Blue light regulated lignin and cellulose content of soybean petioles and stems under low light intensity.

To improve light harvest and plant structural support under low light intensity, it is useful to investigate the effects of different ratios of blue light on petiole and stem growth. Two true leaves of soybean seedlings were exposed to a total light intensity of 200µmolm-2 s-1 , presented as either white light or three levels of blue light (40µmolm-2 s-1 , 67µmolm-2 s-1 and 100µmolm-2 s-1 ) for 15days. Soybean petioles under the low blue light treatment upregulated expression of genes relating to lignin metabolism, enhancing lignin content compared with the white light treatment. The low blue light treatment had high petiole length, increased plant height and improved petiole strength arising from high lignin content, thus significantly increasing leaf dry weight relative to the white light treatment. Compared with white light, the treatment with the highest blue light ratio reduced plant height and enhanced plant support through increased cellulose and hemicellulose content in the stem. Under low light intensity, 20% blue light enhanced petiole length and strength to improve photosynthate biomass; whereas 50% blue light lowered plants' centre of gravity, preventing lodging and conserving carbohydrate allocation.

Performance of targeted next-generation sequencing in the detection of respiratory pathogens and antimicrobial resistance genes for children.

Introduction. Respiratory tract infection, which is associated with high morbidity and mortality, occurs frequently in children. At present, the main diagnostic method is culture. However, the low pathogen detection rate of the culture approach prevents timely and accurate diagnosis. Fortunately, next-generation sequencing (NGS) can compensate for the deficiency of culture, and its application in clinical diagnostics has become increasingly available.Gap Statement. Targeted NGS (tNGS) is a platform that can select and enrich specific regions before data enter the NGS pipeline. However, the performance of tNGS in the detection of respiratory pathogens and antimicrobial resistance genes (ARGs) in infections in children is unclear.Aim and methodology. In this study, we estimated the performance of tNGS in the detection of respiratory pathogens and ARGs in 47 bronchoalveolar lavage fluid (BALF) specimens from children using conventional culture and antimicrobial susceptibility testing (AST) as the gold standard.Results. RPIP (Respiratory Pathogen ID/AMR enrichment) sequencing generated almost 500 000 reads for each specimen. In the detection of pathogens, RPIP sequencing showed targeted superiority in detecting difficult-to-culture bacteria, including Mycoplasma pneumoniae. Compared with the results of culture, the sensitivity and specificity of RPIP were 84.4 % (confidence interval 70.5-93.5 %) and 97.7 % (95.9 -98.8%), respectively. Moreover, RPIP results showed that a single infection was detected in 10 of the 47 BALF specimens, and multiple infections were detected in 34, with the largest number of bacterial/viral coinfections. Nevertheless, there were also three specimens where no pathogen was detected. Furthermore, we analysed the drug resistance genes of specimens containing Streptococcus pneumoniae, which was detected in 25 out of 47 specimens in the study. A total of 58 ARGs associated with tetracycline, macrolide-lincosamide-streptogramin, beta-lactams, sulfonamide and aminoglycosides were identified by RPIP in 19 of 25 patients. Using the results of AST as a standard, the coincidence rates of erythromycin, tetracycline, penicillin and sulfonamides were 89.5, 79.0, 36.8 and 42.1 %, respectively.Conclusion. These results demonstrated the superiority of RPIP in pathogen detection, particularly for multiple and difficult-to-culture pathogens, as well as in predicting resistance to erythromycin and tetracycline, which has significance for the accurate diagnosis of pathogenic infection and in the guidance of clinical treatment.

Beneficial effects of red and blue light on potato leaf antioxidant capacity and tuber bulking.

