ABSTRACT
A strain of Pseudomonas fluorescens, capable of growing on styrene as the sole C source was isolated from enrichment cultures. From the Pseudomonas cultures supplied with styrene, phenylacetic and o-hydroxyphenylacetic acids were isolated and identified, o-Hydroxyphenylacetic acid was also isolated from the cultures supplied with phenylacetate. Homogentisate 1,2-dioxygenase is induced in the cells grown either on styrene or phenylacetate or o-hydroxyphenylacetate. A pathway for styrene metabolism through the intermediary formation of phenylacetate and o-hydroxyphenylacetate which is further oxidized via homogentisate is suggested and discussed.
ABSTRACT
Two Achromobacter strains capable of utilizing 2,2-dimethylheptane or tertbutylbenzene as the sole carbon and energy source were isolated from waste-water. Pivalic acid was found in the cultures of Achromobacter A1 containing 2,2-dimethylheptane. From cultures of Achromobacter A2 in the presence of tertbutylbenzene, a diol was isolated and identified as 2,3-dihydro-2,3-dihydroxytertbutylbenzene. Evidence for meta cleavage of the aromatic ring and for accumulation of pivalic acid in the cultures was also obtained. A metabolic pathway for tertbutylbenzene is suggested.
Subject(s)
Alcaligenes/metabolism , Benzene Derivatives/metabolism , Heptanes/metabolism , Water Microbiology , Biodegradation, Environmental , Chemical Phenomena , Chemistry , Pentanoic Acids/metabolism , SewageABSTRACT
The structure of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid for the meta-cleavage product of 2,3-dihydroxybiphenyl by a Pseudomonas putida strain was demonstrated on the basis of its chemical and physicochemical properties and those of its derivatives.
Subject(s)
Biphenyl Compounds/metabolism , Pseudomonas/metabolism , Chromatography, Gas , Hydrogenation , Models, Chemical , Sorbic Acid/analogs & derivatives , Sorbic Acid/biosynthesis , Sorbic Acid/chemical synthesis , Spectrum AnalysisABSTRACT
1. 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid was isolated and identified from washed suspensions of Pseudomonas putida incubated in the presence of 2,3-dihydroxybiphenyl. 2. Benzoic acid was isolated from reaction mixtures of crude cell-free extracts incubated with 2,3-dihydroxybiphenyl. 3. The presence in the same reaction mixtures of either 4-hydroxy-2-oxovalerate or 2-hydroxypenta-2,4-dienoate was suggested by mass spectrometry. 4. The degradative pathway of biphenyl is discussed.
Subject(s)
Biphenyl Compounds/metabolism , Fatty Acids, Unsaturated/biosynthesis , Pseudomonas/metabolism , Benzoates/isolation & purification , Cell-Free System , Chromatography, Thin Layer , Keto Acids , Mass Spectrometry , Spectrophotometry, UltravioletABSTRACT
1. Two Pseudomonas strains capable of utilizing 2-phenylbutane, 3-phenylpentane and 4-phenylheptane as the sole carbon and energy source were isolated. 2. Two Nocardia strains capable of utilizing only 3-phenyldodecane as the sole carbon and energy source were isolated. 3. All the isolated strains were unable to grow on the corresponding phenylalkane-p-sulphonates. 4. From liquid cultures of Pseudomonas strains utilizing 2-phenylbutane, 2-(2,3-dihydro-2,3-dihydroxyphenyl)butane was isolated and identified. Evidence for a meta cleavage of the benzene ring was also obtained. 5. From liquid cultures of Pseudomonas strains utilizing 3-phenylpentane, 3-(2,3-dihydro-2,3-dihydroxyphenyl)pentane and 2-hydroxy-7-ethyl-6-oxonona-2,4-dienoic acid were isolated and identified. 6. Evidence for the formation of both a diol and a meta-cleavage compound was obtained from liquid cultures of both Pseudomonas strains utilizing 4-phenylheptane. 7. Liquid cultures of both Nocardia strains utilizing 3-phenyldodecane never formed a diol or a semialdehyde-related compound. 2-Phenylbutyric acid, 3-phenylvaleric acid and 4-phenylhexanoic acid were shown to be present in these cultures.
Subject(s)
Benzene Derivatives/metabolism , Nocardia/metabolism , Pseudomonas/metabolism , Alkanes/metabolism , Carbon/metabolism , Manometry , Phenylbutyrates/metabolism , Spectrophotometry , Tryptophan Oxygenase , Ultraviolet RaysABSTRACT
1. (+)-gamma-Carboxymethyl-gamma-methyl-Delta(alpha)-butenolide was isolated from resting-cell cultures of Pseudomonas desmolyticum incubated in the presence of 5-methylsalicylic acid. 2. The structure of this metabolite was deduced from physical and chemical evidence. 3. The isolated compound must be formed in an enzymic reaction since it shows optical activity. 4. The degradative pathway of 4-methylcatechol by Ps. desmolyticum is discussed.