Monthly microplastics change in European anchovy's (Engraulis encrasicolus) gastrointestinal tract in the Black Sea.

To contribute to a better understanding of the regional dynamics of MP pollution and its potential effects on the anchovy population and human health, here we assessed the presence and characterization of microplastics (MPs) in European anchovy (Engraulis encrasicolus), which is the most caught/consumed species in the Black Sea and is of vital importance to the ecosystem. A total of 360 individuals (30 per month) were sampled from the eastern Black Sea continental shelf all year round (monthly from September 20 to Aug 21). We extracted and digested the gastrointestinal tracts (GITs) of the samples with H2O2 and characterized the MPs in the GITs by stereomicroscope and ATR-FTIR. MPs varied between 0 and 0.43 MP individual-1. The morphological structure of MPs was composed of 51 % fiber > fragment (32.7 %) > film (12.2 %) > foam (4.1 %) and polymer types as PP (42.9 %) and PE (22.4 %). In the prevalence of MP colors, black (26.5 %) > white (24.5 %) > red (22.5 %) was observed. The mean MP size was 735.32 ± 836.62 µm, with no significant correlation between the abundance and size of ingested MPs and anchovy height/weight and GIT weight. We determined that MP abundance showed substantial differences between the fishing season (0.18 ± 0.05 MP ind-1) and the banned season (0.05 ± 0.03 MP ind-1). It is essential to develop effective waste management strategies to protect the vulnerable marine ecosystems of the Black Sea and ensure sustainable exploitation of living resources in this region. These strategies should be accompanied by robust monitoring and enforcement measures to guarantee their effectiveness and compliance.

Accumulation of phenolic compounds and expression of phenylpropanoid biosynthesis-related genes in leaves of basil transformed with A. rhizogenes strains.

Biotic factors affect the content of secondary metabolites by interfering with molecular and biochemical pathways. In the current study, A. rhizogenes strains were inoculated into basil (Ocimum basilicum) to examine the effect of plant-microbe interaction on the accumulation of monomeric phenolic metabolites and the transcript levels of selected genes involved in the biochemical synthesis of secondary compounds. Initially, the integration of the rolB gene was validated by performing PCR analysis on genomic DNA samples from the basil plant inoculated with A. rhizogenes strains. We have detected that the accumulation of mRNA transcripts linked to the biosynthesis pathway of phenolic compounds has higher transcript expression levels in the leaves of transformed basil in proportion to uninoculated plants. Basil plants inoculated with A. rhizogenes 39207 strain had higher transcript levels of CAD, C4H, TAT, FLS, EGS, HPPR, PAL, and RAS genes than other experimental groups. We have identified eleven phenolic components, and the level of rosmarinic acid, eugenol, chicoric acid, and rutin increased in the inoculated basil leaves. However, the inoculation of A. rhizogenes did not cause a change in the compounds of chlorogenic acid, methyl chavicol, cinnamic acid, quercetin, vanillic acid, and caffeic acid. In conclusion, the increase in basic secondary metabolites could be achieved by the A. rhizogenes-mediated transformation of basil plants, and especially ATCC 43057 strain may be one of the A. rhizogenes strains. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01320-w.

HSF and Hsp Gene Families in sunflower: a comprehensive genome-wide determination survey and expression patterns under abiotic stress conditions.

