ABSTRACT
PURPOSE: This study evaluated the immunohistochemical presence of Indian Hedgehog (IHH), transforming growth factor-ß (TGF-ß), and parathyroid-1 receptor (PTH1R) in calvaria bone repair, and compared these results with the histological bone matrix features in defects treated with autograft in the presence or absence of L-PRP. MATERIAL AND METHODS: An artificial bone defect measuring 5 × 1 mm was produced in the calvaria of 28 Wistar rats. Randomly the defects were treated with autograft and autograft mixed with L-PRP. The animals were euthanized at 15 and 40 days post-surgery. Data were analyzed by Student-Newman-Keuls test (p ≤ .05) for immunohistochemical interpretation. RESULTS: The results revealed that the histological characteristic of bone matrix deposited in the defect was different in the defects treated with L-PRP. The group that received only the autograft demonstrated larger haversian bone matrix deposited, whereas the group that received autograft mixed with L-PRP revealed trabecular bone deposition. These results coincided with significantly higher immunopositivity for IHH, TGF-ß1, and PTH1R in the L-PRP group. CONCLUSION: These results suggest that L-PRP altered the biological characteristic of the autograft, increasing the bone cells IHH+ but inducing a trabecular bone associated with intense quantities of TGF-ß and PTH1R.
Subject(s)
Autografts/transplantation , Bone Matrix/physiology , Hedgehog Proteins/analysis , Leukocytes/physiology , Osteogenesis/physiology , Platelet-Rich Plasma/physiology , Receptor, Parathyroid Hormone, Type 1/analysis , Skull/surgery , Transforming Growth Factor beta1/analysis , Animals , Bone Diseases/surgery , Bone Matrix/pathology , Cancellous Bone/pathology , Cancellous Bone/physiology , Haversian System/pathology , Haversian System/physiology , Image Processing, Computer-Assisted/methods , Immunohistochemistry , Male , Photography/methods , Random Allocation , Rats , Rats, Wistar , Skull/pathology , Skull/physiologyABSTRACT
An adolescent boy with an Angle Class II Division 1 malocclusion had a hyperdivergent growth pattern with an excessive lower facial height and an anterior open bite. Combined orthodontic and surgical treatment should be considered for patients with a skeletal anterior open-bite malocclusion. For patients who do not want surgery, however, a 0.022 × 0.028-in nontorqued, nonangulated fixed appliance with a chincup as adjunct therapy is an alternative that can have excellent results. The final outcome of this treatment were great improvements in function, esthetics, and posttreatment stability after 8 years.
