Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Proteomics ; 12(17): 2682-93, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22761183

ABSTRACT

It is well known that the activation of mast cells due to the binding of mastoparan to the G(α) subunit of trimeric G proteins involves exocytosis regulation. However, experimental evidence in the literature indicates that mastoparan can also activate certain regulatory targets of exocytosis at the level of the mast cell endosomal membranes that have not yet been identified. Therefore, the aim of the present investigation was the proteomic identification of these targets. To achieve these objectives, mast cells were activated by the peptide Protopolybia MP-III, and the proteins of the endosomal membranes were converted to proteoliposomes using sonication. Proteins were separated from one another by affinity chromatography using proteoliposomes as analytes and Protopolybia MP III-immobilized Sepharose 4B resin as the ligand. This experimental approach, which used SDS-PAGE, in-gel trypsin digestion and proteomic analysis, permitted the identification of five endosomal proteins: Rho GTPase Cdc 42 and exocyst complex component 7 as components of the Ca(2+) -independent FcεRI-mediated exocytosis pathway, synaptosomal-associated protein 29, and GTP-binding protein Rab3D as components of the Ca(2+) -dependent FcεRI-mediated exocytosis pathway and Ras-related protein M-Ras, a protein that is related to the mediation of cell shaping and proliferation following exocytosis. The identification of the five proteins as targets of mastoparans may contribute in the near future to the use of this family of peptides as novel tools for dissecting the mechanism of exocytosis in mast cells.


Subject(s)
Endosomes/metabolism , GTP-Binding Proteins/metabolism , Mast Cells/metabolism , Peptides/metabolism , Proteomics , Wasp Venoms/metabolism , Amino Acid Sequence , Animals , Cell Degranulation , Chromatography, Affinity , Electrophoresis, Polyacrylamide Gel , Endosomes/enzymology , Exocytosis , GTP-Binding Proteins/chemistry , GTP-Binding Proteins/isolation & purification , Intercellular Signaling Peptides and Proteins , Male , Mass Spectrometry , Mast Cells/cytology , Molecular Sequence Data , Peptides/chemical synthesis , Peptides/chemistry , Rats , Wasp Venoms/chemical synthesis , Wasp Venoms/chemistry , Wasps/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...