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1.
Int J Cancer ; 136(10): 2284-92, 2015 May 15.
Article in English | MEDLINE | ID: mdl-25346337

ABSTRACT

The spontaneous metastasis from human gastric carcinoma (GC) remains poorly reproduced in animal models. Here, we established an experimental mouse model in which GC progressively developed in the orthotopic stomach wall and metastasized to multiple organs; the tumors colonized in the ovary exhibited typical characteristics of Krukenberg tumor. The expression of mesenchymal markers was low in primary tumors and high in those in intravasating and extravasating veins. However, the expression of epithelial markers did not differ, indicating that the acquisition of mesenchymal markers without a concordant loss of typical epithelial markers was associated with metastasis. We identified 35 differentially expressed genes (DEGs) in GC cells metastasized to ovary, among which overexpression of GAGE12 family genes, the top-ranked DEGs, were validated. In addition, knockdown of the GAGE12 gene family affected transcription of many of the aforementioned 35 DEGs and inhibited trans-well migration, tumor sphere formation in vitro and tumor growth in vivo. In accordance, GAGE12 overexpression augmented migration, tumor sphere formation and sustained in vivo tumor growth. Taken together, the GAGE12 gene family promotes GC growth and metastasis by modulating the expression of GC metastasis-related genes.


Subject(s)
Antigens, Neoplasm/genetics , Antigens, Neoplasm/metabolism , Neoplasm Metastasis/genetics , Stomach Neoplasms/pathology , Animals , Cell Line, Tumor , Epithelial-Mesenchymal Transition , Female , Gene Expression Regulation, Neoplastic , Humans , Mice , Mice, Nude , Neoplasm Proteins/genetics , Neoplasm Proteins/metabolism , Neoplasm Transplantation , Ovarian Neoplasms/genetics , Ovarian Neoplasms/metabolism , Ovarian Neoplasms/secondary , Signal Transduction , Stomach Neoplasms/genetics , Stomach Neoplasms/metabolism
2.
Mol Cells ; 32(6): 555-60, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22083306

ABSTRACT

Downregulation of the CD99 antigen on the surface of Hodgkin's lymphoma (HL) cells via EBV LMP1-mediated NF-κB suppression of Sp1 transcriptional activity is known to be associated with the appearance of pathogenic Reed-Sternberg cells. Here, we show that in addition, EBV LMP1 heterologous NF-κB activators such as CD30 and CD40 repress the CD99 promoter, which contains multiple Sp1-binding sites but no NF-κB binding sites. In addition, NF-κB-inducing kinase (NIK) repressed the CD99 promoter while NIK kinase mutants and JNK inhibitory protein failed to do so. Of the NF-κB subunits, NF-κB2 (p52) alone or in combination with other Rel subunits consistently inhibited the CD99, while NF-κB1 (p50) showed a marginal repressive effect. Furthermore, while transfection of LMP1 repressed the CD99 promoter in wild-type or NF-κB1 deficient MEFs, the same repression was not observed in NF-κB2 (p52)-deficient MEFs, indicating that NF-κB2 (p52) is required for LMP1-mediated repression of the CD99 promoter. Consistently, basal activity of the CD99 promoter was significantly higher in IKKα(-/-) and IKKß(-/-) MEFs, but not in IKKΓ(-/-) MEFs compared to the wild-type control MEFs. Sp1-binding sites were directly used in the repression, because a synthetic Sp1 reporter with 10 Sp1-binding sites from the CD99 promoter was repressed by LMP1 or p52 transfection. These data indicate that LMP1-mediated NF-κB2 exhibits the major inhibitory role in the transcription at the CD99 promoter.


Subject(s)
Antigens, CD/genetics , Cell Adhesion Molecules/genetics , NF-kappa B p52 Subunit/physiology , Promoter Regions, Genetic , Sp1 Transcription Factor/metabolism , Transcription, Genetic , 12E7 Antigen , Animals , Antigens, CD/metabolism , CD40 Antigens/biosynthesis , Cell Adhesion Molecules/metabolism , Gene Expression Regulation , Genes, Reporter , HEK293 Cells , Humans , JNK Mitogen-Activated Protein Kinases/metabolism , Ki-1 Antigen/biosynthesis , Luciferases/biosynthesis , Luciferases/genetics , Mice , NF-kappa B p52 Subunit/metabolism , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Recombinant Fusion Proteins/biosynthesis , Viral Matrix Proteins/biosynthesis , NF-kappaB-Inducing Kinase
3.
Biochem Biophys Res Commun ; 372(2): 298-304, 2008 Jul 25.
Article in English | MEDLINE | ID: mdl-18501190

ABSTRACT

Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid cohesion during S phase. Here, we report that Esco2 interacts with several component proteins of the CoREST complex, including a transcription corepressor CoREST, histone demethlyase LSD1, HDAC1, HDAC2, BRAF35, and PHF21A. Esco2 also interacts with various histone methyltransferases Suv39h1, SETDB1 and G9a. Esco2 complex purified from HeLa nuclear extract possesses histone H3 K9 methylation activity and functions as a transcription repressor. Esco2 fused to Gal4 DNA binding domain represses transcription by increasing methylation of histone H3 K9 in the promoter region. These results suggest a novel function of Esco2 in transcription repression through modulation of the chromatin structure.


Subject(s)
Acetyltransferases/metabolism , Chromatin/enzymology , Chromosomal Proteins, Non-Histone/metabolism , Histone-Lysine N-Methyltransferase/metabolism , Repressor Proteins/metabolism , Transcription, Genetic , Acetyltransferases/genetics , Cell Line , Chromosomal Proteins, Non-Histone/genetics , HeLa Cells , Histone Methyltransferases , Histone-Lysine N-Methyltransferase/genetics , Histones/metabolism , Humans , Methyltransferases/metabolism , Protein Methyltransferases/metabolism , Repressor Proteins/genetics
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