (SAM) levels and changes of amino acid metabolites as important variables for overfeeding. We will use these potential biomarkers for the ongoing metabolic monitoring of soldiers in active service. In addition, after validation of our results, we will develop biochemical screening tests that are also suitable for civil applications.
Subject(s)
Military Personnel , Overweight , Humans , Overweight/diagnosis , Obesity/diagnosis , Metabolomics/methods , Body Mass Index , Weight GainABSTRACT
El cáncer cérvico-uterino (CaCU) representa el 6% de todas las neoplasias malignas en mujeres. Evidencia epidemiológica y molecular implican al virus del papiloma humano (VPH) en la etiopatogenia del CaCU y los genotipos más frecuentemente asociados son el 16 y 18 (VPH-16 y -18). La radioterapia (RT) y la quimiorradiación (RT-QT) son las dos modalidades de tratamiento primario para el CaCU, con tasas variables de resistencia o persistencia de la enfermedad. El objetivo de este trabajo fue estudiar, por primera vez, la respuesta tumoral antes, durante y después de la RT y la RT-QT y analizar la carga viral de VPH-16 y -18 en pacientes mexicanas con CaCU. Se obtuvieron muestras de tejido tumoral en pacientes con CaCU: antes, durante y después de la RT y RT-QT, se determinó el número de copias absolutas del VPH-16 o -18 y se estudió la respuesta en relación a la carga viral por PCR en tiempo real. La disminución de la carga viral de VPH para RT-QT y RT fue de 100 y 62%, respectivamente. Se observó una mejor respuesta con la modalidad de tratamiento combinado, por lo que se sugiere que la carga viral de VPH (-16 y -18) podría ser un potencial indicador para evaluar la respuesta de ambos tratamientos en el CaCU.
Cervical cancer (CaCU) represents 6% of all malignancies in women. Molecular and epidemiological evidence relate the human papilloma virus (HPV) with the pathogenesis of the CaCU genotypes, the most frequent genotypes being 16 and 18 ( VPH-16 and -18). Radiotherapy (RT) and chemoradiotherapy (ChRT) are the two primary treatment modalities for CaCU, with variable rates of resistance or persistence of the disease. The aim of this work was to study for the first time, the tumorai response before, duríng and after RT and RT-QT and analyze HPV-16 and -18 viral load in a Mexican population with CaCU. Samples of tumor tissue were collected from patients with CaCU: before, duríng and after RT and ChRT. The absolute number of HPV-16 or -18 copies was determined, and the response related with viral load in both treatments was studíed by real-time PCR-based in fluorescence. The percentage of decrease ín HPV viral load for ChRT and RT was 100% and 62%, respectívely With ChRT, the response was better than with RTalone. HPV (-16 and -18) viral load ís suggested to be a potentíal índícator to evalúate CaCU response in both treatments.
Subject(s)
Humans , Female , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/therapy , Uterine Cervical Neoplasms/virology , Radiotherapy , Uterine Neoplasms/therapy , Uterine Neoplasms/virology , Polymerase Chain Reaction , Human papillomavirus 6 , Chemoradiotherapy , MexicoABSTRACT
SUMMARY OBJECTIVES: Recent reports place prostate cancer (PCa) as third cause of death in the World among males, in Mexico it is the leading cause of male death. In this study, we studied molecular markers and one genetic marker related in tissues with PCa and benign prostatic hyperplasia (BPH): The Mn-superoxide dismutase (Mn-SOD) gen and protein, prostate specific antigen (PSA) and 3-nitrotyrosine (3-NT). It was evaluated if various markers of oxidative stress show altered expression in prostate cancer and BPH. METHODS: 80 biopsies were obtained. The conditions to amplify and evaluate the immunoreactivity of the genes of interest (Mn-SOD and 3-NT) and the correlation between PSA and Mn-SOD immunoreactivity in prostate cancer and benign prostatic hyperplasia were standardized. RESULTS: Gene overexpression and Mn-SOD and 3-NT immunoreactivity were greater in prostate cancer with respect to the BPH group. Correlation between levels of PSA and the Mn-SOD immunoreactivity was not observed. CONCLUSIONS: The above results suggest that the parameters evaluated can be used as tumor markers making the determinations in biopsies of patients suspected of prostate cancer.