Artificial light application is an effective method for promoting potato production in indoor facilities. In this study, we assessed the effects of different combinations of red (R) and blue (B) light application on potato leaf and tuber growth. Potato plantlets were transplanted under W (white light, control), RB5-5 (50% R + 50% B), RB3-7 (30% R + 70% B to 70% R + 30% B) and RB1-9 (10% R + 90% B to 90% R + 10% B), and ascorbic acid (AsA) metabolism in leaves and cytokinin (CTK), auxin (indole-3-acetic acid, IAA), abscisic acid (ABA), and gibberellin (GA) levels in tubers were measured. At 50 days of treatment, potato leaves had significantly higher L-galactono-1,4-lactone dehydrogenase (GalLDH) activity and utilized AsA faster under RB1-9 treatment than under RB3-7 treatment. CTK/IAA and ABA/GA ratios in large tubers under W treatment did not differ significantly from those under RB1-9 treatment, which had higher levels than those under RB5-5 and RB3-7 treatment at 50 days. However, under RB1-9 treatment, total leaf area decreased rapidly from 60 to 75 days compared with plants under RB3-7 treatment. Tuber dry weight per plant under W and RB5-5 treatment approached a plateau at 75 days. At 80 days, RB3-7 treatment significantly improved ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase activity compared with RB1-9 treatment. RB1-9 treatment with a high ratio of blue light increased CTK/IAA and ABA/GA to improve tuber bulking at 50 days, while RB3-7 treatment with a high ratio of red light stimulated AsA metabolic pathway to delay leaf oxidation and maintain tuber biomass accumulation at 80 days. For the indoor potato cultivation, RB3-7 treatment had a higher proportion of medium-sized tubers, thus being a suitable light treatment.

New insights into the occurrence of continuous cropping obstacles in pea (Pisum sativum L.) from soil bacterial communities, root metabolism and gene transcription.

BACKGROUND: Continuous cropping is a significant obstacle to sustainable development in the pea (Pisum sativum L.) industry, but the underlying mechanisms of this remain unclear. In this study, we used 16 S rDNA sequencing, transcriptomics, and metabolomics to analyze the response mechanism of roots and soil bacteria to continuous cropping and the relationship between soil bacteria and root phenotypes of different pea genotypes (Ding wan 10 and Yun wan 8). RESULTS: Continuous cropping inhibited pea growth, with a greater effect on Ding wan 10 than Yun wan 8. Metabolomics showed that the number of differentially accumulated metabolites (DAMs) in pea roots increased with the number of continuous cropping, and more metabolic pathways were involved. Transcriptomics revealed that the number of differentially expressed genes (DEGs) increased with the number of continuous cropping. Continuous cropping altered the expression of genes involved in plant-pathogen interaction, MAPK signal transduction, and lignin synthesis pathways in pea roots, with more DEGs in Ding wan 10 than in Yun wan 8. The up-regulated expression of genes in the ethylene signal transduction pathway was evident in Ding wan 10. Soil bacterial diversity did not change, but the relative abundance of bacteria significantly responded to continuous cropping. Integrative analysis showed that the bacteria with significant relative abundance in the soil were strongly associated with the antioxidant synthesis and linoleic acid metabolism pathway of pea roots under continuous cropping once. Under continuous cropping twice, the bacteria with significant relative abundance changes were strongly associated with cysteine and methionine metabolism, fatty acid metabolism, phenylpropanoid biosynthesis, terpenoid backbone biosynthesis, linoleic acid, and amino sugar and nucleotide sugar metabolism. CONCLUSION: Ding wan 10 was more sensitive to continuous cropping than Yun wan 8. Continuous cropping times and pea genotypes determined the differences in root metabolic pathways. There were common metabolic pathways in the two pea genotypes in response to continuous cropping, and the DEGs and DAMs in these metabolic pathways were strongly associated with the bacteria with significant changes in relative abundance in the soil. This study provides new insights into obstacles to continuous cropping in peas.

Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel.

BACKGROUND: Intervertebral degenerative disc (IDD) disease is one of the most common clinical conditions causing low back pain. The main objective of this study was to investigate the repair effect of platelet-rich plasma (PRP) and ferulic acid (FA) hydrogel compound on degenerative discs in rats in combination with bioengineering technology, which may provide a strong theoretical basis for the future treatment of IDD. METHODS: Forty-five male Sprague-Dawley rats were randomly divided into groups A-F; MRI was performed in each group at 0, 4, and 8 weeks after injection; and disc tissues were obtained after executing the animals. The histomorphology, apoptosis, and protein synthesis of intervertebral discs in each group were observed by hematoxylin-eosin, Masson, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and Western blot. RESULTS: The release concentration of all groups reached the peak at 12 hours, and the highest concentration was found in the hydrogel/PRP/FA group at the same time. The MTT assay showed that hydrogel/PRP/FA is well-cytocompatible. The results of animal experiments show that hydrogel/PRP/FA has a good effect on degenerative intervertebral disc in rats. CONCLUSION: PRP/FA-rich hydrogel compound plays an active role in promoting extracellular matrix synthesis, strengthening and repairing degenerated intervertebral discs in rats.

