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Anal Biochem ; 169(1): 104-8, 1988 Feb 15.
Article in English | MEDLINE | ID: mdl-3369676

ABSTRACT

An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated on an automated DNA synthesizer, contains high levels of attached DNA, and has superior mechanical properties. It should be generally useful for affinity chromatography of DNA binding proteins.


Subject(s)
DNA-Binding Proteins/isolation & purification , Oligonucleotides , Base Sequence , Chromatography, Affinity , Oligonucleotides/chemical synthesis , Polytetrafluoroethylene
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