Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
J Basic Microbiol ; 47(6): 468-73, 2007 Dec.
Article in English | MEDLINE | ID: mdl-18072247

ABSTRACT

This work was conducted to study the removal of gamma-hexachlorocyclohexane (lindane) in a soil extract liquid medium (SE) by Streptomyces sp. M7 and to determine the influence of pH and temperature on bacterial growth and pesticide removal in this medium. When Streptomyces sp. M7 was cultured in SE supplemented with lindane 100 microg l(-1 )at different initial pH, the maximum growth was observed at pH 7 and the microorganism was not able to grow at pH 5 and 9; the highest pesticide removal (70.4%) by Streptomyces sp. M7 was noted at an initial pH of 7 at 4 weeks of incubation. The maximum removal (70% approximately) was observed when the microorganism was incubated in SE at 30 degrees C; although the optimal temperature for Streptomyces sp. M7 growth, with and without lindane, was 25 degrees C, and for the pesticide removal was 30 degrees C. The results of this study suggest that this actinomycete strain appears as an effective alternative in the remediation of lindane polluted sites.


Subject(s)
Hexachlorocyclohexane/metabolism , Streptomyces/metabolism , Biodegradation, Environmental , Complex Mixtures , Hydrogen-Ion Concentration , Soil , Soil Microbiology , Streptomyces/growth & development , Temperature
2.
J Basic Microbiol ; 46(5): 348-57, 2006.
Article in English | MEDLINE | ID: mdl-17009290

ABSTRACT

Gamma-hexachlorocyclohexane (gamma-HCH or lindane), one of the most commonly used pesticides, has been mainly used in agriculture; this pesticide is known to be highly toxic and persistent, causing serious water and soil contamination. The objective of the present work is to study the effect of low glucose concentration and the addition of lindane at different growing time on the pesticide detoxification ability of Streptomyces M7. After 96 h of incubation in synthetic medium containing glucose 0.6 g l(-1) with the addition of lindane 100 microg l(-1) at 20 h of incubation, a typical diauxic curve was obtained: glucose was the preferred substrate until 24 h, at 48 h, when the carbohydrate was depleted, the microorganism consumed the pesticide like carbon source. On the other hand, lindane removal induction was observed, which was greater when the pesticide was added to the medium at 20 h than 6 h of incubation. Between 72 and 96 h, a maximum of approximately 86% of the Cl(-) was released when lindane was added to the medium at 20 h, whereas approximately 70% and 67% Cl(-) was released in the medium when the pesticide was added at 0 and 6 h of incubation respectively. This is the first report of chloride release from inoculated medium supplemented with lindane, suggesting that the pesticide was degraded by Streptomyces sp. under aerobic conditions.


Subject(s)
Hexachlorocyclohexane/metabolism , Insecticides/metabolism , Streptomyces/metabolism , Biodegradation, Environmental , Chlorides/metabolism , Culture Media , Ecosystem , Geologic Sediments/microbiology , Glucose/metabolism , Streptomyces/growth & development , Streptomyces/isolation & purification
3.
Bioresour Technol ; 89(2): 133-8, 2003 Sep.
Article in English | MEDLINE | ID: mdl-12699931

ABSTRACT

Ninety-three wild-type isolates identified as actinomycetes were tested against 11 organochlorine pesticides (OPs): aldrin, chlordane, DDD, DDE, DDT, dieldrin, heptachlor, and heptachlor epoxides, lindane, and methoxychlor. Qualitative screening agar assays displayed 62-78% tolerance of strains to OPs. Four strains designed M4, M7, M9 and M15 were selected based on multi-OP-tolerance, and identified as members of the streptomycetes group. Different growth profiles were observed in cultures of the four selected streptomycetes cultured in synthetic medium containing 5-50 microg x l(-1) aldrin or chlordane or lindane. Increase of aldrin removal by the selected microorganisms was concomitant with the 4.8-36.0 microg x l(-1) pesticide concentration range. After 72 h of streptomycete M7 growth in synthetic medium containing 48.0 microg x l(-1) aldrin, the remaining OP concentration in the supernatant was approximately 10% of the initial concentration. Also, in stationary growth phase less than 2.5 microg x l(-1) aldrin residual concentration was detected in the medium.


Subject(s)
Hydrocarbons, Chlorinated , Insecticides/metabolism , Streptomycetaceae/physiology , Water Purification/methods , Biodegradation, Environmental , Streptomycetaceae/isolation & purification
SELECTION OF CITATIONS
SEARCH DETAIL
...