ABSTRACT
The present work reports the detection of the first case of nosocomial Klebsiella oxytoca producing class A carbapenemase KPC-2 in Brazil. The isolate KPN106 carried a 65-kb IncW-type plasmid that harbors the blaKPC gene and Tn4401b. Moreover, we detected the presence of a class 1 integron containing a new allele, arr-8, followed by a 5'-truncated dhfrIIIc gene. In view of the recent results, we emphasize the high variability of the bacterial and genetic hosts of this resistance determinant.
Subject(s)
Genes, Bacterial , Klebsiella oxytoca/enzymology , beta-Lactamases/genetics , Aged , Alleles , Brazil , DNA Transposable Elements , DNA, Bacterial/genetics , Drug Resistance, Bacterial , Fatal Outcome , Female , Genetic Variation , Humans , Integrons , Klebsiella Infections/diagnosis , Klebsiella Infections/drug therapy , Klebsiella oxytoca/drug effects , Klebsiella oxytoca/genetics , Klebsiella oxytoca/isolation & purification , Microbial Sensitivity Tests , Plasmids/genetics , Plasmids/metabolism , Polymyxin B/pharmacology , Rifampin/pharmacologyABSTRACT
This work reports the identification of the first case of a KΡC-2-producing Pseudomonas putida isolate (PP36) in Brazil. The PP36 isolate was resistant to all the antimicrobials tested except polymyxin B. In addition to the discovered bla(KPC-2) gene, genetic analysis showed the presence of a class 1 integron containing the dhfrXVb gene and the new allele arr-6, which codes for resistance to rifampin. These elements were found in an IncFI 65-kb plasmid.