ABSTRACT
The long-term influence of low X-ray irradiation increases lipid peroxidation (LP) in radiosensitive (bone marrow, enterocytes of small intenstine) and in relatively radioresistant blood cells (erythrocytes). The activation of antioxidant system enzymes in observed cells does not decrease LP intensity. We concluded that additional administration of alpha-tocopherol provided the decrease of the first and end products of LP in the observed tissues mostly in the beginning of the experiment. Antioxidant effect of the preparation is more significant in cells with high proliferative activity but normal activity of enzymes was not determined.
Subject(s)
Antioxidants/therapeutic use , Enterocytes/enzymology , Erythrocytes/enzymology , Intestine, Small/enzymology , Radiation Injuries, Experimental/prevention & control , Vitamin E/therapeutic use , Animals , Bone Marrow Cells/drug effects , Bone Marrow Cells/enzymology , Bone Marrow Cells/radiation effects , Catalase/metabolism , Dose-Response Relationship, Radiation , Enterocytes/drug effects , Enterocytes/radiation effects , Erythrocytes/drug effects , Erythrocytes/radiation effects , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Intestine, Small/drug effects , Intestine, Small/radiation effects , Lipid Peroxides/blood , Radiation Dosage , Radiation Injuries, Experimental/blood , Radiation Injuries, Experimental/enzymology , Rats , Superoxide Dismutase/metabolism , X-RaysABSTRACT
Activity and isoenzyme composition of certain enzymes of glycolysis of rat small intestine enterocytes was studied in the course of exposure to X radiation. Hexokinase activity was shown to increase significantly throughout the entire period of observation. Activity of pyruvate kinase and lactate dehydrogenase was inhibited at early (days 1-3) and increased at later (days 5-10) times of observation.
Subject(s)
Glycolysis/radiation effects , Intestine, Small/enzymology , Intestine, Small/radiation effects , Animals , Hexokinase/radiation effects , Intestinal Mucosa/enzymology , Intestinal Mucosa/radiation effects , Isoenzymes/radiation effects , L-Lactate Dehydrogenase/radiation effects , Male , Pyruvate Kinase/radiation effects , Radiation Tolerance , Rats , Time FactorsABSTRACT
A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.03%; the incubation time--15 min; 200-250 mg of mucosa produces 3,7 X 10(6)--4,7 X 10(6) cells.
