The cadmium-induced changes in the polar and neutral lipid compositions suggest the involvement of triacylglycerol in the defense response in maize.

Cadmium (Cd) is a heavy metal ion leading to morphological and physiological disorders in plants; a specific toxicity target is the membrane lipids. The total lipids were separated by thin-layer chromatography, and the fatty acid composition of the total (TLs), polar lipids (PLs) and triacylglycerol (TAG)-a neutral lipid-was analyzed in maize seedlings in hydroponics and treated by various Cd concentrations (0-200 µM Cd). The TLs and PLs significantly decreased in roots after Cd treatment, suggesting the onset of lipid peroxidation mediated by oxygen free radicals, that induce alterations of the membrane structure and function. There were also increases in the TAG from 28.2 to 36.9% of TLs, and the TAG/PLs ratio varied from 0.59 to 0.84, in control and after exposure to 200 µM Cd, respectively. The TAG plays potent roles in membrane turnover serving as energy and carbon resources for the biosynthesis of membrane lipids, to preserve membrane structure and function, and therefore cell homeostasis in response to Cd. In shoots, a significant increase in the levels of C16:0, C18:1, and C18:2, while a decrease in that of C18:3 was observed, suggesting inhibition of desaturases enzymes. These lead to impairment of the chloroplast membrane. The total lipid content did not change under Cd stress. The PLs, however, decreased from 22.4 to 13.6 mg g-1 DW; their percent to TLs varied from 86.6 to 52.5%, in control, and after Cd treatment, respectively. In conclusion, the accumulation of TAG may represent a defense strategy by which maize seedlings can withstand the effects of Cd toxicity, leading to reduced oxidative stress.

Joint genetic and network analyses identify loci associated with root growth under NaCl stress in Arabidopsis thaliana.

Plants have evolved a series of tolerance mechanisms to saline stress, which perturbs physiological processes throughout the plant. To identify genetic mechanisms associated with salinity tolerance, we performed linkage analysis and genome-wide association study (GWAS) on maintenance of root growth of Arabidopsis thaliana in hydroponic culture with weak and severe NaCl toxicity. The top 200 single-nucleotide polymorphisms (SNPs) determined by GWAS could cumulatively explain approximately 70% of the variation observed at each stress level. The most significant SNPs were linked to the genes of ATP-binding cassette B10 and vacuolar proton ATPase A2. Several known salinity tolerance genes such as potassium channel KAT1 and calcium sensor SOS3 were also linked to SNPs in the top 200. In parallel, we constructed a gene co-expression network to independently verify that particular groups of genes work together to a common purpose. We identify molecular mechanisms to confer salt tolerance from both predictable and novel physiological sources and validate the utility of combined genetic and network analysis. Additionally, our study indicates that the genetic architecture of salt tolerance is responsive to the severity of stress. These gene datasets are a significant information resource for a following exploration of gene function.

Aluminum mediates compositional alterations of polar lipid classes in maize seedlings.

Changes in lipid composition were investigated on maize roots and shoots under aluminum stress. After 4d exposure to 100 microM Al, root growth was inhibited while shoot growth was not affected. In roots, the decrease of the DBI (double bond index) of total fatty acids may signal a decrease in membrane fluidity. The total lipids (TL) decreased by 49%, but phospholipids (PL), phosphatidylcholine (PC) and phosphatidylinositol (PI) increased to approximately 3-fold. The MGDG increased to 2-fold but no significant change was found in the DGDG. The steryl lipids (SL) increased by 69%. The SL/PL ratio decreased from 2.64 to 1.52 and the MGDG/DGDG ratio increased from 0.45 to 1.06 in roots of Al-stressed plants. Al leads to oxidative stress in roots of treated plants as indicated by the increase of malondialdehyde (MDA) concentrations. In shoots, changes in fatty acid composition were associated with an increase of the DBI in all lipid classes except that of the DGDG decreased. The PG was the lipid class which shows the large variation of fatty acid composition. No significant changes were found either for TL, PL, SL or MDA concentrations in shoots of Al-treated plants. While PE levels did not show significant change, PI and PG increased and PC decreased. However, the Al caused 87% decrease in the GL levels. The MGDG and DGDG decreased to 19- and 8-fold, respectively. The deleterious effects of Al on polar lipids could be caused by a direct intervention of Al on plasma membrane and/or alteration of cell metabolism.