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1.
Ann Biol Clin (Paris) ; 62(1): 93-8, 2004.
Article in French | MEDLINE | ID: mdl-15047498

ABSTRACT

Luminex technology is a new flow cytometry technology enabling us to analyse numerous reactions in a unique tube or well. It is a multiplexed data acquisition and analysis platform of microsphere-based assays that performs simultaneous measurements of up to 100 different analytes. In the histo- compatibility field, individual sets of microspheres are modified with reactive components such as antigens in order to perform HLA antibodies identification, or with oligonucleotides in order to perform HLA typing after reverse PCR-SSO. Thus microspheres are the equivalent of a panel of HLA typed lymphocytes (for PRA determination and antibody identification) or equivalent to a large set of probes selected to assign HLA typing. This new tool can be very useful in HLA laboratories since it is very easy to use and the results are concordant with those obtained with reference technics.


Subject(s)
Flow Cytometry/methods , Histocompatibility Testing/methods , Polymerase Chain Reaction , Antibodies/isolation & purification , HLA Antigens/immunology , Microspheres
2.
Tissue Antigens ; 60(3): 268-70, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12445312

ABSTRACT

This communication reports the identification of a new HLA-DRB1*03 allele identified in three members of a Caucasian French family. This new allele has been officially named HLA-DRB1*0321 by the World Health Organization Nomenclature Committee. The complete exon 2 sequence of DRB1*0321 is identical to that of DRB1*0307 except for the first and second nucleotides of codon 37 (TT replacing AA), which lead to the substitution of a tyrosine for a phenylalanine (AAC-->TTC at position 37). The family study showed that this new allele was transmitted into the HLA-A*0101/09, -B*0801/14, -Cw*0701, -DRB1*0321, -DRB3*0101, -DQB1*0503 and -DPB1*0401 haplotype. The complete exon 2 sequence of this new allele has been previously deposited in the EMBL Sequence Database under accession number AF297266.


Subject(s)
Alleles , HLA-DR Antigens/genetics , Base Sequence , Bone Marrow Transplantation/immunology , Exons/genetics , Family Health , HLA-DRB1 Chains , Haplotypes , Humans , Molecular Sequence Data
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