Delayed application of N fertilizer mitigates the carbon emissions of pea/maize intercropping via altering soil microbial diversity.

Strategies to reduce carbon emissions have been a hotspot in sustainable agriculture production. The delayed N fertilizer application had the potential to reduce carbon emissions in pea (Pisum sativum L.)/maize (Zea mays L.) intercropping, but its microbial mechanism remains unclear. In this study, we investigated the effects of delayed N fertilizer application on CO2 emissions and soil microbial diversity in pea/maize intercropping. The soil respiration (Rs) rates of intercropped pea and intercropped maize were decreased by 24.7% and 25.0% with delayed application of N fertilizer, respectively. The total carbon emissions (TCE) of the pea/maize intercropping system were also decreased by 21.1% compared with that of the traditional N fertilizer. Proteobacteria, Bacteroidota, and Chloroflexi were dominant bacteria in pea and maize strips. Heatmap analysis showed that the soil catalase activity at the pea flowering stage and the soil Ν Η 4 + - Ν at the maize silking stage contributed more to the variations of bacterial relative abundances than other soil properties. Network analysis demonstrated that Rs was positively related to the relative abundance of Proteobacteria and Bacteroidota, while negatively related to the relative abundance of Chloroflexi in the pea/maize intercropping system. Overall, our results suggested that the delayed application of N fertilizer combined with the pea/maize intercropping system altered soil bacterial community diversity, thereby providing novel insights into connections between soil microorganisms and agricultural carbon emissions.

No-till and nitrogen fertilizer reduction improve nitrogen translocation and productivity of spring wheat (Triticum aestivum L.) via promotion of plant transpiration.

Excessive nitrogen (N) fertilizer has threatened the survivability and sustainability of agriculture. Improving N productivity is promising to address the above issue. Therefore, the field experiment, which investigated the effect of no-till and N fertilizer reduction on water use and N productivity of spring wheat (Triticum aestivum L.), was conducted at Wuwei experimental station in northwestern China. There were two tillage practices (conventional tillage, CT; and no-till with previous plastic film mulching, NT) and three N fertilizer rates (135 kg N ha-1, N1; 180 kg N ha-1, N2; and 225 kg N ha-1, N3). The results showed that NT lowered soil evaporation (SE) by 22.4% while increasing the ratio of transpiration to evapotranspiration (T/ET) by 13.6%, compared with CT. In addition, NT improved the total N accumulation by 11.5% and enhanced N translocation (NT) quantity, rate, and contribution by a range of 6.2-23.3%. Ultimately, NT increased grain yield (GY), N partial factor productivity, and N harvest index by 13.4, 13.1, and 26.0%, respectively. Overall, N1 increased SE (13.6%) but decreased T/ET (6.1%) compared with N3. While, N2 enhanced NT quantity, rate, and contribution by a range of 6.0-15.2%. With the integration of NT, N2 achieved the same level of GY and N harvest index as N3 and promoted N partial factor productivity by 11.7%. The significant positive correlation of NT relative to T/ET and GY indicated that improving T/ET was essential for achieving higher NT. Therefore, we concluded that no-till coupled with N fertilizer rate at 180 kg N ha-1 was a preferable management option to boost the N productivity of spring wheat in arid areas.

Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid.

BACKGROUND: The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure for the simultaneous detection of DNA and RNA pathogens. METHODS: We generated thirteen mock CSF samples with four representative pathogens of encephalitis. Each sample was subjected to ten different methods by varying sample pretreatment/nucleic acid extraction (microbial DNA, total DNA, total NA, total RNA, Whole Transcriptome Amplification (WTA)) and library generation (Illumina or NEB). Negative extraction controls (NECs) were used for each method variation. RESULTS: We found that the quality of mNGS sequencing reads was higher from the NEB kit for library generation. Microbial DNA and total RNA increased microbial deposition by depleting the host DNA. Methods total NA and total RNA can detect gram-positive, gram-negative, RNA and DNA pathogens. We applied mNGS, including total NA and NEB library generation, to CSF samples from five patients diagnosed with infectious encephalitis and correctly determined all pathogens identified in clinical etiological tests. CONCLUSIONS: Our findings suggested that total nucleic acid extraction combined with NEB library generation is the most effective mNGS procedure in CSF pathogen detection. The optimization of positive criteria and databases can improve the specificity and sensitivity of mNGS diagnosis. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR1800015425 (29/03/2018), https://www.chictr.org.cn/edit.aspx?pid=26292&htm=4 .

