ABSTRACT
Different strategies have been proposed to overcome maternally derived antibody (MDA) interference with canine parvovirus type 2 (CPV-2) immunisation, including intranasal vaccination, which presents some practical limitations. In the present study, the results of the oral administration of a commercial CPV-2b modified live virus (MLV) vaccine in pups with MDA are reported. The CPV-2b vaccine was orally administered to 14 6-week-old pups with a bait. Blood samples and rectal swabs were collected at different days post-vaccination (dpv) to determine CPV-2 antibody titres and DNA loads. Thirteen pups were positive to serological and virological tests after the first vaccination and one pup became positive after the second vaccine administration. The findings of this study suggest that systemic immunity against CPV-2 may be achieved by the use of an MLV CPV-2b vaccine administered orally even in the presence of MDA titres that usually interfere with vaccination.
Subject(s)
Dog Diseases/prevention & control , Parvoviridae Infections/prevention & control , Parvovirus, Canine/immunology , Viral Vaccines/administration & dosage , Administration, Oral , Animals , Dog Diseases/immunology , Dogs , Female , Male , Parvoviridae Infections/immunology , Parvoviridae Infections/veterinary , Vaccination/veterinary , Viral Vaccines/immunologyABSTRACT
Hepatitis A virus (HAV) was detected in a batch of imported non-packaged frozen redcurrants purchased in a Bari grocery. Sequence and phylogenetic analysis showed the HAV strain clustered tightly with the HAV strain from the 2013 Italian epidemic, providing additional evidence that frozen redcurrants were the main vehicle of the HAV outbreak.
Subject(s)
Fruit/virology , Hepatitis A virus/isolation & purification , Hepatitis A/virology , Ribes/virology , Disease Outbreaks , Fruit/economics , Hepatitis A/epidemiology , Hepatitis A virus/classification , Hepatitis A virus/genetics , Humans , Italy/epidemiology , Molecular Sequence Data , Phylogeny , Poland , RNA, Viral/geneticsABSTRACT
Global surveillance for norovirus identified in 2012 the emergence of a novel pandemic GII.4 variant, termed Sydney 2012. In Italy, the novel pandemic variant was identified as early as November 2011 but became predominant only in the winter season 2012-2013. Upon sequencing and comparison with strains of global origin, the early Sydney 2012 strains were found to differ from those spreading in 2012-2013 in the capsid (ORF2) putative epitopes B, C and D, segregating into a distinct phylogenetic clade. At least three residues (333, 340 and 393, in epitopes B, C and D, respectively) of the VP1 varied among Sydney 2012 strains of different clades. These findings suggest that the spread of the pandemic variant in Italy during the winter season 2012-2013 was due to the introduction of strains distinct from those circulating at low frequency in the former winter season and that similar strains were also circulating elsewhere worldwide.
Subject(s)
Capsid Proteins/genetics , Gastroenteritis/virology , Mutation , Norovirus/genetics , Amino Acid Sequence , Capsid Proteins/metabolism , Gastroenteritis/epidemiology , Humans , Italy/epidemiology , Molecular Sequence Data , Norovirus/classification , Norovirus/isolation & purification , Norovirus/physiology , Pandemics , Phylogeny , SeasonsABSTRACT
During 2012, a novel pandemic GII.4 norovirus variant, Sydney 2012, emerged worldwide. A signature of the variant was a GII.Pe ORF1, in association with GII.4 Apeldoorn 2008-like ORF2-ORF3 genes. We report the detection of recombinant GII.4 Sydney 2012 strains, possessing the ORF1 gene of the former pandemic variant New Orleans 2009.
Subject(s)
Caliciviridae Infections/virology , Norovirus/classification , Norovirus/genetics , Recombination, Genetic , Caliciviridae Infections/epidemiology , Child , Child, Preschool , Humans , Molecular Sequence Data , Norovirus/isolation & purification , Open Reading Frames , Pandemics , RNA, Viral/genetics , Sequence Analysis, DNAABSTRACT
Infection by a novel canine astrovirus was associated with gastroenteritis in two dogs. The virus displayed 70.3 to 73.9% amino acid identity to other canine astroviruses in the full-length capsid. Specific antibodies were detected in the convalescent-phase sera of the dogs, indicating seroconversion. Also, the virus appeared weakly related antigenically to the prototype canine astrovirus isolate ITA/2008/Bari.
