ABSTRACT
In this study, the total length of mitochondrial genome of Duroc pig is 16,731 bp, including 34.66% A, 26.27% C, 25.74% T and 13.33% G. Mitochondrial genome contains a major non-coding control region (D-Loop region), 2 ribosomal RNA genes, 13 protein-coding genes (PCGs) and 22 transfer RNA genes. ND2 selects ATT as the initiation codon, and ATA is chose as an initiation codon in ND3 and ND5, the nonstandard start codon is GTG in ND4L and the rest protein common start codon is ATG. The mitochondrial genome of Duroc pig provides an important data in genetic mechanism, which plays an important role in the three-way crossbred pigs.
Subject(s)
Genome, Mitochondrial , Sus scrofa/genetics , Animals , Base Pairing/genetics , Base Sequence , Codon/genetics , DNA, Mitochondrial/genetics , Open Reading Frames/geneticsABSTRACT
In the present study, we sequenced the complete mitochondrial genome of Wuzhishan pig, which was 16,741 bp in size and had a nucleotide composition in A and T (60.46%). The genome consisted of a major non-coding control region (D-loop region) and 37 genes, including 2 ribosomal RNA (rRNA) genes, 13 protein-coding genes (PCGs), and 22 transfer RNA (tRNA) genes. The genes in the mitochondrial genomes of Wuzhishan pig used three kinds of initiation codons (ATA, ATG, and GTG) and four kinds of termination codons (TAA, AGA, TAG, and an incomplete termination codons T-). The complete mitochondrial genome sequence of Wuzhishan pig provides an important data set for further study on genetic mechanism.
Subject(s)
Genome, Mitochondrial/genetics , Sequence Analysis, DNA , Sus scrofa/genetics , Animals , Codon , Genes, rRNA , Molecular Sequence Annotation , Molecular Sequence Data , Open Reading Frames/genetics , RNA, Transfer/geneticsABSTRACT
In this work, we reported the complete mitochondrial genome sequence of the Ningxiang pig (Human Province), which was determined through PCR-based method. The total length of the mitogenome is 16,690 bp. The overall composition of the mitogenome was estimated to be 34.70% for A, 25.81% for T, 26.18% for C, 13.30% for G, respectively, indicating that an A + T(60.52%)-rich feature occurs in the Ningxiang pig. It contains the typical structure, including 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and 1 non-coding control region (D-loop region). The arrangement of these genes was the same as that found in the Landrace pig. All the protein initiation codons are ATG, except for ND2. ND3 and ND5 are ATA, ND4L is GTG. The complete mitochondrial genome sequence of the Ningxiang pig in Human Province provides an important data for further study about genetic mechanism.
Subject(s)
DNA, Mitochondrial/chemistry , Genome, Mitochondrial , Swine/genetics , Animals , Base Composition , Base Sequence , Genes, Mitochondrial , Genome Size , Molecular Sequence Data , Sequence Analysis, DNAABSTRACT
The complete mitochondrial genome sequence of Qianshao spotted pig was first determined in this study. The mitogenome (16,700 bp) consists of 22 tRNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 control region (D-loop region). The complete mitochondrial genome sequence of the Qianshao spotted pig enriches data resource for further study in genetic mechanism.
Subject(s)
DNA, Mitochondrial/analysis , Genome, Mitochondrial , Swine/genetics , Animals , DNA, Mitochondrial/genetics , Molecular Sequence Data , Open Reading Frames/genetics , RNA, Transfer/genetics , Sequence Analysis, DNA , Swine/classificationABSTRACT
Taoyuan Black pig is the native breed of Hunan province in China. In this study, the complete nucleotide sequence of Taoyuan Black pig mitochondrial genome was first determined. Sequence analysis showed that the genome structure was in accordance with other pig breeds. It contained 22 tRNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 control region (D-loop region). The complete mitochondrial genome sequence of the Taoyuan Black pig provides an important data set for further study on genetic mechanism.