Energy budgeting, carbon budgeting, and carbon footprints of straw and plastic film management for environmentally clean of wheat-maize intercropping system in northwestern China.

Modern agricultural production is an energy- and carbon-intensive system. Enhancing energy and carbon efficiencies and reducing carbon footprints are important issues of sustainable development in modern agriculture. This study aimed to comprehensively assess energy and carbon budgeting and carbon footprints in wheat-maize intercropping, monoculture maize, and monoculture wheat with straw and plastic film management approaches, as based on a field experiment conducted in northwestern China. The results showed that intercropping had a greater grain yield by 12.8% and 131.0% than monoculture maize and wheat, respectively. Intercropping decreased energy and carbon inputs, increased energy and carbon outputs, thus improving energy and carbon efficiency, compared to monoculture maize. Intercropping reduced carbon footprint (CF) and yield-scale on the carbon footprint (CFy) via decreasing soil CO2 equivalent emissions over monoculture maize. For the intercropping treatments, NTSMw/NTm (no-tillage with straw mulching and residual plastic film re-mulching) and NTSSw/NTm (no-tillage with straw standing and residual plastic film re-mulching) treatments increased grain yields by 14.9% and 13.8% over CTw/CTm (conventional tillage with no straw returning and annual new plastic film mulching). The lower energy inputs and higher energy outputs were observed in NTSMw/NTm and NTSSw/NTm treatments, thus, NTSMw/NTm and NTSSw/NTm had greater energy use efficiency by 36.9% and 34.9% than CTw/CTm. NTSMw/NTm and NTSSw/NTm treatments decreased carbon inputs and increased carbon outputs, thus improving carbon efficiency by 56.6% and 53.1%, compared to CTw/CTm. NTSMw/NTm and NTSSw/NTm treatments decreased CF by 16.8% and 14.3%, and decreased CFy by 27.6% and 24.8% compared to CTw/CTm, respectively, because of the decrease in soil CO2 equivalent emissions. Our study indicated that system productivity, as well as energy and carbon efficiencies were enhanced, and carbon footprints were reduced by NTSMw/NTm and NTSSw/NTm treatments, and NTSMw/NTm had a more robust effect, indicating this treatment is the most sustainable cropping system in arid areas.

Optimized nitrogen rate, plant density, and irrigation level reduced ammonia emission and nitrate leaching on maize farmland in the oasis area of China.

Nitrogen fertilizers play a key role in crop production to meet global food demand. Inappropriate application of nitrogen fertilizer coupled with poor irrigation and other crop management practices threaten agriculture and environmental sustainability. Over application of nitrogen fertilizer increases nitrogen gas emission and nitrate leaching. A field experiment was conducted in China's oasis irrigation area in 2018 and 2019 to determine which nitrogen rate, plant density, and irrigation level in sole maize (Zea mays L.) cropping system reduce ammonia emission and nitrate leaching. Three nitrogen rates of urea (46-0-0 of N-P2O5-K2O), at (N0 = 0 kg N ha-1, N1 = 270 kg N ha-1, and N2 = 360 kg N ha-1) were combined with three plant densities (D1 = 75,000 plants/ha-1, D2 = 97,500 plants/ha-1, and D3 = 120,000 plants/ha-1) with two irrigation levels (W1 = 5,250 m3/hm2 and W2 = 4,740 m3/hm2) using a randomized complete block design. The results showed that, both the main and interaction effects of nitrogen rate, plant density, and irrigation level reduced nitrate leaching (p < 0.05). In addition, irrigation level × nitrogen rate significantly (p < 0.05) reduced ammonia emission. Nitrate leaching and ammonia emission decreased with higher irrigation level and higher plant density. However, high nitrogen rates increased both nitrate leaching and ammonia emission. The study found lowest leaching (0.35 mg kg-1) occurring at the interaction of 270 kg N ha-1 × 120,000 plants/ha-1 × 4,740 m3/hm2, and higher plant density of 120,000 plants/ha-1 combined with 0 kg N ha-1 and irrigation level of 5,250 m3/hm2 recorded the lowest ammonia emission (0.001 kg N)-1. Overall, ammonia emission increased as days after planting increased while nitrate leaching decreased in deeper soil depths. These findings show that, though the contributory roles of days after planting, soil depth, amount of nitrogen fertilizer applied and year of cultivation cannot be undermined, it is possible to reduce nitrate leaching and ammonia emission through optimized nitrogen rate, plant density and regulated irrigation for agricultural and environmental sustainability.

