Subject(s)
Gastritis/drug therapy , Gastrointestinal Agents/therapeutic use , Adolescent , Adsorption , Adult , Bile Reflux/complications , Female , Humans , Male , Middle Aged , PolystyrenesSubject(s)
Bacteriophages , Bronchial Diseases/therapy , Enterobacteriaceae Infections/therapy , Pseudomonas Infections/therapy , Rhinitis, Allergic, Perennial/therapy , Adult , Aged , Asthma/therapy , Bronchiectasis/therapy , Bronchitis/therapy , Chronic Disease , Female , Humans , Male , Middle AgedSubject(s)
Asthma/etiology , Asthma/diagnosis , Asthma/physiopathology , Bronchi/physiopathology , HumansSubject(s)
Lung Diseases, Obstructive/diagnosis , Adolescent , Adult , Aged , Carbon Dioxide/blood , Female , Humans , Lung/diagnostic imaging , Male , Middle Aged , Oxygen/blood , Radiography , SpirometryABSTRACT
The present studies deal with histamine binding by some serum proteins of persons with allergic disorders and of normal subjects. It was found, that a defect in histamine inactivation occurs in patients with infectious bronchial asthma. There was no impairment of histaminopexy in the cases of hay fever and Quincke's edema.
Subject(s)
Carrier Proteins/metabolism , Histamine/metabolism , Hypersensitivity/metabolism , Adolescent , Adult , Aged , Blood Proteins/metabolism , Female , Humans , Male , Middle AgedABSTRACT
Human serum proteins were bound on and eluted from sepharose-polylysine-histamine column. Thus obtained protein fraction was referred to as HBP (Histamine Binding Protein). HBP proteins were examined for the ability to bind histamine by biologic method applied to isolated guinea pig intestine. Chromatographic separations were run on DEAE column to obtain HBP proteins which are directly responsible for histamine binding activity. The results obtained suggest that human serum contains 3 histamine binding fractions. One of them was identified as orosomucoid, and the 2 remaining ones seem to be glycoproteids belonging to alpha 1 globulin group.
Subject(s)
Blood Proteins/isolation & purification , Histamine/metabolism , Receptors, Histamine/isolation & purification , Chromatography, DEAE-Cellulose , Electrophoresis, Polyacrylamide Gel , HumansABSTRACT
The sorption of bile acids, bilirubin, bilirubin glucuronates and cholesterol on ion-exchange resin is described. After some successful attempts of sorption of these substances from human and animal bile in vitro, this exchanger has been used in human therapy. Positive results obtained showed the usefulness of the method. The purpose of the experiments was to study the usefulness of ion-exchangers for sorption of bile components of bile acids, bilirubin, bilirubin glucuronates and cholesterol. First sorption was carried out on human and animal bile, on Zerolit-FS-ip, in cycles (OH-) and (Cl-), by passing through glass columns downwards and some of them by mixing the bile with ion-exchange resin. Zerolit FS-ip is an organic sorbent, a scavenger, a strongly basic macroporous, anion exchanger. Positive results of these sorptions indicated the possibility of using this method in therapy of disturbed lipid conditions.
