ABSTRACT
BACKGROUND AND OBJECTIVE: Epigenetic events, as the DNA methylation, may be related to development of inflammatory diseases. Due to the important role of host's response in the pathogenesis of periodontitis, the purpose of the present study was to investigate the methylation profile of genes related to immune response in gingival tissues from patients with generalized periodontitis (GP) compared to healthy individuals. METHODS: Gingival tissues were collected from 20 individuals with GP and 20 healthy individuals. Genomic DNA was extracted and submitted to enzymatic digestions. An initial screening using a panel of genes involved with the response immune was performed in pools containing six samples of each group. Genes that presented different levels of methylation between the groups were selected for individual assays for validation. RESULTS: The array results showed an unmethylated profile in the majority of genes evaluated in both groups. MALT1, LTB, and STAT5 genes presented a profile of partial methylation in the control compared with GP group. Validation individual assays using a larger number of samples (n = 20, each group) confirmed the hypomethylation of STAT5 in the GP group compared with control group (P < .001). CONCLUSION: Generalized periodontitis is associated with hypomethylation of the STAT5 gene. Further studies are necessary to evaluate the functional impact these findings.
Subject(s)
DNA Methylation , Periodontitis/genetics , Periodontitis/immunology , STAT5 Transcription Factor/genetics , Case-Control Studies , Gingiva , Humans , Promoter Regions, GeneticABSTRACT
OBJECTIVES: The odontogenic keratocyst (OKC) is an aggressive odontogenic cyst that has a high recurrence rate. Apart from PTCH1 mutations, few molecular alterations are described in OKCs. Low expression of microRNAs (miRNAs) miR-15a and/or miR-16-1 in association with increased expression of their target, Bcl-2, have been previously found in OKC. In humans, MIR15A and MIR16-1 are clustered at chromosome position 13 q14, and loss of heterozygosity (LOH) at this locus occurs in different tumors. We aimed to determine whether deletion at 13 q14 is a potential mechanism leading to miR-15a/16-1 aberrant expression in OKC. METHODS: Genomic DNA was extracted from 15 formalin-fixed, paraffin-embedded microdissected OKC cases. The polymorphic DNA markers D13S272 and D13S273 on chromosome 13 q14.3, around MIR15A/MIR16-1, were amplified by polymerase chain reaction. LOH was examined by capillary electrophoresis DNA-fragment analysis. RESULTS: The D13S272 marker had no LOH in 12 informative cases, whereas 2 out of 9 informative cases (22%) had LOH at the D13S273 marker. CONCLUSIONS: An LOH event at MIR15A/MIR16-1 locus is not common in OKC. The mechanism underlying the regulation of miR-15a and miR-16-1 expression in OKC remains to be determined.
Subject(s)
Loss of Heterozygosity , MicroRNAs/genetics , Odontogenic Cysts/genetics , Odontogenic Tumors/genetics , Proto-Oncogene Proteins c-bcl-2/genetics , Adolescent , Adult , Chromosomes, Human, Pair 13 , DNA Fragmentation , Electrophoresis, Capillary , Female , Humans , Male , Middle Aged , Odontogenic Cysts/pathology , Odontogenic Tumors/pathology , Polymerase Chain ReactionABSTRACT
OBJECTIVE: Salivary gland neoplasms pathogenesis has not been well established. DNA methylation occurs when methyl groups are added to cytosine nucleotides in specific areas of the gene by the enzyme DNA methyltransferase (DNMT). This chemical modification can alter gene expression without altering DNA sequence. While DNMT3a is mostly involved in de novo methylation, DNMT1 acts as a maintenance methyltransferase. We aimed to investigate the immunoexpression of DNMT3a and DNMT1 in minor salivary gland neoplasms, comparing it with normal tissue. MATERIAL: Forty-four formalin-fixed and paraffin-embedded samples of pleomorphic adenoma, adenoid cystic carcinoma, mucoepidermoid carcinoma and polymorphous low-grade adenocarcinoma were included in the study. The DNMT1 and DNMT3a proteins were identified by using a highly sensitive polymer-based system. RESULTS: Positive nuclear and cytoplasmic labeling for DNMT1 was observed in all samples, including the controls. Positive nuclear labeling for DNMT3a was found only in few neoplasms: 1 pleomorphic adenoma (9.0%), 2 adenoid cystic carcinoma (16.6%) and 1 mucoepidermoid (9.0%) cases. CONCLUSION: Our results were not able to demonstrate a clear correlation between DNMT1 and DNMT3a immunoexpression and salivary gland neoplasms development.
Subject(s)
DNA (Cytosine-5-)-Methyltransferases/biosynthesis , Salivary Gland Neoplasms/metabolism , Adolescent , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/metabolism , DNA (Cytosine-5-)-Methyltransferase 1 , DNA Methylation , DNA Methyltransferase 3A , Female , Humans , Immunohistochemistry , Male , Middle Aged , Salivary Glands, Minor/metabolism , Salivary Glands, Minor/pathology , Young AdultABSTRACT
Introduction: This study was designed to investigate the effect of oral HSV-1 shedding on the survival of allogeneichematopoietic stem cell transplanted (allo-HSCT) patients.Methods: One hundred nineteen allo-HSCT patients were included in the study and divided in three groups: beforetransplant, 100 days after transplant and 1 year of allo-HSCT. Healthy volunteers matched by age and genderwere also selected. Oral swabs were performed and the nested PCR was used to detect HSV-1 presence in the oralmucosa. In statistical analysis, chi-square test was used to test the distribution of HSV1 shedding among the threegroups. Time to death after allo-HSCT was displayed by means of the Kaplan-Meier method and the results werecompared by the log-rank test. Cox proportional hazards multivariate model was used to evaluate the survival.Results: We observed that HSV-1 shedding was similar at different points after allo-HSCT. However, HSV-1 sheddingbefore allo-HSCT was associated with worst survival rates after allo-HSCT in multivariate analysis.Conclusion: Our data demonstrates that HSV-1 shedding in oral mucosa before transplant is associated with worstsurvival rate of allo-HSCT patients (AU)
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Hematopoietic Stem Cell Transplantation/mortality , Herpesvirus 1, Human/isolation & purification , Mouth Mucosa/virology , Survival RateABSTRACT
Describimos un caso clínico de varices en la mucosa yugal que fue tratado con escleroterapia. El agente esclerosante usado fue oleato de monoetanolamina. Después de la tercera sesión las varices desparecieron y la paciente mejoro
We reported a case of varicosities in the buccal mucosa treated with sclerotherapy. The sclerosant agent used was the monoethanolamineoleate. After three sessions the lesions disappeared and the patient is follow-up