N-fertilizer postponing application improves dry matter translocation and increases system productivity of wheat/maize intercropping.

Intercropping increases the grain yield to feed the ever-growing population in the world by cultivating two crop species on the same area of land. It has been proven that N-fertilizer postponed topdressing can boost the productivity of cereal/legume intercropping. However, whether the application of this technology to cereal/cereal intercropping can still increase grain yield is unclear. A field experiment was conducted from 2018 to 2020 in the arid region of northwestern China to investigate the accumulation and distribution of dry matter and yield performance of wheat/maize intercropping in response to N-fertilizer postponed topdressing application. There were three N application treatments (referred as N1, N2, N3) for maize and the total amount were all 360 kg N ha-1. N fertilizer were applied at four time, i.e. prior to sowing, at jointing stage, at pre-tasseling stage, and at 15 days post-silking stage, respectively. The N3 treatment was traditionally used for maize production and allocations subjected to these four stages were 2:3:4:1. The N1 and N2 were postponed topdressing treatments which allocations were 2:1:4:3 and 2:2:4:2, respectively. The results showed that the postponed topdressing N fertilizer treatments boosted the maximum average crop growth rate (CGR) of wheat/maize intercropping. The N1 and N2 treatments increased the average maximum CGR by 32.9% and 16.4% during the co-growth period, respectively, and the second average maximum CGR was increased by 29.8% and 12.6% during the maize recovery growth stage, respectively, compared with the N3 treatment. The N1 treatment was superior to other treatments, since it increased the CGR of intercropped wheat by 44.7% during the co-growth period and accelerated the CGR of intercropped maize by 29.8% after the wheat had been harvested. This treatment also increased the biomass and grain yield of intercropping by 8.6% and 33.7%, respectively, compared with the current N management practice. This yield gain was primarily attributable to the higher total translocation of dry matter. The N1 treatment increased the transfer amount of intercropped wheat by 28.4% from leaf and by 51.6% from stem, as well as increased the intercropped maize by 49.0% of leaf, 36.6% of stem, and 103.6% of husk, compared to N3 treatment, respectively. Integrated the N fertilizer postponed topdressing to the wheat/maize intercropping system have a promotion effect on increasing the translocation of dry matter to grain in vegetative organs. Therefore, the harvest index of intercropped wheat and maize with N1 was 5.9% and 5.3% greater than that of N3, respectively. This demonstrated that optimizing the management of N fertilizer can increase the grain yield from wheat/maize intercropping via the promotion of accumulation and translocation of dry matter.

Photosynthetic Physiological Characteristics of Water and Nitrogen Coupling for Enhanced High-Density Tolerance and Increased Yield of Maize in Arid Irrigation Regions.

