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1.
Animals (Basel) ; 14(14)2024 Jul 10.
Article in English | MEDLINE | ID: mdl-39061494

ABSTRACT

BACKGROUND: Biosecurity measures are essential to improve animal health and welfare, tackle antimicrobial resistance (AMR), minimize the burden of infectious diseases, and enhance the safety, security, and quality of sustainable agricultural products. However, the implementation of these measures in small mountain farms can be influenced by several factors, which probably differ from the main variables that affect intensive farming systems. METHODS: This study investigated the awareness among farmers regarding the implementation of biosecurity measures at the local level in small dairy farms located in the Autonomous Province of Bolzano/Bozen (Italy). In order to determine to what extent these measures are in line with the recommendations, a questionnaire was conceptualized and sent by post to a representative group of local farmers. The initiative was voluntary and anonymous. RESULTS: A total of 50 farmers responded to the questionnaire, resulting in a response rate of 27.5%. This study confirms that most of the farmers are familiar with biosecurity, and 38% of them know its comprehensive definition. The results indicate that 77% of respondents are willing to implement preventive measures to reduce the use of antimicrobials, and 76% of them acknowledge that they would follow the recommendations provided by veterinarians. In agreement with other studies, the role of the veterinarian as a person of trust among farmers is confirmed. CONCLUSIONS: Main strengths and areas of improvement have been identified. Additional data and effective tools are needed to better investigate drivers toward biosecurity and to implement impactful and practical measures for mountain farming.

2.
PLoS One ; 16(12): e0252972, 2021.
Article in English | MEDLINE | ID: mdl-34972117

ABSTRACT

SARS-CoV-2 has clearly shown that efficient management of infectious diseases requires a top-down approach which must be complemented with a bottom-up response to be effective. Here we investigate a novel approach to surveillance for transboundary animal diseases using African Swine (ASF) fever as a model. We collected data both at a population level and at the local level on information-seeking behavior respectively through digital data and targeted questionnaire-based surveys to relevant stakeholders such as pig farmers and veterinary authorities. Our study shows how information-seeking behavior and resulting public attention during an epidemic, can be identified through novel data streams from digital platforms such as Wikipedia. Leveraging attention in a critical moment can be key to providing the correct information at the right moment, especially to an interested cohort of people. We also bring evidence on how field surveys aimed at local workers and veterinary authorities remain a crucial tool to assess more in-depth preparedness and awareness among front-line actors. We conclude that these two tools should be used in combination to maximize the outcome of surveillance and prevention activities for selected transboundary animal diseases such as ASF.


Subject(s)
African Swine Fever/epidemiology , Epidemics/prevention & control , Epidemiological Monitoring , Livestock/virology , Animals , Awareness , Estonia/epidemiology , Farmers , Internet , Statistics, Nonparametric , Surveys and Questionnaires , Swine
3.
Vet Parasitol Reg Stud Reports ; 21: 100432, 2020 07.
Article in English | MEDLINE | ID: mdl-32862903

ABSTRACT

The Autonomous Province of Bolzano-South Tyrol (APB), located in the northernmost territory of the Italian eastern Alps, is still considered non-endemic for canine leishmaniosis (CanL) despite clinical cases being observed and a competent Leishmania infantum vector (Phlebotomus perniciosus) having been recorded since 2008. A serological survey of leishmaniosis among a randomly-selected subpopulation of registered owned dogs was carried in 2018, followed by entomological investigations performed in 2019 and driven by canine survey results. A total of 457 resident dogs from all over the APB territory were examined through IFAT for antibodies against L.infantum, of which 63 (13.8%) tested positive. Thirty-five seropositive cases (7.7%) were considered autochthonous to APB, i.e. dogs born and lived in the province, or imported dogs with no travel history in the past 5 years. Most of these animals showed an antibody titre at the threshold level of 1:40, suggesting a low degree of parasite transmission/contacts. In 2 autochthonous cases with moderately high IFAT titre, the infection was confirmed by nested-PCR in peripheral blood. Thirty-one georeferenced sites were monitored for sand flies by means of interception (sticky papers) and attraction (CDC miniature light traps) collection devices. Traps were set during summer approximately on monthly basis, and extended up to October for positive sites. Only 2 sites were found positive for a total of 317 phlebotomine specimens collected by sticky traps, which included a previously known P. perniciosus-endemic site near Bolzano town. Sergentomyia minuta was by far the most prevalent (98.1%) and the only recorded sand fly species in the most northerly Italian site ever investigated (Coldrano municipality in Venosta valley). For the first time, Leishmania serology and n-PCR positive dogs autochthonous to APB were identified, however the spread of sand flies competent for L. infantum transmission could not be demonstrated in several places where endemic seropositive cases were recorded. APB can be considered a territory of low CanL endemicity, however awareness and continuous monitoring are needed to detect changes in the epidemiological status of the zoonosis.


Subject(s)
Animal Distribution , Arthropod Vectors/physiology , Dog Diseases/epidemiology , Leishmania infantum/isolation & purification , Leishmaniasis, Visceral/veterinary , Phlebotomus/physiology , Animals , Dog Diseases/parasitology , Dogs , Female , Italy/epidemiology , Leishmaniasis, Visceral/epidemiology , Leishmaniasis, Visceral/parasitology , Male , Polymerase Chain Reaction/veterinary , Prevalence , Seroepidemiologic Studies
4.
J Mater Sci Mater Med ; 23(9): 2151-62, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22706626

ABSTRACT

A cochlear implant (CI)-associated local drug delivery system based on dexamethasone (DMS) was developed with the purpose to inhibit the growth of fibrotic tissue which influences the signal transmission from the CI to the neurons of the inner ear. For the realization of a targeted DMS delivery the following concepts were combined: modification of the silicone-based electrode carrier by incorporation of DMS and a DMS-containing polymeric coating chemically attached on the surface of the electrode carrier. It was demonstrated that the coated CI showed a high coating stability in a simulated implantation procedure. The in vitro drug release studies in a quasi-stationary model revealed a faster DMS release in the initial phase originating from the DMS-containing coatings and then a lower and sustained DMS release originating from the DMS-loaded silicone carrier. The performed in vitro biocompatibility study confirmed that the released DMS was non-toxic for cultured spiral ganglion cells.


Subject(s)
Cochlear Implantation/adverse effects , Cochlear Implants , Drug Delivery Systems , Ear, Inner/pathology , Postoperative Complications/prevention & control , Animals , Animals, Newborn , Cell Survival/drug effects , Cells, Cultured , Coated Materials, Biocompatible/chemical synthesis , Coated Materials, Biocompatible/chemistry , Cochlear Implantation/methods , Cochlear Implants/adverse effects , Fibrosis/prevention & control , Injections, Intralesional , Materials Testing , Organ Specificity , Oxygen/administration & dosage , Rats , Rats, Sprague-Dawley , Silicones/administration & dosage , Silicones/pharmacology , Spiral Ganglion/cytology , Spiral Ganglion/drug effects , Spiral Ganglion/pathology
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