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1.
Sci Rep ; 6: 39632, 2016 12 23.
Article in English | MEDLINE | ID: mdl-28008977

ABSTRACT

Tunneling Nanotubes (TNTs) are actin enriched filopodia-like protrusions that play a pivotal role in long-range intercellular communication. Different pathogens use TNT-like structures as "freeways" to propagate across cells. TNTs are also implicated in cancer and neurodegenerative diseases, making them promising therapeutic targets. Understanding the mechanism of their formation, and their relation with filopodia is of fundamental importance to uncover their physiological function, particularly since filopodia, differently from TNTs, are not able to mediate transfer of cargo between distant cells. Here we studied different regulatory complexes of actin, which play a role in the formation of both these structures. We demonstrate that the filopodia-promoting CDC42/IRSp53/VASP network negatively regulates TNT formation and impairs TNT-mediated intercellular vesicle transfer. Conversely, elevation of Eps8, an actin regulatory protein that inhibits the extension of filopodia in neurons, increases TNT formation. Notably, Eps8-mediated TNT induction requires Eps8 bundling but not its capping activity. Thus, despite their structural similarities, filopodia and TNTs form through distinct molecular mechanisms. Our results further suggest that a switch in the molecular composition in common actin regulatory complexes is critical in driving the formation of either type of membrane protrusion.


Subject(s)
Actin Cytoskeleton/metabolism , Actins/metabolism , Nanotubes/chemistry , Pseudopodia/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Animals , Brain/metabolism , Cell Adhesion Molecules/metabolism , Cell Communication , Cell Membrane/metabolism , Endocytosis , Green Fluorescent Proteins/metabolism , Mice , Microfilament Proteins/metabolism , Microscopy, Electron, Scanning , Nerve Tissue Proteins/metabolism , Neurons/metabolism , Phosphoproteins/metabolism , cdc42 GTP-Binding Protein/metabolism
3.
Elife ; 4: e09395, 2015 Nov 17.
Article in English | MEDLINE | ID: mdl-26575286

ABSTRACT

Synaptic target specificity, whereby neurons make distinct types of synapses with different target cells, is critical for brain function, yet the mechanisms driving it are poorly understood. In this study, we demonstrate Kirrel3 regulates target-specific synapse formation at hippocampal mossy fiber (MF) synapses, which connect dentate granule (DG) neurons to both CA3 and GABAergic neurons. Here, we show Kirrel3 is required for formation of MF filopodia; the structures that give rise to DG-GABA synapses and that regulate feed-forward inhibition of CA3 neurons. Consequently, loss of Kirrel3 robustly increases CA3 neuron activity in developing mice. Alterations in the Kirrel3 gene are repeatedly associated with intellectual disabilities, but the role of Kirrel3 at synapses remained largely unknown. Our findings demonstrate that subtle synaptic changes during development impact circuit function and provide the first insight toward understanding the cellular basis of Kirrel3-dependent neurodevelopmental disorders.


Subject(s)
Hippocampus/physiology , Membrane Proteins/metabolism , Mossy Fibers, Hippocampal/metabolism , Neurons/physiology , Synapses/metabolism , Animals , Cell Line , Gene Knockout Techniques , Hippocampus/embryology , Membrane Proteins/deficiency , Mice , Mice, Knockout , Rats
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