[The anticoagulant action of a factor from the nondialyzed fraction of the ammoniacal extract of the lungwort, Pulmonaria mollissima]. / Protivosvertyvaiushchee deistvie antikoagulianta nedializuemoi fraktsii ammiachnogo ékstrakta iz travy medunitsy miagchaishei.

It has been established that a non-dialyzed fraction of ammoniacal extract from P. molissima contains an anticoagulant-glycopeptide. In in vivo experiments, it produces stable hypocoagulemia, primarily blocking fibrin self-assemblage, and prevents the animal's death from thromboplastinemia. It has been found that the aforesaid action does not exert any adverse effect on the blood contents, arterial pressure, respiration or urinary renal function.

[Anticoagulants of the nondialyzed fractions of the ammonia extract of the herb Pulmonaria mollissima]. / Antikoagulianty nedializuiushchei fraktsii ammiachnogo ékstrakta travy medunitsy miagchaishei.

T-100 anticoagulants non-dialyzed through cellophane are isolated from ammonia extract of herb Pulmonaria mollissima. Their effect is mainly realized at the stage of coagulation conversions of fibrinogen, first of all at the stage of fibrin self-assembly. One of these anticoagulants is a peptide, another one is a glycopeptide which contains glucose residues like carbohydrate. Peptide components of the both anticoagulants are characterized by the high level of amino acids, their radicals are capable to ionization under physiological conditions. In contrast to animal-origin analogs, glycopeptide in nontoxic doses causes stable hypocoagulemia in animals. It is expedient to study Pulmonaria mollissima extracts as a source of direct anticoagulants.

[A comparative study of the efficacy of praziquantel and its liposomal form in experimental opisthorchiasis]. / Sravnitel'noe izuchenie éffektivnosti prazikvantelia i ego liposomal'noi formy pri éksperimental'nom opistorkhoze.

Intensive and extensive efficacy of praziquantel pure and that included in multilamellar liposomes was studied. As a result, a high anti-helminthic activity of liposomal praziquantel has been established. Negative effect of praziquantel on protein synthetic and enzymatic function of the liver has been revealed in normal animals. The treatment with liposomal praziquantel led to a rapid normalization of total lipid and bilirubin levels, thymol turbidity test, transaminase indices and also to a decrease in dystrophic liver changes.

[Effect of tri-iodine-containing x-ray contrast substances on the blood coagulation system in an experiment]. / Vliianie nekotorykh triiodsoderzhashchikh rentgenokontrastnykh veshchestv na sistemu svertyvaniia krovi v éksperimente.

Experiments on white rats were made to examine the time course of hemocoagulation changes induced by intravenous injection of the triiodine-containing radiographic contrast agents, bilignost, verografin, triombrast, iodamide-380. The substances were found to exert a biphasic action: an initial decrease in the total coagulation is replaced by a tendency toward hypercoagulemia. In experiments in vitro, the radiographic contrast agents increased the time of plasma recalcification, the prothrombin time and retarded the thrombin-fibrinogen and thrombin-plasma reactions. Bilignost produced the most marked hypocoagulemia. It is suggested that the direct effects of the drugs on blood coagulation involve a decrease in the total blood coagulation activity.

[Anticoagulant effect of phosphatidylserine]. / Protivosvertyvaiushchii éffekt fosfatidilserina.

Intravenous administration of phosphatidyl serine emulsion (30 and 60 mg/kg of body mass) was accompanied by an immediate decrease in the coagulation activity of blood, intensity and duration of which were dose-dependent. Phosphatidyl serine inhibited formation of thrombin in the prothrombin complex, activated by tissue thromboplastin or factor Xa in presence of Ca2+ in vitro. Kinetics of inhibition was similar in both cases, thus suggesting the interrelationship of phosphatidyl serine with prothrombin. Phosphatidyl serine inhibited also the effect of factor Xa on synthetic substrate BAME, interacting with the latter; these data were corroborated by study of absorbance spectra of phosphatidyl serine, BAME and their mixture. Thus, phosphatidyl serine inhibited thrombinogenesis due to a decrease of the interaction rate between factors Xa and II. Effect of phosphatidyl serine on activation of factor X required a special study. Phosphatidyl serine appears to participate in blood stabilization.

[Mechanism of the hypocoagulemic effect and the active principle of a phosphatidylserine-containing anticoagulant]. / Mekhanizm gipokoagulemicheskogo éffekta i deistvuiushchee nachalo fosfatidilserinsoderzhashchego antikoagulianta.

Experiments on animals in vitro showed that phosphatidylserine-containing anticoagulant (PhCA) inhibited factors X and VIII, restricted factor V activity and, to a less degree, that of factor VII, decreased the activity of fibrin-stabilizing factor, the degree of clot retraction and its tolerance to fibrinolysin. The activity of factor IX remained virtually unchanged under the influence of PhCA, whereas the contact phase was speeded up by 14%. Anticoagulant activity of PhCA was accounted for by the phosphatidyl molecule residue - glycerylphosphorylserine.

[Effect of an intravenously administered phosphatidylserine emulsion on blood coagulation and blood system indices]. / Vliianie vnutrivenno vvedennoi émul'sii fosfatidilserina na gemokoaguliatsiiu i nekotorye pokazateli sistemy krovi.

Experiments on white rats and rabbits showed that intravenous injection of phosphatidylethanol amine- and sphyngomyelin-stabilized phosphatidylserin emulsion produced hypocoagulemia. The effect was observed immediately after injection of the drug, reached a maximum after one hour and returned to normal following 24 hours. The changes became more intensive as the dose was increased and could be reproduced during repeated administrations over a period of ten days. The arterial blood pressure, velocity of the blood flow and volume of the circulating blood remained unchanged. There were no appreciable changes in the peripheral blood parameters or in bone marrow hemopoiesis on repeated administrations over a period of thirty days.