ABSTRACT
Black carrots contain anthocyanins possessing enhanced physiological activities. Explants of young black carrot shoots were cultured in Murashige and Skoog (MS) medium for callus initiation and were transferred to new MS medium supplemented with four different combinations of 2,4-dichlorophenoxyacetic acid and kinetin. Subsequently, the lyophilized calli and black carrot harvested from fields were subjected to ultrasound extraction with ethanol at a ratio of 1:15 (w:v). Obtained extracts were applied to various human cancer cell lines including MCF-7 SK-BR-3 and MDA-MB-231 (human breast adenocarcinomas), HT-29 (human colon adenocarcinoma), PC-3 (human prostate adenocarcinoma), Neuro 2A (Musmusculus neuroblastoma) cancer cell lines and VERO (African green monkey kidney) normal cell line by MTT assay. The highest cytotoxic activity was achieved against Neuro-2A cell lines exhibiting viability of 38-46% at 6.25 µg/ml concentration for all calli and natural extracts. However, a significantly high IC50 value of 170.13 µg/ml was attained in normal cell line VERO indicating that its natural counterpart is an ideal candidate for treatment of brain cancer without causing negative effects to normal healthy cells.
Subject(s)
Antineoplastic Agents, Phytogenic/therapeutic use , Daucus carota/chemistry , Neoplasms/drug therapy , Plant Extracts/therapeutic use , 2,4-Dichlorophenoxyacetic Acid/administration & dosage , Animals , Anthocyanins/analysis , Breast Neoplasms , Cell Line, Tumor , Chlorocebus aethiops , Culture Media , Daucus carota/growth & development , Female , HT29 Cells , Humans , Kinetin/administration & dosage , MCF-7 Cells , Male , Mice , Neuroblastoma , Prostatic Neoplasms , Tissue Culture Techniques , Vero CellsABSTRACT
In this study, ethanol, methanol, ethyl acetate and water extracts of Rubia tinctorum L. (Rubiaceae) were tested for antimicrobial activity by the disc diffusion method. From the present study it was found that Rubia tinctorum L. revealed antimicrobial activity against some Gram (+) and Gram (-) bacteria, yeasts, filamentous fungi and actinomycetes.
