ABSTRACT
Most patients with gastric cancer are diagnosed at advanced clinical stages with a high frequency of lymph node metastasis. It is very important to find novel factors for the early diagnostic and prognostic evaluation of gastric cancer. It has been shown that IGF-1R activates mitotic division and inhibits apoptosis of cancer cells through the activation of signaling MAP/ERK and PI3K/Akt-1 pathways. IGF-1R plays a role in cell transformation and maintenance of the phenotype in modified cells. Moreover, an IGF-1 receptor effect influences the processes of adhesion, migration, invasion and metastasis of tumor cells. The aim of the study was to assess the expression of IGF-1R in gastric carcinoma in correlation with selected anatomo-clinical parameters. The study enrolled a group of 49 patients treated surgically for gastric cancer. 28 patients had no lymph node metastases. The expression of the studied proteins was assessed using the immunohistochemical method. We found that the expression of IGF-1R in gastric cancer is associated with lymph node metastasis (p < 0.001), is correlated with worse prognosis and high histological malignancy grade, and is an independent predictor of survival in patients with gastric cancer (p < 0.001). IGF-1R may play an important role in tumor growth and metastasis via the lymphatic pathway.
Subject(s)
Biomarkers, Tumor/analysis , Carcinoma/chemistry , Carcinoma/secondary , Receptor, IGF Type 1/analysis , Stomach Neoplasms/chemistry , Stomach Neoplasms/pathology , Aged , Biopsy , Carcinoma/mortality , Female , Humans , Immunohistochemistry , Lymphatic Metastasis , Male , Middle Aged , Neoplasm Grading , Stomach Neoplasms/mortality , Survival AnalysisABSTRACT
Tissue inhibitor of metalloproteinase-1 (TIMP-1) inhibits the ability of cancer cells to metastasize, but it can also stimulate cancer development. The aim of this study was to assess the level of TIMP-1 in serum and its expression in patients with colorectal cancer (CRC). The study group consisted of 43 patients diagnosed with colorectal cancer and 24 healthy volunteers. The level of TIMP-1 was assessed by the ELISA method while the expression of this protein was performed immunohistochemically. The concentration of TIMP-1 in the sera of colorectal cancer patients was significantly higher than in the healthy control group (p = 0.004). Higher level of TIMP-1 in the sera correlated with female gender (p = 0.045), tumor location in colon (p = 0.016), poorly differentiated tumor (p = 0.034) and higher platelet count in whole blood (p < 0.004). A positive reaction of the protein in cancer cells was observed in 31 cases and was found to correlate negatively with its reaction in peritumoral stroma (p < 0.001). According to this study, TIMP-1 protein may play an important role in cancer development. The assessment of this molecule in serum and tissue can be useful at the time of diagnosis and can help us to understand the nature of colorectal pathogenesis.
Subject(s)
Adenocarcinoma/blood , Biomarkers, Tumor/blood , Colorectal Neoplasms/blood , Tissue Inhibitor of Metalloproteinase-1/blood , Adult , Aged , Aged, 80 and over , Case-Control Studies , Female , Humans , Immunohistochemistry , Male , Middle AgedABSTRACT
PURPOSE: The aim was to compare preventive effect of total parenteral nutrition (TPN) and oral nutrition (preOp) on the perioperative insulin resistance prevention in surgical gastrointestinal cancer patients. MATERIAL/METHODS: The study was conducted in a group of 75 elective gastric and large intestine cancer patients. Patients were randomly divided into 3 study groups, 25 patients each: group I (NIL) - no preparations influencing tissue sensitivity to insulin, group II (TPN) - total parenteral nutrition in its preoperative stage and group III (TPN + preOp) parenteral nutrition and preOp in the preoperative phase. RESULTS: Immediately after the surgery, no statistically significant differences in insulin resistance level between groups were observed. During the first 6 postoperative hours, a statistically significant decrease of insulin resistance level in the TPN+ preOp group in comparison to others, was observed. During the first 24 postoperative hours, the NIL group was the only one to keep the insulin resistance level the same as in the preoperative phase. CONCLUSIONS: Application of TPN in the preoperative phase leads to shortening of perioperative insulin resistance time. Combining TPN with oral application of carbohydrate before surgical procedure is an effective and the best method in postoperative insulin resistance syndrome prevention.
