Visual Sensor Networks for Indoor Real-Time Surveillance and Tracking of Multiple Targets.

The recent trend toward the development of IoT architectures has entailed the transformation of the standard camera networks into smart multi-device systems capable of acquiring, elaborating, and exchanging data and, often, dynamically adapting to the environment. Along this line, this work proposes a novel distributed solution that guarantees the real-time monitoring of 3D indoor structured areas and also the tracking of multiple targets, by employing a heterogeneous visual sensor network composed of both fixed and Pan-Tilt-Zoom (PTZ) cameras. The fulfillment of the twofold mentioned goal was ensured through the implementation of a distributed game-theory-based algorithm, aiming at optimizing the controllable parameters of the PTZ devices. The proposed solution is able to deal with the possible conflicting requirements of high tracking precision and maximum coverage of the surveilled area. Extensive numerical simulations in realistic scenarios validated the effectiveness of the outlined strategy.

Multi-band MEG signatures of BOLD connectivity reorganization during visuospatial attention.

The functional architecture of the resting brain, as measured with the blood oxygenation level-dependent functional connectivity (BOLD-FC), is slightly modified during task performance. In previous work, we reported behaviorally relevant BOLD-FC modulations between visual and dorsal attention regions when subjects performed a visuospatial attention task as compared to central fixation (Spadone et al., 2015). Here we use magnetoencephalography (MEG) in the same group of subjects to identify the electrophysiological correlates of the BOLD-FC modulation found in our previous work. While BOLD-FC topography, separately at rest and during visual attention, corresponded to neuromagnetic Band-Limited Power (BLP) correlation in the alpha and beta bands (8-30 Hz), BOLD-FC modulations evoked by performing the visual attention task (Spadone et al. 2015) did not match any specific oscillatory band BLP modulation. Conversely, following the application of an orthogonal spatial decomposition that identifies common inter-subject co-variations, we found that attention-rest BOLD-FC modulations were recapitulated by multi-spectral BLP-FC components. Notably, individual variability of alpha connectivity between Frontal Eye Fields and visual occipital regions, jointly with decreased interaction in the Visual network, correlated with visual discrimination accuracy. In summary, task-rest BOLD connectivity modulations match multi-spectral MEG BLP connectivity.

Study and Development of a Fluorescence Based Sensor System for Monitoring Oxygen in Wine Production: The WOW Project.

The importance of oxygen in the winemaking process is widely known, as it affects the chemical aspects and therefore the organoleptic characteristics of the final product. Hence, it is evident the usefulness of a continuous and real-time measurements of the levels of oxygen in the various stages of the winemaking process, both for monitoring and for control. The WOW project (Deployment of WSAN technology for monitoring Oxygen in Wine products) has focused on the design and the development of an innovative device for monitoring the oxygen levels in wine. This system is based on the use of an optical fiber to measure the luminescent lifetime variation of a reference metal/porphyrin complex, which decays in presence of oxygen. The developed technology results in a high sensitivity and low cost sensor head that can be employed for measuring the dissolved oxygen levels at several points inside a wine fermentation or aging tank. This system can be complemented with dynamic modeling techniques to provide predictive behavior of the nutrient evolution in space and time given few sampled measuring points, for both process monitoring and control purposes. The experimental validation of the technology has been first performed in a controlled laboratory setup to attain calibration and study sensitivity with respect to different photo-luminescent compounds and alcoholic or non-alcoholic solutions, and then in an actual case study during a measurement campaign at a renown Italian winery.

Nonstationary multiscale turbulence simulation based on local PCA.

Turbulence simulation methods are of fundamental importance for evaluating the performance of control strategies for Adaptive Optics (AO) systems. In order to obtain a reliable evaluation of the performance a statistically accurate turbulence simulation method has to be used. This work generalizes a previously proposed method for turbulence simulation based on the use of a multiscale stochastic model. The main contributions of this work are: first, a multiresolution local PCA representation is considered. In typical operating conditions, the computational load for turbulence simulation is reduced approximately by a factor of 4, with respect to the previously proposed method, by means of this PCA representation. Second, thanks to a different low resolution method, based on a moving average model, the wind velocity can be in any direction (not necessarily that of the spatial axes). Finally, this paper extends the simulation procedure to generate, if needed, turbulence samples by using a more general model than that of the frozen flow hypothesis.

Preservation of micro-architecture and angiogenic potential in a pulmonary acellular matrix obtained using intermittent intra-tracheal flow of detergent enzymatic treatment.

Tissue engineering of autologous lung tissue aims to become a therapeutic alternative to transplantation. Efforts published so far in creating scaffolds have used harsh decellularization techniques that damage the extracellular matrix (ECM), deplete its components and take up to 5 weeks to perform. The aim of this study was to create a lung natural acellular scaffold using a method that will reduce the time of production and better preserve scaffold architecture and ECM components. Decellularization of rat lungs via the intratracheal route removed most of the nuclear material when compared to the other entry points. An intermittent inflation approach that mimics lung respiration yielded an acellular scaffold in a shorter time with an improved preservation of pulmonary micro-architecture. Electron microscopy demonstrated the maintenance of an intact alveolar network, with no evidence of collapse or tearing. Pulsatile dye injection via the vasculature indicated an intact capillary network in the scaffold. Morphometry analysis demonstrated a significant increase in alveolar fractional volume, with alveolar size analysis confirming that alveolar dimensions were maintained. Biomechanical testing of the scaffolds indicated an increase in resistance and elastance when compared to fresh lungs. Staining and quantification for ECM components showed a presence of collagen, elastin, GAG and laminin. The intratracheal intermittent decellularization methodology could be translated to sheep lungs, demonstrating a preservation of ECM components, alveolar and vascular architecture. Decellularization treatment and methodology preserves lung architecture and ECM whilst reducing the production time to 3 h. Cell seeding and in vivo experiments are necessary to proceed towards clinical translation.

