Salicylic acid and jasmonic acid-mediated different fate of nickel phytoremediation in two populations of Alyssum inflatum Nyár.

This study investigates Ni phytoremediation and accumulation potential in the presence of salicylic acid (SA) (0, 50 and 200 µM) and jasmonic acid (JA) (0, 5 and 10 µM) in two populations of Alyssum inflatum under various nickel (Ni) doses (0, 100 and 400 µM). By measuring Ni levels in the shoots and roots, values of bioaccumulation coefficient (BAC), biological concentration factor (BCF) and translocation factor (TF) were calculated to quantify Ni accumulation and translocation between plant organs. Additionally, the amounts of histidine (His), citric acid (CA) and malic acid (MA) were explored. The results showed that plant dry weight (DW) [in shoot (29.8%, 8.74%) and in root (21.6%, 24.4%)] and chlorophyll [a (17.1%, 32.5%), b (10.1%, 30.9%)] declined in M and NM populations respectively, when exposed to Ni (400 µM). Conversely, the levels of MA [in shoot (37.0%, 32.0%) and in root (25.5%, 21.2%)], CA [in shoot (17.0%, 10.0%) and in root (47.9%, 37.2%)] and His [in shoot (by 1.59- and 1.34-fold) and in root (by 1.24- and 1.18-fold)] increased. Also, in the presence 400 µM Ni, the highest accumulation of Ni was observed in shoots of M (1392 µg/g DW) and NM (1382 µg/g DW). However, the application of SA and JA (especially in Ni 400 µM + SA 200 µM + JA 5 and 10 µM treatments) mitigated the harmful impact of Ni on physiological parameters. Also, a decreasing trend was observed in the contents of MA, CA, and His. The reduction of these compounds as important chelators of Ni caused a decrease in root-to-shoot Ni transfer and reducing accumulation in the shoots of both populations. The values of phytoremediation indices in both populations exposed to Ni (400 µM) were above one. In presence of the SA and JA, these indices showed a decreasing trend, although the values remained above one (BAC, BCF and TF > 1). Overall, the results indicated that SA and JA can reduce phytoremediation potential of the two populations through different mechanisms.

A Potential Involvement of Metallothionein in the Zinc Tolerance of Trichoderma harzianum: Experimental Findings.

Metallothioneins are a group of cysteine-rich proteins that play an important role in the homeostasis and detoxification of heavy metals. The objective of this research was to explore the significance of metallothionein in Trichoderma harzianum tolerance to zinc. At the inhibitory concentration of 1000 ppm, the fungus adsorbed 16.7 ± 0.4 mg/g of metal. The HPLC and SDS-PAGE electrophoresis data suggested that the fungus production of metallothionein was twice as high in the presence of zinc as in the control group. The examination of the genes; metallothionein expression activator (MEA) and Cu fist revealed that the MEA, with a C2H2 zinc finger domain, increased significantly in the presence of zinc. It was observed that in T. harzianum, the enhanced expression of the metallothionein gene was managed by the metallothionein activator under zinc overload conditions. According to our knowledge, this is the first report on the role of metallothionein in the resistance of T. harzianum to zinc.

Addressing food insecurity: An exploration of wheat production expansion.

