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1.
J Med Virol ; 92(2): 260-262, 2020 02.
Article in English | MEDLINE | ID: mdl-31579928

ABSTRACT

Serological testing for immunoglobulin M (IgM) antibodies to Hepatitis E virus (HEV) is useful for diagnosing acute hepatitis E. Our aim was to evaluate the performance of chemiluminescence immunoassay (HEV IgM; Vircell, Granada, Spain) in comparison with a manual enzyme immunoassay (Anti-HEV virus; Wantai, Beijing, China) for anti-HEV IgM detection. Seventeen HEV-RNA positive and 69 HEV-RNA negative sera were tested by both assays. Virclia sensitivity was 94.1%, whereas that of the enzyme immunosorbent assay (EIA) was 76.5%; specificity was 98.5% and 97.1% for Virclia and EIA, respectively. Differences between both assays were not statistically significant. Virclia HEV IgM showed excellent performance and can be a good alternative to conventional enzyme immunoassays for the detection of IgM against HEV.


Subject(s)
Antibodies, Viral/blood , Hepatitis E/blood , Hepatitis E/diagnosis , Hepatitis E/immunology , Immunoenzyme Techniques/methods , Luminescent Measurements/methods , Hepatitis E virus/immunology , Humans , Immunoassay/methods , Immunoglobulin G/blood , Immunoglobulin M/blood , Sensitivity and Specificity , Seroconversion , Serologic Tests/methods
2.
Infection ; 47(5): 739-747, 2019 Oct.
Article in English | MEDLINE | ID: mdl-30888587

ABSTRACT

OBJECTIVES: To investigate the proportion of asymptomatic infection among blood donors in a region endemic for Leishmania; and to ascertain epidemiological and genetic factors associated with this condition. METHODS: We studied 1260 blood donors in the Province of Granada in the Southern Spain. After obtaining informed consent in each participant, a poll about habits, housing and contact with animals were carried out. Blood samples were obtained for determining antileishmanial antibodies and a PCR assay. HLA typing was performed in a randomly sample among the donors with positive serology. RESULTS: We have found that L. infantum antibodies were present in 7.9% of blood donors and DNA in blood was detected in 2.5% of donors. There was no concordance between both determinations, except in one patient. Taking into consideration both techniques, 129 participants were considered to have asymptomatic Leishmania infection. No participant in this study developed clinical leishmaniasis during a follow-up period of 2 years. HLA were typed in 51 donors. Asymptomatic Leishmania infection might be associated with certain HLA antigens. A multivariate analysis was done with the variables obtained through the participants' interview. The contact with livestock (goats, pigs, and sheep), but not dogs, either at home or in the environment, was significantly and independently associated with asymptomatic leishmania infection. CONCLUSIONS: Asymptomatic leishmanial infection among blood donors is frequent in the Granada Province, south of Spain. The presence of livestock in this region is related to this infection, perhaps influencing vector density of this disease. Some HLA genes might be associated with asymptomatic leishmanial state.


Subject(s)
Asymptomatic Infections/epidemiology , Blood Donors , Leishmaniasis/blood , Leishmaniasis/epidemiology , Adolescent , Adult , Aged , Animals , Antibodies, Protozoan/blood , DNA, Protozoan/blood , Endemic Diseases , Female , HLA Antigens/genetics , Humans , Leishmania infantum/genetics , Livestock/parasitology , Male , Middle Aged , Spain/epidemiology , Young Adult
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