Subject(s)
Prostatic Neoplasms/genetics , Prostatic Neoplasms/metabolism , Superoxide Dismutase/biosynthesis , Superoxide Dismutase/genetics , Tyrosine/analogs & derivatives , Gene Expression Regulation, Neoplastic , Genetic Markers/genetics , Humans , Male , Tyrosine/biosynthesisABSTRACT
OBJETIVO: Recientes reportes ubican al cáncer de próstata en el tercer lugar mundial, en México es la principal causa de muerte masculina. En este estudio se evaluaron: marcadores moleculares y un marcador génico en tejidos con cáncer de próstata e hiperplasia prostática benigna (HPB): el gen de la Mn-superóxido dismutasa (Mn-SOD) y su proteína, el antígeno prostático específico (APE) y 3-nitrotirosina (3-NT).Se evaluó si existe alguna alteración en la expresión de diversos marcadores de estrés oxidativo en cáncer de próstata e hiperplasia prostática benigna.MÉTODOS: Se obtuvieron 80 biopsias. Se estandarizaron las condiciones para amplificar y evaluar la inmunorreactividad del gen de interés (Mn-SOD y 3-NT) y la correlación entre el antígeno prostático específico (APE) y la inmunorreactividad de Mn-SOD en cáncer de próstata e hiperplasia prostática benigna.RESULTADOS: La sobreexpresión del gen y la inmunorreactividad de Mn-SOD, así como la inmunorreactividad de 3-NT en cáncer de próstata fue mayor con respecto al grupo con hiperplasis prostática benigna. No se observó una correlación entre los niveles de APE y la inmunorreactividad de Mn-SOD.CONCLUSIONES: Los resultados anteriores sugieren que la determinación de los parámetros evaluados pueden ser utilizados como marcadores tumorales realizando las determinaciones en biopsias de pacientes sospechosos de cáncer de próstata(AU)
OBJECTIVES: Recent reports place prostate cancer (PCa) as third cause of death in the World among males, in Mexico it is the leading cause of male death. In this study, we studied molecular markers and one genetic marker related in tissues with PCa and benign prostatic hyperplasia(BPH) : The Mn-superoxide dismutase (Mn-SOD) gen and protein , prostate specific antigen (PSA) and 3-nitrotyrosine (3-NT). It was evaluated if various markers of oxidative stress show altered expression in prostate cancer and BPH. METHODS. 80 biopsies were obtained. The conditions to amplify and evaluate the immunoreactivity of the genes of interest (Mn-SOD and 3-NT) and the correlation between PSA and Mn-SOD immunoreactivity in prostate cancer and benign prostatic hyperplasia were standardized.RESULTS. Gene overexpression and Mn-SOD and 3-NT immunoreactivity were greater in prostate cancer with respect to the BPH group. Correlation between levels of PSA and the Mn-SOD immunoreactivity was not observed.CONCLUSIONS. The above results suggest that the parameters evaluated can be used as tumor markers making the determinations in biopsies of patients suspected of prostate cancer(AU)
Subject(s)
Humans , Male , Prostatic Hyperplasia/diagnosis , Prostatic Neoplasms/diagnosis , Reactive Oxygen Species/analysis , Prostatectomy , Prostate-Specific Antigen/analysis , /analysis , Gene Expression , Superoxide Dismutase/analysisABSTRACT
El empiema de la vesícula biliar se produce por la progresión de colecistitis aguda con obstrucción persistente de las vías biliares y su posterior infección con bacterias piógenas. los principales gérmenes implicados con Escherichia coli y klebsiella. El embarazo predispone a la manifestación de este padecimiento. Su acceso es mediante operación quirúrgica y se han informado procedimientos endoscópicos y laparoscópicos. Se comunican un caso de piocolecisto y embarazo de 20 semanas de gestación tratado con operación quirúrgica y antimicrobianos, sin que haya habido ninguna complicación obstétrica
