MiR-548c-3p inhibits the proliferation, migration and invasion of human breast cancer cell by targeting E2F3.

MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progression of breast cancer. Our results indicate that up-regulation of miR-548 significantly inhibits cell proliferation, migration andinvasion, and induces apoptosis in breast cancer cells. Further studies showed that miR-548 could specifically inhibit E2F3 expression. Moreover, rescue test showed that up-regulation of E2F2 could reverse the effect of miR-548 on proliferation, migration, invasion and apoptosis of breast cancer cells. In general, miR-548 could improve the progression of breast cancer. By targeting E2F2, which may make a potential target for the treatment of breast cancer.

[Chemical constituents from whole plants of Valeriana hardwickii].

Chemical investigation of the whole plants of Valeriana hardwickii has led to the isolation of 11 flavones and 2 monoterpe- noids by using various chromatographic techniques including column chromatography on silica gel and Sephadex LH-20, preparative TLC, and preparative HPLC. Their structures were identified by spectroscopic data analysis as syzalterin (1), 6-methylapigenin (2), 5-hydroxy-7,4'-dimethoxyflavone (3), genkwanin (4), acacetin (5), apigenin (6), quercetin (7), tricin (8), (-)-farrerol (9), sosakuranetin (10), 5,3',4'-trihydroxy-7-methoxyflavanone (11), (-)-bornyl ferulate ( 12) , and (-)-bornyl caffeate ( 13). All compounds were isolated from this plant for the first time, while compounds 1, 9-13 were obtained from this genus for the first time.

[Traditional Chinese medicine injection clinical use management model for evaluation].

OBJECTIVE: Discussion on assessment and intervention models to promote the rational use of medicines of Chinese medicine injection effect. METHOD: Using systematic prescription assessment and intervention work mode, formed expert group guide established assessment standard, developed the prescription audit specification, and extracted all Chinese medicine injection prescription of outpatient 2010 first quarter (in front of intervention) and 2011 first quarter (behind intervention), respectively for 2 543 and 3 122. RESULT: The percent of the non-indication of medication in front of intervention outpatient fell from 3.44% to behind intervention of 2.66% (P<0.05), the percent of contraindicated medication in front of intervention outpatient fell from 2. 14% to behind intervention of 1.33% (P<0.05), the incidence of adverse reactions in front of intervention outpatient fell from 1.01% to behind intervention of 0.29% (P<0.05). CONCLUSION: The modes of prescription assessment and intervention have obvious results for the promotion of rational use of traditional Chinese medicine injections.

[Effect on different adding means of calcium peroxide to control phosphorus release from sediment].

Calcium peroxide is applied to sediment remediation. Based on laboratory-simulated test, trend of soluble reactive phosphorus (SRP) concentration in overlying was measured after two means of calcium peroxide treatment, mixing and capping. Transformation among different phosphorus fractions in the sediments was studied to analyze the distinguished effect. The results show that SRP concentration in overlying water presents different trend after mixing and capping of calcium peroxide. SRP concentration of the former decreased at first and increased subsequently, while that of the latter kept decreasing and average release velocity of SRP was 6.077 mg/(m2 x d) and -0.879 mg/(m2 x d) respectively. The transformation of phosphorus fractions in the sediments shows that diverse change of iron-bound phosphate are deemed to cause the effect under different adding options of calcium peroxide.