To some extent, the photosynthetic traits of developing leaves of maize are regulated systemically by water and nitrogen. However, it remains unclear whether photosynthesis is systematically regulated via water and nitrogen when maize crops are grown under close (high density) planting conditions. To address this, a field experiment that had a split-split plot arrangement of treatments was designed. Two irrigation levels on local traditional irrigation level (high, I2, 4,050 m3 ha-1) and reduced by 20% (low, I1, 3,240 m3 ha-1) formed the main plots; two levels of nitrogen fertilizer at a local traditional nitrogen level (high, N2, 360 kg ha-1) and reduced by 25% (low, N1, 270 kg ha-1) formed the split plots; three planting densities of low (D1, 7.5 plants m-2), medium (D2, 9.75 plants m-2), and high (D3, 12 plants m-2) formed the split-split plots. The grain yield, gas exchange, and chlorophyll a fluorescence of the closely planted maize crops were assessed. The results showed that water-nitrogen coupling regulated their net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), quantum yield of non-regulated non-photochemical energy loss [Y(NO)], actual photochemical efficiency of PSII [Y(II)], and quantum yield of regulated non-photochemical energy loss [Y(NPQ)]. When maize plants were grown at low irrigation with traditional nitrogen and at a medium density (i.e., I1N2D2), they had Pn, Gs, and Tr higher than those of grown under traditional treatment conditions (i.e., I2N2D1). Moreover, the increased photosynthesis in the leaves of maize in the I1N2D2 treatment was mainly caused by decreased Y(NO), and increased Y(II) and Y(NPQ). The coupling of 20%-reduced irrigation with the traditional nitrogen application boosted the grain yield of medium density-planted maize, whose Pn, Gs, Tr, Y(II), and Y(NPQ) were enhanced, and its Y(NO) was reduced. Redundancy analysis revealed that both Y(II) and SPAD were the most important physiological factors affecting maize yield performance, followed by Y(NPQ) and NPQ. Using the 20% reduction in irrigation and traditional nitrogen application at a medium density of planting (I1N2D2) could thus be considered as feasible management practices, which could provide technical guidance for further exploring high yields of closely planted maize plants in arid irrigation regions.

Integrated farming with intercropping increases food production while reducing environmental footprint.

Food security has been a significant issue for the livelihood of smallholder family farms in highly populated regions and countries. Industrialized farming in more developed countries has increased global food supply to meet the demand, but the excessive use of synthetic fertilizers and pesticides has negative environmental impacts. Finding sustainable ways to grow more food with a smaller environmental footprint is critical. We developed an integrated cropping system that incorporates four key components: 1) intensified cropping through relay planting or intercropping, 2) within-field strip rotation, 3) soil mulching with available means, such as crop straw, and 4) no-till or reduced tillage. Sixteen field experiments, conducted with a wide range of crop inputs over 12 consecutive years (2006 to 2017), showed that the integrated system with intercropping generates significant synergies-increasing annual crop yields by 15.6 to 49.9% and farm net returns by 39.2% and decreasing the environmental footprint by 17.3%-when compared with traditional monoculture cropping. We conclude that smallholder farmers can achieve the dual goals of growing more food and lowering the environmental footprint by adopting integrated farming systems.

Attenuating the Variability of Lipids Is Beneficial for the Hypertension Management to Reduce the Cardiovascular Morbidity and Mortality in Older Adults.

Objective: To investigate the beneficial of attenuating the variability of lipids to the hypertension management in older adults. Methods: Between April 2008 and November 2010, 1,244 hypertensive patients aged ≥60 years were recruited and randomized into placebo and rosuvastatin groups. Outcomes and inter-visit plasma lipids variability were assessed. Results: Over an average follow-up of 83.5 months, the coefficients of variation (CVs) in total cholesterol (TCHO), triglycerides, high-density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c) were significantly lower in the rosuvastatin group than the placebo group (p < 0.05). The risks of composite cardiovascular event, myocardial infarction, coronary revascularization, heart failure, total stroke, ischemic stroke, cardiovascular death, and all-cause death were significantly lower in the rosuvastatin group than the placebo group (all p < 0.05). The differences in the risks were significantly diminished after the CVs for TCHO, triglycerides, HDL-c, and LDL-c were separately included as confounders. One-SD of CVs for TCHO, triglycerides, HDL-c, and LDL-c increment were significantly associated with the risks of composite cardiovascular event, myocardial infarction, heart failure, total stroke, ischemic stroke, cardiovascular death, and all-cause death, respectively (all p < 0.05). Conclusions: Rosuvastatin significantly attenuated the intra-visit variability in lipids and decreased the risk of cardiovascular mortality and morbidity. Controlling the variability of lipids is as important as antihypertensive treatment to reduce the cardiovascular morbidity and mortality in the management of older hypertensive patients. Clinical Trial Registration: ChiCTR.org.cn, ChiCTR-IOR-17013557.

Improving wheat grain yield via promotion of water and nitrogen utilization in arid areas.