Wheat plays a crucial role in global food security, serving as a vital food crop that feeds billions of people worldwide. Currently, Russia and Ukraine are responsible for exporting approximately 25% of the world's wheat, making any issues in these regions a cause for concern regarding global wheat supply. The problems faced in these areas have led to a surge in wheat prices worldwide. Consequently, it becomes necessary to explore alternative regions that can compensate for the decline in wheat production and supply. This study focuses on wheat production and yield in major producing countries, utilizing the GYGA (Global Yield Gap Atlas) protocol for predictions. The findings reveal a global wheat production gap of 270,378,793 tons. Notably, the largest gap in irrigated wheat production exists in countries like China, India, Pakistan, Turkey, Iran, Afghanistan, Uzbekistan, Egypt, and Azerbaijan. Additionally, the rainfed wheat production gap on a global scale amounts to 545,215,692 tons, with Russia, the USA, Kazakhstan, Australia, Ukraine, China, Turkey, Canada, India, and France having the most significant production gaps. Through boundary line analysis, specific criteria were identified for suitable areas of irrigated and rainfed wheat cultivation. For irrigated conditions, the temperature range of 3000 to 7000 GDD (Growing Degree Days) and a temperature seasonality of 3 were determined as favorable. Under rainfed conditions, the suitable areas encompass a temperature range of 2000 to 4000 GDD, an aridity index exceeding 600, and a temperature seasonality of 2. Thirteen countries possess extensive agricultural land within the climatic codes favorable for irrigated wheat cultivation. Approximately 50% of the agricultural lands within these countries, corresponding to the total arable area for irrigated wheat, fall within the climatic codes 3403, 5403, 5303, 4303, 5503, 5203, 3503, 3303, and 4103. China, the United States, Ukraine, Russia, and Iran are the top five countries with favorable lands for irrigated wheat cultivation. Similarly, fourteen countries have significant agricultural lands within the favorable climatic codes for rainfed wheat cultivation. Around 52% of the agricultural lands within these countries are within the climatic codes 3702, 2702, 2802, and 4602. France, Germany, Britain, Poland, and Denmark possess the highest potential to expand rainfed wheat cultivation areas within these favorable climate codes, with respective areas of 2.7, 2.6, 1.6, and 0.9 million hectares. According to the study, the North China Plain emerges as a primary region for increasing irrigated wheat production, both in terms of cultivated area and yield potential. For rainfed conditions, the European continent stands out as a significant region to enhance wheat production.

Global insight into understanding wheat yield and production through Agro-Ecological Zoning.

Global food security requires food production to be increased in the future decades. Agro-Ecological Zones (AEZ) methodology is a successful approach used in land evaluation studies to support sustainable agricultural development. This approach can facilitate finding suitable areas for wheat production on a global scale. This study was conducted based on a climate zone map, soil data, wheat cultivation area, yield, and production under irrigated and rainfed conditions, worldwide. The results for irrigated wheat indicated that there is an area of 59.5 Mha with an average yield of 4.02 t/ha which leads to the production of about 239.6 Mt of grain yield. Furthermore, climate zones (CZs) of 8002, 5203, 5302, 5403, and 8102 have the highest wheat production with an average of 20.7, 14.2, 13.3, 11.8, 11.5 Mt, respectively. The highest amount of irrigated wheat production has been achieved in soil type code 17 (Loam LF120), which has a cultivation area of around 23.6 Mha and a production of about 106.8 Mt. Rainfed wheat production is 410 Mt, and the cultivation area is 160.2 Mha. The highest rainfed wheat production with an average of 17 Mt was related to the 3702 CZ, followed by the other CZs (3802, 1303, 1203, 3602, 4602, etc.). The soil codes 11 (Loam HF120), 10 (Loam HF180), and 14 (Loam MF120) showed the highest rainfed wheat production. The findings of this study can be useful for agricultural scientists, authorities, and decision-makers around the world to find suitable lands to expand wheat cultivation and also to find new locations for increasing global wheat production to feed the increasing population in the world.

The role of γ-aminobutyric acid and salicylic acid in heat stress tolerance under salinity conditions in Origanum vulgare L.

