ABSTRACT
BACKGROUND: Plants of the genus Campomanesia belong to the family Myrtaceae and are very abundant in Cerrado areas. Teas from leaves of species of this genus are used for intestinal infections, combating obesity, stomach pathology, fever and among others. OBJECTIVE: The present study evaluated the chemical composition and antioxidant potential, cytotoxicity and genotoxicity of ethanolic extract from leaves of Campomanesia pubescens (Mart. ex DC.) O.Berg. METHOD: For the evaluation of antioxidant activity, the free radical DPPH and for determination of phenolic compounds Folin-Ciocalteau reagent were used. Identification of the substances was performed by HPLC-DAD by comparison of the retention times with standards analyzed under the same conditions and by evaluation of molecular absorption spectra in the ultraviolet and visible region. The cytotoxicity, genotoxicity were evaluated using Allium cepa bioassay. RESULTS: In the ethanolic extract 7-hydroxy-6-methyl-5-methoxyflavanone, 5,7-dihydroxy-6-methylflavanone, 5,7-dihydroxy-8-methylflavanone, 2´,4´-dihydroxy-6´-methoxychalcone, 5,7-dihydroxy-6,8- dimethylflavanone, 2´,4´-dihydroxy-5´-methyl-6´-methoxychalcone and 2´,4´-dihydroxy-3´,5´-dimethyl-6´- methoxychalcone were identified. The extract showed antioxidant activity and cytotoxic effects on cell division and increased chromosomal alterations in Allium cepa test. CONCLUSION: These results showed antioxidant activity and suggest the cytotoxic and genotoxic effects in Allium cepa of ethanolic extract obtained from the leaves of Campomanesia pubescens.
Subject(s)
Antioxidants/pharmacology , Myrtaceae/chemistry , Plant Extracts/pharmacology , Plant Leaves/chemistry , Chromatography, High Pressure Liquid , Phenols/analysis , Plant Extracts/toxicityABSTRACT
O objetivo deste estudo foi avaliar o efeito de dietas acrescidas de torta de pinhão-manso (Jatropha curcas L.) no desempenho e metabolismo de ratos Wistar. Foram utilizados 24 ratos, machos albinos (Rattus norvergicus), em fase de crescimento e com peso médio inicial 109,38±6,16g. Os animais foram pesados e distribuídos aleatoriamente em dois grupos com doze animais, constituindo os tratamentos: controle dieta comercial e dietas contendo 4,281%TPM (torta de pinhão manso). O consumo de alimento, ganho em peso e conversão alimentar dos ratos foi depreciado pela inclusão de farelo de pinhão-manso. Observou-se discreto aumento na atividade das enzimas ALT e AST hepática nos ratos alimentados com dietas acrescidas de TPM. Os valores de hemoglobinas e hematócritos encontravam-se dentro deste intervalo considerados adequados para roedores. Não foram reportados diferenças no equilíbrio eletrolítico e no metabolismo respiratório. Conclui-se que a inclusão de torta de pinhão-manso na alimentação de ratos afeta negativamente o desenvolvimento do crescimento e promove alterações deletérias hepáticas.
This study aimed to evaluate the effect of diets plus pie of Jatropha (Jatropha curcas l.) in performance and metabolism of male Wistar rats. 24 rats were used, albino males (Rattus norvegicus) in growth phase and initial average weight 109.38 ± 6, 16 g. The animals were weighed and randomly divided into two groups with 12 animals, constituting the treatments: control standard diet and diets containing 4, 281TPM. Food consumption, body weight gain and feed conversion ratio of rats has been deprecated by the inclusion of Jatropha bran. The liver enzymes ALT and AST in rats fed diets increased Jatropha. The values of hemoglobin and hematocrit were within this range considered adequate for rodents. Not been reported differences in the electrolytic balance and respiratory metabolism. It is concluded that the inclusion of Jatropha pie in the feeding of rats depresses growth and development promotes hepatic deleterious changes.