Crop yield is limited by water and nitrogen (N) availability. However, in Hexi Corridor of northwestern China, water scarcity and excessive fertilizer N in wheat (Triticum aestivum L.) production causes serious conflicts between water and N supply and crop demand. A field experiment was conducted from 2016 to 2018 to evaluate whether reducing of irrigation and fertilizer N will reduce grain yield of wheat. There were two irrigation quotas (192 and 240 mm) and three fertilizer N rates (135, 180, and 225 kg N ha-1). The results showed that reducing irrigation to 192 mm and N rate to 180 kg N ha-1 reduced water uptake, water uptake efficiency, and N uptake of spring wheat as compared to local practice (i.e., 240 mm irrigation and 225 kg N ha-1 fertilizer). Whereas, it improved water and N utilization efficiency, and water and N productivity. Consequently, the irrigation and N rate reduced treatment achieved the same quantity of grain yield as local practice. The path analysis showed that interaction effect between irrigation and N fertilization may attributable to the improvement of grain yield with lower irrigation and N rate. The enhanced water and N utilization allows us to conclude that irrigation quota at 192 mm coupled with fertilizer N rate at 180 kg N ha-1 can be used as an efficient practice for wheat production in arid irrigation areas.

Straw and residual film management enhances crop yield and weakens CO2 emissions in wheat-maize intercropping system.

Higher CO2 emissions and lower crop productivity are becoming thorny problems and restricted sustainable development of agriculture in arid inland areas. Intercropping has been shown to enhance crop productivity. However, Intercropping generally requires more input that led to an increase in CO2 emissions. It is unknown whether designing tillage and film mulching in reduction could decrease soil CO2 emissions in intercropping. Therefore, we integrated no tillage combined with residual film mulching and straw returning into wheat-maize intercropping. The maximal soil CO2 fluxes (Fs) with intercropping was decreased by 12-21% compared to sole maize. Residual film mulching combined with straw returning (NTSMI) significantly reduced average Fs during the entire period of crop growth by 14-15%, compared with the conventional tillage (CTI). Soil CO2 emissions (CE) with intercropping was 18-20% less than that with sole maize and the NTSMI reduced CE by 12-16% compared to the CTI. The NTSMI boosted total grain yields (GY) by 14-17%, compared with the CTI. Wheat-maize intercropping significantly enhanced soil CO2 emission efficiency (CEE) by 33-41% in comparison to sole maize, and CEE with NTSMI was increased by 29-40% than that of CTI. A quadratic function for aboveground biomass (BA) combined with two linear functions for soil temperature (Ts) and soil water-filled pore space (WFPS) was suitable for the monitored results. A multiple regression model composed of the above three factors can explain 73-91% of the Fs variation. Crop biomass accumulation at the time of maximal Fs was less with intercropping compared with sole maize. The structural equation indicated that the BA synergistic effect on CEE through combining negative effects on CE and positive effects on GY in intercropping. In conclusion, no tillage with straw returning and residual film mulching in wheat-maize intercropping was confirmed to be an optimum management practice to reducing soil CO2 emissions and enhancing soil CO2 emission efficiency in arid inland agroecosystem.

No Tillage With Plastic Re-mulching Maintains High Maize Productivity via Regulating Hydrothermal Effects in an Arid Region.

Plastic is a valuable mulching measure for increasing crop productivity in arid environments; however, little is known about the main mechanism by which this valuable technology actuates spatial-temporal changes in soil hydrothermal effect. So a 3-year field experiment was conducted to optimize soil hydrothermal effect of maize field with three plastic mulched management treatments: (1) no tillage with plastic re-mulching (NM), (2) reduced tillage with plastic mulching (RM), and (3) conventional tillage with annual new plastic mulching (CM). The results showed that NM treatment increased soil water content by 6.6-8.4% from maize sowing to seedling stage, than did CM, and it created a good soil moisture environment for sowing of maize. Also, NM had greater soil water content by 4.8-5.6% from maize silking to early-filling stage than had CM, and it made up for the abundant demand of soil moisture for the vigorous growth of maize filling stage. The NM treatment increased water consumption (WC) before maize big-flare stage, decreased WC from big-flare to early-filling stage, and increased WC after early-filling stage. So NM treatment effectively coordinated water demand contradiction of maize at entire growing season. NM decreased soil accumulated temperature (SAT) by 7.0-13.0% at maize sowing to early-filling stage than did CM, but NM had little influence on the SAT during filling stage. In particular, the treatment on NM had smaller absolute values of air-soil temperature differences than RM and CM treatments during maize filling stage, indicating that NM treatment maintains the relative stability of soil temperature for ensuring grain filling of maize. The NM treatment allowed the maize to grow in a suitable hydrothermal status and still maintained high yield. In addition, NM treatment obtained higher net income and rate of return by 6.4-11.0% and 44.1-54.5%, respectively, than did CM, because NM treatment mainly decreased the input costs for plastic and machine operations. Therefore, the NM treatment can be recommended as a promising technique to overcome simultaneous heat stress and water shortage in arid environments.