Sunflowers belong to the Asteraceae family, which comprises nutrimental and economic oilseed plants. Heat shock proteins (Hsps) are protein families vital for all organisms' growth and survival. Besides the ordinary conditions, the expression of these proteins ascends during abiotic stress factors such as high temperature, salinity, and drought. Using bioinformatics approaches, the current study identified and analyzed HSF and Hsp gene family members in the sunflower (Helianthus annuus L.) plant. HSF, sHsp, Hsp40, Hsp60, Hsp70, Hsp90, and Hsp100 domains were analyzed in the sunflower genome, and 88, 72, 192, 52, 85, 49, and 148 genes were identified, respectively. The motif structures of the proteins in the same phylogenetic tree were similar, and the α-helical form was dominant in all the protein families except for sHsp. The estimated three-dimensional structure of 28 sHsp proteins was determined as ß-sheets. Considering protein-protein interactions, the Hsp60-09 protein (38 interactions) was found to be the most interacting protein. The most orthologous gene pairs (58 genes) were identified between Hsp70 genes and Arabidopsis genes. The expression analysis of selected genes was performed under high temperature, drought, and high temperature-drought combined stress conditions in two sunflower cultivars. In stress conditions, gene expressions were upregulated for almost all genes in the first half and first hours at large. The expressions of HanHSF-45 and HanHsp70-29 genes were raised in two cultivars under high temperature and high temperature-drought combined stress conditions. This study presents a blueprint for subsequent research and delivers comprehensive knowledge of this vital protein domain.

Potential habitats of an alien species (Asterias rubens Linnaeus, 1758) in the Black Sea: its current and future distribution patterns.

The Atlantic common starfish, Asterias rubens, has arrived firstly at the Marmara Sea in 1996 and to the Black Sea in 2007. In this study, we have exhibited the possible potential distribution of Asterias rubens throughout the Black Sea. For this, we predicted and determined the present and future distributions, and habitat preferences of this starfish in the Black Sea using environmental variables. The ecological niche modeling was used to detect the suitable habitat of A. rubens. In the current model, shallow areas seem to be the suitable habitat for A. rubens. However, this trend may change in the future distribution pattern. For the future projection, two representative concentration pathways (RCPs) that are a greenhouse gas concentration was used: RCP2.6 that is likely to keep global temperature rise below 2 °C by 2100 and RCP8.5 that will happen approximately 5 °C in range of global mean temperature increase in 2100 from pre-industrial baseline. According to RCP2.6 scenarios as well as the RCP8.5 scenario in 2040-2050, the suitable habitats in the Black Sea will probably decrease due to climate change. The most suitable habitats in these scenarios will remain the western and southern coasts of the Black Sea because these areas will be less affected by the change in the climate. In contrast, for the 2090-2100 periods of the RCP8.5, there will likely be a significant unsuitable habitat throughout the Black Sea. Therefore, the suitable habitat for A. rubens will be restricted to the western and southern coasts of the Black Sea.

Identification of radical structures on 1-pentamethylbenzyl-3-ethylimidazoliumsilver(I)bromide and 1,3-bis(pentamethylbenzyl)-4,5-dimethylbenzimidazoliumsilver(I)bromide exposed to gamma rays: an EPR study.

1-Pentamethylbenzyl-3-ethylimidazoliumsilver(I)bromide and 1,3-bis(pentamethylbenzyl)-4,5dimethylbenzimidazoliumsilver(I)bromide and their Ag+ complexes were synthesized and their polycrystal forms were produced by recrystallization in dichloromethane/Et2 O solvent system. Structural determinations were carried out by 1 H NMR and 13 C NMR with a Varian 400 NMR system using tetramethylsilane as internal standard and CDCl3 as solvent. The disappearance of acidic N-heterocyclic carbene proton showed the formation of Ag(I) complexes. Also, elemental analyses were carried out. Electron paramagnetic resonance (EPR) measurements were performed to determine the formed radical structure on the samples irradiated at the room temperature for 72 h by using 60 Co-source with dose rate of 0.680 kGy. The EPR measurements were carried out in the temperature range of 200 K-450 K. Identical radicals were determined on the irradiated compounds. It was observed that the shapes of the spectra of the samples were independent of the temperature but, the resonance line intensities changed linearly with the temperature. Also, it was detected that the free radical on the 1-pentamethylbenzyl-3-ethylimidazoliumsilver(I)bromide is not stable compared to that on the 1,3-bis(pentamethylbenzyl)-4,5dimethylbenzimidazoliumsilver(I)bromide. Copyright © 2016 John Wiley & Sons, Ltd.