Multiscale phase screen synthesis based on local principal component analysis.

Motivated by the increasing importance of adaptive optics (AO) systems for improving the real resolution of large ground telescopes, and by the need of testing the AO system performance in realistic working conditions, in this paper we address the problem of simulating the turbulence effect on ground telescope observations at high resolution. The procedure presented here generalizes the multiscale stochastic approach introduced in our earlier paper [Appl. Opt. 50, 4124 (2011)], with respect to the previous solution, a relevant computational time reduction is obtained by exploiting a local spatial principal component analysis (PCA) representation of the turbulence. Furthermore, the turbulence at low resolution is modeled as a moving average (MA) process, while previously [Appl. Opt. 50, 4124 (2011)] the wind velocity was restricted to be directed along one of the two spatial axes, the use of such MA model allows the turbulence to evolve indifferently in all the directions. In our simulations, the proposed procedure reproduces the theoretical statistical characteristics of the turbulent phase with good accuracy.

Amniotic fluid stem cells restore the muscle cell niche in a HSA-Cre, Smn(F7/F7) mouse model.

Mutations in the survival of motor neuron gene (SMN1) are responsible for spinal muscular atrophy, a fatal neuromuscular disorder. Mice carrying a homozygous deletion of Smn exon 7 directed to skeletal muscle (HSA-Cre, Smn(F7/F7) mice) present clinical features of human muscular dystrophies for which new therapeutic approaches are highly warranted. Herein we demonstrate that tail vein transplantation of mouse amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. Second, after cardiotoxin injury of the Tibialis Anterior, only AFS-transplanted mice efficiently regenerate. Most importantly, secondary transplants of satellite cells (SCs) derived from treated mice show that AFS cells integrate into the muscle stem cell compartment and have long-term muscle regeneration capacity indistinguishable from that of wild-type-derived SC. This is the first study demonstrating the functional and stable integration of AFS cells into the skeletal muscle, highlighting their value as cell source for the treatment of muscular dystrophies.

Multiscale stochastic approach for phase screens synthesis.

Simulating the turbulence effect on ground telescope observations is of fundamental importance for the design and test of suitable control algorithms for adaptive optics systems. In this paper we propose a multiscale approach for efficiently synthesizing turbulent phases at very high resolution. First, the turbulence is simulated at low resolution, taking advantage of a previously developed method for generating phase screens [J. Opt. Soc. Am. A 25, 515 (2008)]. Then, high-resolution phase screens are obtained as the output of a multiscale linear stochastic system. The multiscale approach significantly improves the computational efficiency of turbulence simulation with respect to recently developed methods [Opt. Express 14, 988 (2006)] [J. Opt. Soc. Am. A 25, 515 (2008)] [J. Opt. Soc. Am. A 25, 463 (2008)]. Furthermore, the proposed procedure ensures good accuracy in reproducing the statistical characteristics of the turbulent phase.

The "Wireless Sensor Networks for City-Wide Ambient Intelligence (WISE-WAI)" Project.

This paper gives a detailed technical overview of some of the activities carried out in the context of the "Wireless Sensor networks for city-Wide Ambient Intelligence (WISE-WAI)" project, funded by the Cassa di Risparmio di Padova e Rovigo Foundation, Italy. The main aim of the project is to demonstrate the feasibility of large-scale wireless sensor network deployments, whereby tiny objects integrating one or more environmental sensors (humidity, temperature, light intensity), a microcontroller and a wireless transceiver are deployed over a large area, which in this case involves the buildings of the Department of Information Engineering at the University of Padova. We will describe how the network is organized to provide full-scale automated functions, and which services and applications it is configured to provide. These applications include long-term environmental monitoring, alarm event detection and propagation, single-sensor interrogation, localization and tracking of objects, assisted navigation, as well as fast data dissemination services to be used, e.g., to rapidly re-program all sensors over-the-air. The organization of such a large testbed requires notable efforts in terms of communication protocols and strategies, whose design must pursue scalability, energy efficiency (while sensors are connected through USB cables for logging and debugging purposes, most of them will be battery-operated), as well as the capability to support applications with diverse requirements. These efforts, the description of a subset of the results obtained so far, and of the final objectives to be met are the scope of the present paper.

Stochastic realization approach to the efficient simulation of phase screens.

The phase screen method is a well-established approach to take into account the effects of atmospheric turbulence in astronomical seeing. This is of key importance in designing adaptive optics for new-generation telescopes, in particular in view of applications such as exoplanet detection or long-exposure spectroscopy. We present an innovative approach to simulate turbulent phase that is based on stochastic realization theory. The method shows appealing properties in terms of both accuracy in reconstructing the structure function and compactness of the representation.