ABSTRACT
The difference in sensitivity of UR/UT toward Lubrol WX permeabilization treatment of stationary phase E. coli cells is not uniquely related to nitrogen availability during cellular growth. The sensitivity of UR/UT to detergent treatment appears to be related to differences in the balance between fermentative and oxidative glucose metabolism. The possible occurrence of a third cycle in the glutamine synthetase regulatory cascade mechanism is considered.
Subject(s)
Escherichia coli/enzymology , Nucleotidyltransferases/metabolism , Detergents/pharmacology , Enzyme Stability , Escherichia coli/drug effects , Escherichia coli/growth & development , Kinetics , PII Nitrogen Regulatory Proteins , Polyethylene Glycols/pharmacologySubject(s)
Escherichia coli/enzymology , Glutamate-Ammonia Ligase/metabolism , Nucleotidyltransferases/antagonists & inhibitors , UDPglucose-Hexose-1-Phosphate Uridylyltransferase/antagonists & inhibitors , Uracil Nucleotides/metabolism , Uridine Monophosphate/metabolism , Cell Membrane Permeability , Detergents/pharmacology , Escherichia coli/drug effects , Kinetics , Polyethylene Glycols/pharmacologyABSTRACT
The specific inactivation of the uridylylation cycle of glutamine synthetase regulatory system occurring when E. coliW grown with limited nitrogen supply is subjected to permeabilization by Lubrol WX, is not strictly related to the nitrogen starvation at cell harvesting. Evidences indicating that the sensitivity of uridylylremoving-uridylyltransferase enzyme complex to detergent treatment is affected by both rate of growth and cellular yield of the culture, are presented.
