ABSTRACT
In pigs, luteolytic sensitivity to PGF-2α (=LS) is delayed until d 13 of the estrous cycle. While the control of LS is unknown, it is temporally associated with macrophage (MAC; which secretes tumor necrosis factor [TNF]-α) infiltration into the corpora lutea (CL), and previous studies have shown that TNF-α induces LS in porcine luteal cells (LCs) in culture. This study was designed to explore the control of LS by CL macrophage (CL MAC)/TNF-α by progesterone (P4), and to examine the hypothesis that P4 acting via the genomic P4 receptor (PGR) inhibits CL MAC TNF-α and thus plays a key role in regulating LS during the pig estrous cycle. In experiment 1, the effects of LCs on CL MAC cytokine/TNF-α mRNA expression in co-culture were examined (MID cycle; ~d 7-12; no LS); results showed that LC was inhibitory to cytokine/TNF-α. In experiment 2, the effects of P4 or R5020 (PGR-agonist) on CL MAC cytokine/TNF-α mRNA expression were examined (MID cycle; ~d 7-12; no LS); results showed that both P4 and R5020 dose-dependently inhibited TNF-α. In experiment 3, CL MACs were isolated from CL at MID (~d 7-12; no LS) and LATE (~d 13-18; + LS) cycle, and TNF-α/PGR mRNA measured. Results indicated that while TNF-α mRNA was 4.2-fold greater in CL MACs from LATE vs MID cycle, PGR mRNA was 4.5-fold greater in CL MACs from MID vs LATE cycle. These data support our hypothesis and suggest that progesterone, acting via PGR, plays a critical physiological role in the control of TNF-α production by CL MACs and LS during the pig estrous cycle.
Subject(s)
Cytokines/metabolism , Macrophages/drug effects , Progesterone/pharmacology , Receptors, Progesterone/metabolism , Swine , Tumor Necrosis Factor-alpha/metabolism , Animals , Cells, Cultured , Cytokines/genetics , Dose-Response Relationship, Drug , Gene Expression Regulation/drug effects , Genomics , Macrophages/metabolism , Progesterone/administration & dosage , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Tumor Necrosis Factor-alpha/geneticsABSTRACT
The human T cell lymphotropic/leukaemia virus type I (HTLV-I) causes HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The multidrug resistance associated protein 1 (ABCC1) plays multiple functions in physiopathologic responses. The expression and activity of ABCC1 was studied in T lymphocytes from uninfected and HTLV-I-infected individuals (both asymptomatic and symptomatic/HAM/TSP). ABCC1 expression and activity was reduced to nearly half in T lymphocytes from infected patients compared to control lymphocytes. Only 51.6% of CD4(+) cells from HAM/TSP patients expressed ABCC1 whereas this was seen in 60.3% from asymptomatic individuals, compared to an expression of around 86% in controls. Our results suggest that ABCC1 is negatively regulated in HTVL-I infection, supplying a novel target to investigate the pathogenesis of HTLV-I.
Subject(s)
CD4-Positive T-Lymphocytes/virology , HTLV-I Infections/metabolism , Multidrug Resistance-Associated Proteins/biosynthesis , Paraparesis, Tropical Spastic/metabolism , Adult , Aged , Blotting, Western , CD4-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/virology , Female , Flow Cytometry , Human T-lymphotropic virus 1 , Humans , Male , Middle Aged , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Os autores descrevem a técnica de anastomose mecânica após ressecçöes anteriores e reconstruçöes do trânsito de cirurgias de Hartmann. Analisam as complicaçöes possíveis, comparando-as com os resultados da literatura consultada. Relatam as experiências do Serviço com o procedimento
Subject(s)
Adult , Middle Aged , Humans , Male , Female , Rectal Neoplasms/surgery , Rectum/surgery , Sigmoid Neoplasms/surgery , Surgical StaplersABSTRACT
Apresenta-se um caso de Teratoma de Mediastino, em paciente jovem do sexo feminino com sintomatologia escassa a despeito de volumosa massa tumoral que exibia. Tecem-se comentários sobre a incidência, o diagnóstico, o tratamento, além de breve revisäo da literatura. Conclue-se pela ressecçäo cirúrgica como a melhor forma de tratamento para esta entidade, e pela radioterapia pós-operatória nos casos de ressecçöes parciais do tumor