Origanum vulgare L., a medicinal and aromatic herb, has been used for hundreds of years. This plant contains valuable chemical compounds that can be used as medicine for treatment. On the other hand, a gradual increase in the planet's average temperature could negatively affect the growth and the composition of the O. vulgare. For this reason, in this study, the effect of two protective compounds, namely salicylic acid (SA) and gamma-aminobutyric acid (GABA), on temperature and salinity stress conditions was investigated. Oregano plants were grown at 23/12°C temperature as control and 27/16°C heat stress in the greenhouse (16/8 h photoperiod) for one months. The plants were treated with GABA and SA and subjected to salt stress for 30 days. Subsequently, the plant's physiological, biochemical, and phytochemical characteristics were examined. The results showed that all studied traits (in control and treated samples) were significantly different at 27°C, from 23°C. In addition, the highest amount of thymol and carvacrol were detected from plants grown at 27°C. In regards to salinity, stressed- plants had less damage to membrane stability and H2O2 level, when treated with GABA or SA. This study revealed that both SA and GABA compounds had an exellent protective effect on temperature and salt stress on O. vulgare. Based on enzyme-pigment evaluations and secondary metabolites, SA showed a better protective effect on temperature effects and GABA in a saline environment. In general, using these compounds can provide better conditions for the growth and preservation of O. vulgare chemical compounds. However, it certainly requires more experiments to find the signal pathways involved in these processes.

Selection of High-Yielding and Stable Genotypes of Barley for the Cold Climate in Iran.

The interaction between genotypes and environments plays an important role in selecting superior genotypes for target locations. The main objectives of the present study were to analyze the effect of the genotype-by-environment interaction (GEI) and identify superior, newly developed, and promising barley genotypes for cold regions in Iran. For these purposes, a set of genotypes obtained from breeding programs for cold climates in Iran, along with two reference genotypes, were investigated at eight research stations (Tabriz, Ardabil, Arak, Miandoab, Mashhad, Jolge Rokh, Karaj, and Hamadan) during two consecutive growing seasons (2019-2020 and 2020-2021). The results of the freezing test (LT50) showed that most of the tested genotypes had significant cold tolerance at the seedling stage. Based on the additive main effect and multiplicative interaction (AMMI) analysis, environment (E) and GEI effects explained 49.44% and 16.55% of the total variation in grain yield, respectively. Using AMMI1 and AMMI2 models, G2 and G20 were found to be superior genotypes in terms of grain yield and stability. Moreover, AMMI-based stability parameters considered the G20 genotype to be the ideal genotype. A two-plot analysis of the genotype-by-environment interaction (GGE) biplot showed that the 16 experimental environments were grouped into 2 mega-environments. Of the test environments, ARK1 and KAJ2 had the highest discriminating power and representativeness ability, and these were identified as ideal environments for testing advanced genotypes for yield and stability performance during early barley breeding practices in cold areas in Iran. In conclusion, both AMMI and GGE biplot models identified several superior genotypes, among which G20, with a high average yield relative to the overall average yield and the lowest IPC1 score, was found to have high yield stability and is recommended for inclusion in breeding programs for cold climates in Iran.

Analysis of transcriptional responses in root tissue of bread wheat landrace (Triticum aestivum L.) reveals drought avoidance mechanisms under water scarcity.

In this study, high-throughput sequencing (RNA-Seq) was utilized to evaluate differential expression of transcripts and their related genes involved in response to terminal drought in root tissues of bread wheat landrace (L-82) and drought-sensitive genotype (Marvdasht). Subsets of 460 differentially expressed genes (DEGs) in drought-tolerant genotype and 236 in drought-sensitive genotype were distinguished and functionally annotated with 105 gene ontology (GO) terms and 77 metabolic pathways. Transcriptome profiling of drought-resistant genotype "L-82" showed up-regulation of genes mostly involved in Oxidation-reduction process, secondary metabolite biosynthesis, abiotic stress response, transferase activity and heat shock proteins. On the other hand, down-regulated genes mostly involved in signaling, oxidation-reduction process, secondary metabolite biosynthesis, auxin-responsive protein and lipid metabolism. We hypothesized that the drought tolerance in "L-82" was a result of avoidance strategies. Up-regulation of genes related to the deeper root system and adequate hydraulic characteristics to allow water uptake under water scarcity confirms our hypothesis. The transcriptomic sequences generated in this study provide information about mechanisms of acclimation to drought in the selected bread wheat landrace, "L-82", and will help us to unravel the mechanisms underlying the ability of crops to reproduce and keep its productivity even under drought stress.