Endoparasites of Crotalus tzabcan (Serpentes: Viperidae), with a checklist in rattlesnakes.

The helminth and pentastomid fauna of 50 specimens of Crotalus tzabcan from the Yucatán Peninsula, Mexico is documented. The examination revealed the presence of three nematode species (Hastospiculum onchocercum, Hexametra boddaertii, and Travassosascaris araujoi), and one pentastomid (Porocephalus crotali). The threee nematode species had the same prevalence (2%), while the pentastomid had a higher prevalence (8%). The pentastomid P. crotali was the most abundant and intense parasite, although it was only found in four snake hosts. Crotalus tzabcan acts as definitive host for the adult helminths and pentastomids, with rodents as the probable intermediate hosts. This work represents the first systematic survey on the parasitic helminth and pentastomid fauna of C. tzabcan, and includes four new geographical records. Additionally, a checklist of helminths and pentastomids reported for Crotalus and Sistrurus is provided. To date, a total of 32 helminth and 7 pentastomid species have been recorded as parasites of rattlesnakes. Nematoda possessed the highest species richness. The genera with the highest number of host species were Mesocestoides and Hexametra, followed by Kalicephalus. The rattlesnake species with the highest number of reported parasites was C. durissus (18 nematodes and 2 pentastomids).

Occurrence of Amblyomma dissimile on wild crocodylians in southern Mexico.

Ticks are common ectoparasites of amphibians and reptiles but very few reports of such parasites on crocodylians exist worldwide. Herein, we report the first detailed observations of Amblyomma dissimile Koch, 1844 on the 3 crocodylian species present in Mexico, with the first report of tick parasitism on Crocodylus acutus and the second on Caiman crocodilus chiapasius. This is also the first report of A. dissimile in the state of Quintana Roo. Proportions of infested individuals found in this study ranged from 0.51 to 1.96%, suggesting that tick parasitism in crocodylians is likely opportunistic and occurs when individuals leave the water for terrestrial activities. Tick parasitism does not represent a major threat to crocodylians. The increasing habitat destruction/fragmentation and cattle expansion in southeastern Mexico, however, could increase tick populations and trigger tick parasitism and tick-borne diseases in herpetofauna and other vertebrates, including humans. Thus, studies are needed to better understand these relationships.

Oral and cloacal microflora of wild crocodiles Crocodylus acutus and C. moreletii in the Mexican Caribbean.

Bacterial cultures and chemical analyses were performed from cloacal and oral swabs taken from 43 American crocodiles Crocodylus acutus and 28 Morelet's crocodiles C. moreletii captured in Quintana Roo State, Mexico. We recovered 47 bacterial species (28 genera and 14 families) from all samples with 51.1% of these belonging to the family Enterobacteriaceae. Fourteen species (29.8%) were detected in both crocodile species and 18 (38.3%) and 15 (31.9%) species were only detected in American and Morelet's crocodiles, respectively. We recovered 35 bacterial species from all oral samples, of which 9 (25.8%) were detected in both crocodile species. From all cloacal samples, we recovered 21 bacterial species, of which 8 (38.1%) were detected in both crocodile species. The most commonly isolated bacteria in cloacal samples were Aeromonas hydrophila and Escherichia coli, whereas in oral samples the most common bacteria were A. hydrophila and Arcanobacterium pyogenes. The bacteria isolated represent a potential threat to crocodile health during conditions of stress and a threat to human health through crocodile bites, crocodile meat consumption or carrying out activities in crocodile habitat. We especially warn about the presence of Salmonella arizonae and S. typhi, which cause enteritis and septicemia in crocodiles and salmonellosis and typhoid fever in humans. The risk of bacterial contamination from crocodiles to humans could increase in the future because of the accelerated destruction of crocodile habitat, which could lead to an augmentation of human-crocodile interactions. Information on bacterial diversity reported here could help in the choice of antibacterial products in case of infections that are of crocodile origin.