Strip width ratio expansion with lowered N fertilizer rate enhances N complementary use between intercropped pea and maize.

Maize (Zea mays L.)/pea (Pisum sativum L.) strip intercropping is considered a promising cropping system to boost crop productivity. The 3-year (2009-2011) field experiment was conducted at Wuwei, northwest China, with two maize to pea strip width ratios (80:80 cm and 120:80 cm), each under three N fertilizer rates (0, 90 and 135 kg N ha-1 for pea, and 0, 300, and 450 kg N ha-1 for maize). The results showed that expanding maize to pea strip width ratio from 80:80 cm to 120:80 cm coupled with a reduction of N fertilizer rate intensified N competition and improved N compensation. The apparent N recovery and N utilization efficiency of intercropped pea with strip width ratio of 120:80 cm were increased by 8.0% and 8.9% compared to strip width ratio of 80:80 cm. Compared to high N rate, the two indicators of intercropped pea with lowered N rate were increased by 10.0% and 6.0%. For intercropped maize, the two indicators were increased by 6.8% and 5.1%, with strip width ratio of 120:80 cm compared to 80:80 cm. Also, they were improved by 9.7% and 11.5%, with lowered N rate compared to high N rate. Consequently, the grain yield of pea and maize in the 120:80 cm pattern was improved by 11.9% and 7.7% compared to 80:80 cm. We concluded that expanding maize to pea strip ratio coupled with N fertilizer reduction can optimize N complementary use.

Assessing the effectiveness of statin therapy for alleviating cerebral small vessel disease progression in people ≥75 years of age.

BACKGROUND: Statins have been recommended by several guidelines as the primary prevention medication for cardiovascular diseases. However, the benefits of statin therapy for cerebral small vessel disease (CSVD), particularly in adults ≥75 years of age, have not been fully evaluated. METHODS: We analyzed the data from a prospective population-based cohort study and a randomized, double-blind, placebo-controlled clinical trial to determine whether statin therapy might aid in slowing the progression of CSVD in adults ≥75 years of age. For the cohort study, 827 participants were considered eligible and were included in the baseline analysis. Subsequently, 781 participants were included in follow-up analysis. For the clinical trial, 227 participants were considered eligible and were used in the baseline and follow-up analyses. RESULTS: The white matter hyperintensities (WMH) volume, the WMH-to-intracranial volume (ICV) ratio, the prevalence of a Fazekas scale score ≥ 2, lacunes, enlarged perivascular spaces (EPVS), and microbleeds were significantly lower in the statin group than the non-statin group at baseline in the cohort study (all P < 0.05). During the follow-up period, in both the cohort and clinical trial studies, the WMH volume and WMH-to-ICV ratio were significantly lower in the statin/rosuvastatin group than the non-statin/placebo group (all P < 0.001). Statin therapy was associated with lower risk of WMH, lacunes, and EPVS progression than the non-statin therapy group after adjustment for confounders (all P < 0.05). There was no statistically significant difference in the risk of microbleeds between the statin and non-statin therapy groups (all, P > 0.05). CONCLUSIONS: Our findings indicated that statin therapy alleviated the progression of WMH, lacunes, and EPVS without elevating the risk of microbleeds. On the basis of the observed results, we concluded that statin therapy is an efficient and safe intervention for CSVD in adults ≥75 years of age. TRIAL REGISTRATION: Chictr.org.cn: ChiCTR-IOR-17013557 , date of trial retrospective registration November 27, 2017 and ChiCTR-EOC-017013598 , date of trial retrospective registration November 29, 2017.