Subject(s)
Carcinoma/complications , Carcinoma/surgery , Gastrointestinal Neoplasms/complications , Gastrointestinal Neoplasms/surgery , Insulin Resistance , Parenteral Nutrition, Total/methods , Adult , Aged , Aged, 80 and over , Carbohydrates/administration & dosage , Female , Humans , Insulin/metabolism , Male , Middle Aged , Postoperative Complications/prevention & control , Preoperative Period , Prospective Studies , Time FactorsABSTRACT
The aim of the study was to assess the expression of adhesion molecules, CD134 and CD137, in the peripheral blood in correlation with clinical advancement, the histological grade, the size and type of tumour growth, tissue infiltration, and lymph node and metastases to lymph nodes and liver. The study involved 28 patients with primary colorectal cancer. The expression of both molecules was investigated on the day of the surgery, before the procedure and ten days after the operation by means of flow cytometry. The expression of CD134 was markedly higher, compared to CD137, both on the day of the surgery and ten days after the operation. A significant increase was observed in CD134 expression ten days after the surgery. CD137 expression increased with the higher stage of clinical advancement, but decreased with the enhancement of colon wall infiltration. CD134 showed a similar expression for all the stages of tumour clinical advancement.
Subject(s)
Colorectal Neoplasms/pathology , Receptors, Nerve Growth Factor/blood , Receptors, Tumor Necrosis Factor/blood , Antigens, CD , Colorectal Neoplasms/blood , Colorectal Neoplasms/immunology , Colorectal Neoplasms/surgery , Flow Cytometry , Humans , Neoplasm Staging , Postoperative Period , Receptors, OX40 , Tumor Necrosis Factor Receptor Superfamily, Member 9ABSTRACT
The aim of this study was to evaluate the expression of CD44v10 in colorectal tumour cells and in lymphocytes infiltrating the tumour (CD45+). Samples of tumour tissue (TT), as well as of healthy tissue (HT) and of tumour adjacent tissue (TAT), were obtained from 20 patients. An evaluation of CD44v10 expression was performed in a flow cytometer. The mean value of the percentage of CD45+ with co-expression of CD44v10 was significantly higher in the lower stage of the tumour (pT). The mean value of the percentage of epithelial cells with CD44v10 co-expression was significantly higher in pN2 than in pN1 stage. Only in TAT the mean value of the percentage of epithelial cells and CD45+ with tCD44v10 co-expression was significantly lower in the higher degree of histological malignancy. It is supposed that CD44v10 takes part in local cancer progression.
Subject(s)
Colorectal Neoplasms/genetics , Genetic Variation , Hyaluronan Receptors/genetics , Lymphocytes, Tumor-Infiltrating/pathology , Colorectal Neoplasms/pathology , Humans , Lymphocytes, Tumor-Infiltrating/immunology , Neoplasm Recurrence, LocalABSTRACT
69 patients with rectal cancer were operated in years 1987-1996. Anastomosis was performed using stapler in 35 patients or hand suture in 34 patients. The authors analysed influence of the anastomosis technique and additional treatment on early and long-term postoperative results.
Subject(s)
Adenocarcinoma/therapy , Rectal Neoplasms/surgery , Adenocarcinoma/pathology , Adenocarcinoma/secondary , Adult , Aged , Anastomosis, Surgical , Chemotherapy, Adjuvant , Humans , Liver Neoplasms/secondary , Middle Aged , Neoplasm Staging , Radiotherapy, Adjuvant , Rectal Neoplasms/pathology , Suture Techniques , Treatment OutcomeABSTRACT
From September 1993 tol May 1997 360 laparoscopic cholecystectomies have been performed in patients aged 17-74: 318 women (88.3%) and 42 men (11.7%). 19 of them (5.3%) required a conversion to open surgery. In 13 cases (13.6%) conversion was caused by technical difficulties. In the other 6 (1.7%) was caused by intraoperative complications. While converted 15 simple cholecystectomies were performed, 2 with T-tube drainage of bile ducts, 1 with anastomosis choledochoduodenalis and 1 with partial stomach resection (Rydygier method). In all cases adhesions were divided. None of the patients died. Authors believe that LCh can be performed safely on most of the patients with chronic and acute cholecystitis.
Subject(s)
Cholecystectomy/methods , Cholelithiasis/surgery , Adolescent , Adult , Aged , Cholangiopancreatography, Endoscopic Retrograde , Cholecystectomy, Laparoscopic/adverse effects , Drainage/methods , Female , Humans , Male , Middle AgedABSTRACT
In the years 1972-1995 41 patients suffering from prolapse of the rectum were operated according to the Moore method. The method is based on the observations which suggest that the cause of prolapse is intestinal intussusception which can be prevented by translocating the anus under the pubic joint. The observation time is from 1 to 23 years. Two patients were reoperated; one-as a result of a recurrence of rectal prolapse and one due to a post-operative adhesive ileus. One patient died as result of peritonitis following an overlooked microperforation in the rectal wall. In the case of the remaining patients, the treatment was fully successful. Regular defecation cycle as well as incontination of stool and flatus returned and the anal sphincters almost fully regained their tension in comparison to the pre-operative state. Early and distant results of surgery of prolapse of the rectum are satisfactory.
